LHX1 as a potential biomarker regulates EMT induction and cellular behaviors in uterine corpus endometrial carcinoma

Tian, Ye; Fang, Wen; Wang, Shuo; Lv, Na

doi:10.1016/j.clinsp.2022.100103

Cited by 5 publications

(4 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…PCAT6 enhances proliferation and EMT in gastric cancer (GC) 29 , and KDM5B enhances EMT in lung cancer cell line, A549 30 , and in hepatocellular carcinoma 31 . LHX1 is involved in modulating EMT induction in uterine corpus endometrial carcinoma 32 , and LHX1-DT enhances cell proliferation in breast cancer 33 . These coordinated gene expression changes that were up-regulated by TGFβ stimulation were also confirmed by qRT-PCR (Fig.…”

Section: Resultsmentioning

confidence: 99%

CCIVR2 facilitates comprehensive identification of both overlapping and non-overlapping antisense transcripts within specified regions

Suzuki,

Sakai,

Ota

et al. 2023

Sci Rep

View full text Add to dashboard Cite

Pairs of sense and antisense transcriptions that are adjacent at their 5′ and 3′ regions are called divergent and convergent transcription, respectively. However, the structural properties of divergent/convergent transcription in different species or RNA biotypes are poorly characterized. Here, we developed CCIVR2, a program that facilitates identification of both overlapping and non-overlapping antisense transcripts produced from divergent/convergent transcription whose transcription start sites (TSS) or transcript end sites (TES) are located within a specified region. We used CCIVR2 to analyze antisense transcripts starting around the sense TSS (from divergent transcription) or ending around the sense TES (from convergent transcription) in 11 different species and found species- and RNA biotype-specific features of divergent/convergent transcription. Furthermore, we confirmed that CCIVR2 enables the identification of multiple sense/antisense transcript pairs from divergent transcription, including those with known functions in processes such as embryonic stem cell differentiation and TGFβ stimulation. CCIVR2 is therefore a valuable bioinformatics tool that facilitates the characterization of divergent/convergent transcription in different species and aids the identification of functional sense/antisense transcript pairs from divergent transcription in specified biological processes.

show abstract

Section: Resultsmentioning

confidence: 99%

CCIVR2 facilitates comprehensive identification of both overlapping and non-overlapping antisense transcripts within specified regions

Suzuki,

Sakai,

Ota

et al. 2023

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In the last year, an estimated 66,570 new cases of endometrial cancer and 12,940 deaths related to endometrial cancer occurred worldwide [29]. Unfortunately, its incidence and mortality rates will keep rising due to lifestyle changes [30]. Previous studies have shown that abnormal expression of p53 and p16 can be used to identify highrisk endometrial cancer patients with poor prognosis, but this is still poorly used in clinical practice [31]; lower expression of ER and PR is associated with increased tumour-related mortality, but does not improve the prediction of individual mortality levels [32].…”

Section: Discussionmentioning

confidence: 99%

LAMP3 is a potent uterine corpus endometrial carcinoma prognostic biomarker associated with immune behavior

Fu,

Zhou,

Geng

et al. 2024

Aging

View full text Add to dashboard Cite

Background: Uterine corpus endometrial carcinoma (UCEC) is one of the most common gynecological malignancies and its incidence and mortality continue apace. Lysosome-associated membrane protein 3 (LAMP3) is the third member of the LAMP family and its overexpression has been described to be involved in the progression of breast, ovarian and cervical cancers, but there has been an absence of research focusing on its role in UCEC. Methods: WGCNA, TIMER, LinkedOmics, GSEA, Cytoscape, Kaplan-Meier plotter, GDC, GeneMANIA, cBioPortal, PDB, RNAinter, miRNet were applied in this research. Results: Our study uncovers that LAMP3 possesses higher expression levels in UCEC compared to normal tissues, and this differential expression profile is tightly aligned with clinical and pathological features, and patients demonstrating high LAMP3 expression tend to have a shorter survival expectancy. The high expression of LAMP3 is modulated by the designated ceRNA network. LAMP3 is engaged in UCEC progression by functioning in a variety of biological roles of relevance to immunity. Furthermore, we predicted several prospering drugs based on drug sensitivity. Finally, we also constructed possible docking patterns of LAMP3 with ABCA3, RAB9A, and SGTB. Conclusions: LAMP3 is a formidable biomarker for UCEC and could be a prospective candidate for the diagnosis, treatment and prognostic assessment of UCEC.

