“…Biological monitoring indicates the cumulative effects of all environmental parameters on the ecosystems (Lobo et al, 2004(Lobo et al, , 2016(Lobo et al, , 2019Çelekli and Kapı, 2019;Ballesteros et al, 2020;Mbao et al, 2020;Pajunen et al, 2020). The water quality and long-term scale changes observed by using the biological assessment should be supported by physical and chemical approaches (Çelekli and Kapı, 2019;Lobo et al, 2019;Mbao et al, 2020;Pajunen et al, 2020). On the other hand, there are some challenges in biological monitoring, and they should not be ignored.…”