Exosomal miR-152-5p and miR-3681-5p function as potential biomarkers for ST-segment elevation myocardial infarction

Chen, Xiaozhu; Huang, Fang; Liu, Yunhong; Liu, Shujun; Tan, Gangwen

doi:10.1016/j.clinsp.2022.100038

Cited by 13 publications

(2 citation statements)

References 33 publications

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“…Wang et al showed that circ-SIRT1 can upregulate its host gene SIRT1 through the post-transcriptional levels of miR-3681-3p/miR-5195-3p, and this SIRT1 activation can inhibit cardiac hypertrophy and promote autophagy [38]. Exosomal miR-3681-5p may be used as a biomarker in ST-segment elevation myocardial infarction [39]. However, for miR-3177-5p and other microRNAs, there are few regulatory channels related to cardiovascular diseases, which still need further investigation.…”

Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of immune-associated, chromatin-regulating genes as biomarkers of persistent atrial fibrillation

Zhong

2023

Preprint

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Aims: Atrial fibrillation (AF) is the most common arrhythmia associated with high morbidity and mortality.Chromatin regulators an analysis of the expression and immunological correlation of CRs in pAF and normal tissues was conducted to assess their potential as diagnostic biomarkers. Methods: GSE31821, GSE411777, and GSE79768 datasets from the gene expression database, Gene Expression Omnibus, were combined into an integrated dataset for use as a training set. GSE2240 was used as a validation dataset. The merge function in R language was used to obtain the intersection of CRs and the included study data. The “Limma” software package was used to identify CR-related, differentially expressed genes (CR DEGs) in normal and pAF tissues, and the protein-protein interaction (PPI) network was used to search for hub genes. A logistic regression model was constructed based on these immHub genes to predict the occurrence of pAF. Results: We observed increased expression of 48 genes, including 29 immune-related genes. Correlation of CR DEGS and the hub genes yielded six immHub genes (RBBP4, KAT7, KANSL2, ACTB, TRRAP, and KAT2B). The AUC values in the ROC analysis were 0.861 in the training dataset and 0.83 in the validation dataset. Conclusions: Biomarkers such as RBBP4, KAT7, KANSL2, ACTB, TRRAP, and KAT2B may be associated with pAF, and relevant regulated microRNAs may be used as biomarkers or targets for the treatment of pAF. These findings could provide insights into the diagnosis, treatment, and prognosis evaluation of patients with pAF.

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Section: Discussionmentioning

confidence: 99%

Bioinformatics analysis of immune-associated, chromatin-regulating genes as biomarkers of persistent atrial fibrillation

Zhong

2023

Preprint

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“…A previous study showed that miR-152-5p is a potential biomarker for myocardial infarction (20). The Ras homolog family member A (RhoA)/Rho-associated protein kinase (ROCK) pathway interacts with numerous other signaling pathways, including the MAPK, hypoxia-inducible factor-1 and NF-κB pathways (21)(22)(23).…”

Section: Introductionmentioning

confidence: 99%

Exosomal miR‑152‑5p/ARHGAP6/ROCK axis regulates apoptosis and fibrosis in cardiomyocytes

et al. 2023

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Acute myocardial infarction (AMI) is a fatal cardiovascular disease with a high mortality rate. The discovery of effective biomarkers is crucial for the diagnosis and treatment of AMI. In the present study, miRNA sequencing and reverse transcription-quantitative polymerase chain reaction techniques revealed that the expression of exosome derived miR-152-5p was significantly downregulated in patients with AMI compared with healthy controls. A series of functional validation experiments were then performed using H9c2 cardiomyocytes. Following transfection of the cardiomyocytes using an miR-152-5p inhibitor, immunofluorescence staining of a-smooth muscle actin revealed a marked increase in fibrosis. Western blotting revealed that the expression levels of the apoptotic protein Bax, TNF-α and collagen-associated proteins were significantly increased, whereas those of the apoptosis-inhibiting factor Bcl-2 and vascular endothelial growth factor A were significantly decreased. Furthermore, the binding of Rho GTPase-activating protein 6 (ARHGAP6) to miR-152-5p was predicted using an online database and verified using a dual-luciferase reporter gene assay. The transfection of cardiomyocytes with miR-152-5p mimics was found to inhibit the activation of ARHGAP6 and Rho-associated coiled-coil containing kinase 2 (ROCK2). These results suggest that miR-152-5p targets ARHGAP6 through the ROCK signaling pathway to inhibit AMI, which implies that miR-152-5p may be a diagnostic indicator and potential target for treatment of myocardial infarction.

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Exploring miRNA function in maintaining redox mechanism of high altitude hypoxia associated maladies: An evidence based study

Rathor,

Suryakumar

2024

Advances in Redox Research

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Exosomal miR-152-5p and miR-3681-5p function as potential biomarkers for ST-segment elevation myocardial infarction

Cited by 13 publications

References 33 publications

Bioinformatics analysis of immune-associated, chromatin-regulating genes as biomarkers of persistent atrial fibrillation

Bioinformatics analysis of immune-associated, chromatin-regulating genes as biomarkers of persistent atrial fibrillation

Exosomal miR‑152‑5p/ARHGAP6/ROCK axis regulates apoptosis and fibrosis in cardiomyocytes

Exploring miRNA function in maintaining redox mechanism of high altitude hypoxia associated maladies: An evidence based study

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