show abstract

“…Further detailed verifications are needed in the future. Recently, an in vitro study reported that LIM1 may be a prognostic marker for EC, suggesting that LIM1 contributes to tumor grade (16). In 2013, TCGA proposed a new prognostic classification of EC by comprehensive genomic analysis.…”

Section: Discussionmentioning

confidence: 99%

LIM1 contributes to the malignant potential of endometrial cancer

et al. 2023

View full text Add to dashboard Cite

IntroductionThe incidence of endometrial cancer (EC) has been increasing worldwide. However, because there are limited chemotherapeutic options for the treatment of EC, the prognosis of advanced-stage EC is poor.MethodsGene expression profile datasets for EC cases registered in The Cancer Genome Atlas (TCGA) was reanalyzed. Highly expressed genes in advanced-stage EC (110 cases) compared with early-stage EC (255 cases) were extracted and Gene Ontology (GO) enrichment analysis was performed. Among the enriched genes, Kaplan-Meier (KM) plotter analysis was performed. Candidate genes expression was analyzed in HEC50B cells and Ishikawa cells by RT-qPCR. In HEC50B cells, LIM homeobox1 (LIM1) was knocked down (KD) and cell proliferation, migration, and invasion ability of the cells were evaluated. Xenografts were generated using LIM1-KD cells and tumor growth was evaluated. Ingenuity Pathway Analysis (IPA) of RNA-seq data using LIM-KD cells was performed. Expression of phospho-CREB and CREB-related proteins were evaluated in LIM1-KD cells by western blotting and in xenograft tissue by immunofluorescent staining. Two different CREB inhibitors were treated in HEC50B and cell proliferation was evaluated by MTT assay.ResultsReanalysis of TCGA followed by GO enrichment analysis revealed that homeobox genes were highly expressed in advanced-stage EC. Among the identified genes, KM plotter analysis showed that high LIM1 expression was associated with a significantly poorer prognosis in EC. Additionally, LIM1 expression was significantly higher in high-grade EC cell lines, HEC50B cells than Ishikawa cells. Knockdown of LIM1 showed reduced cell proliferation, migration and invasion in HEC50B cells. Xenograft experiments revealed that tumor growth was significantly suppressed in LIM1-KD cells. IPA of RNA-seq data using LIM-KD cells predicted that the mRNA expression of CREB signaling-related genes was suppressed. Indeed, phosphorylation of CREB was decreased in LIM1-KD cells and LIM1-KD cells derived tumors. HEC50B cells treated by CREB inhibitors showed suppression of cell proliferation.Conclusion and discussionCollectively, these results suggested that high LIM1 expression contributed to tumor growth via CREB signaling in EC. Inhibition of LIM1 or its downstream molecules would be new therapeutic strategies for EC.

show abstract

LHX1 as a potential biomarker regulates EMT induction and cellular behaviors in uterine corpus endometrial carcinoma

Cited by 5 publications

References 39 publications

CCIVR2 facilitates comprehensive identification of both overlapping and non-overlapping antisense transcripts within specified regions

CCIVR2 facilitates comprehensive identification of both overlapping and non-overlapping antisense transcripts within specified regions

LAMP3 is a potent uterine corpus endometrial carcinoma prognostic biomarker associated with immune behavior

LIM1 contributes to the malignant potential of endometrial cancer

Contact Info

Product

Resources

About