Evaluation of Argentinean medicinal plants and isolation of their bioactive compounds as an alternative for the control of postharvest fruits phytopathogenic fungi

Liberto, Melina Gabriela Di; Stegmayer, María; Svetaz, Laura; Derita, Marcos Gabriel

doi:10.1016/j.bjp.2019.05.007

Cited by 14 publications

(8 citation statements)

References 11 publications

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“…Many sesquiterpenes obtained from another plant source (Drimys winteri Forst) have been reported against phytopathogens in vitro [6,25]. Particularly, Montenegro et al (2018) reported high MICs values (from 16 to 32 µg/mL) for the sesquiterpenes polygodial, drimenol, isonordrimenone, and nordrimenone against the causative agents of bacterial canker (Clavibacter michiganensis) and bacterial speck (Pseudomonas syringae pv.…”

Section: Discussionmentioning

confidence: 99%

“…Since regulations on the use of new and existing fungicides are getting stricter, there is an urgent need to find and develop new chemical entities with antifungal activities [2]. Different naturally occurring compounds [3], semisynthetic derivatives [4], chitosan-based formulations [5], and plant products, including extracts [6] or essential oils [7], have been reported as part of this strategy. Moreover, some natural products and derivatives have been synthesized with the purpose of enlarging the offer of fungicides to be used on fruits [8].…”

Section: Introductionmentioning

confidence: 99%

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Botanical Control of Citrus Green Mold and Peach Brown Rot on Fruits Assays Using a Persicaria acuminata Phytochemically Characterized Extract

Liberto

Seimandi

Fernández

et al. 2021

Plants

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Persicaria acuminata (Polygonaceae) is a perennial herb that grows in the central area of Argentina and it is commonly used by native populations to heal infected wounds and other conditions related to fungal infections. In this article, we explored the in vitro antifungal activity of its ethyl acetate extract against a panel of three fruit phytopathogenic fungi including: Penicillium digitatum, P. italicum, and Monilinia fructicola. The sesquiterpenes isolated from the extract were also evaluated against these strains, demonstrating that the dialdehyde polygodial was the responsible for this activity. In order to encourage the use of the extract rather than the pure compound, we displayed ex vivo assays using fresh oranges and peaches inoculated with P. digitatum and M. fructicola, respectively, and subsequently treated by immersion with an extract solution of 250 and 62.5 µg/mL, respectively. There were no statistically significant differences between the treatments with commercial fungicides and the extract over the control of both fruit rots. The concentration of the active compound present in the extract used on fruit experiments was determined by Gas Chromatography-Mass Spectroscopy. Finally, cytotoxicity evaluation against Huh7 cells showed that P. acuminata extract was less cytotoxic than the commercial fungicides at the assayed concentrations. After these findings we could conclude that a chemically characterized extract of P. acuminata should be further developed to treat fungal diseases in fruits from an agro-ecological model.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Botanical Control of Citrus Green Mold and Peach Brown Rot on Fruits Assays Using a Persicaria acuminata Phytochemically Characterized Extract

Liberto

Seimandi

Fernández

et al. 2021

Plants

Self Cite

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“…Plant diseases caused by phytopathogenic fungi reduce crop yields and dampen the quality and safety of agricultural products, causing severe production losses of agricultural and horticultural crops worldwide and threatening global food security and public health. , For instance, Sclerotinia sclerotiorum, a phytopathogenic fungus distributed globally, invades hundreds of crop species and is responsible for Sclerotinia stem rot of oilseed rape (Brassica napus L.), which is most devastating for its production. − The immense loss caused by phytopathogenic fungi led to the wide application of various antifungal agents. However, the misuse and overuse of many traditional fungicides for a long time have caused toxicity, altered pharmacokinetics, and ever-rising resistance .…”

Section: Introductionmentioning

confidence: 99%

Design, Synthesis, and Structure–Activity Relationship of Quinazolinone Derivatives as Potential Fungicides

Peng

Yin

et al. 2021

J. Agric. Food Chem.

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Plant diseases caused by phytopathogenic fungi reduce the yield and quality of crops. To develop novel antifungal agents, we designed and synthesized eight series of quinazolinone derivatives and evaluated their anti-phytopathogenic fungal activity. The bioassay results revealed that compounds KZL-15, KZL-22, 5b, 6b, 6c, 8e, and 8f exhibited remarkable antifungal activity in vitro. Especially, compound 6c displayed the highest bioactivity against Sclerotinia sclerotiorum, Pellicularia sasakii, Fusarium graminearum, and Fusarium oxysporum, displaying appreciable IC50 values (50% inhibitory concentration) of 2.46, 2.94, 6.03, and 11.9 μg/mL, respectively. A further mechanism interrogation revealed abnormal mycelia, damaged organelles, and changed permeability of cell membranes in S. sclerotiorum treated with compound 6c. In addition, the in vivo bioassay indicated that compound 6c possessed comparable curative and protective effects (87.3 and 90.7%, respectively) to the positive control azoxystrobin (89.5 and 91.2%, respectively) at 100 μg/mL concentration against S. sclerotiorum. This work validated the potential of compound 6c as a new and promising fungicide candidate, contributing to the exploration of potent antifungal agents.

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“…For example, tephroapollin-F, a prenylated flavonoid isolated from Tephrosia apollinea L., effectively inhibited the growth of Colletotrichum acutatum [ 8 ]. Pinostrobin and flavokawin B are bioactive compounds from Polygonum stelligerum that have been demonstrated to effectively suppress the growth of Monilinia fructicola and Rhizopus stolonifer [ 9 ]. Furthermore, antidesmone, isolated from Waltheria indica , displayed broad-spectrum antifungal activities against all tested phytopathogenic fungi with EC 50 values ranging from 0.60 to 20.15 μg/mL [ 1 ].…”

Section: Introductionmentioning

confidence: 99%

The Antifungal Mechanism of Isoxanthohumol from Humulus lupulus Linn.

Yan

et al. 2021

IJMS

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Humulus lupulus Linn. is a traditional medicinal and edible plant with several biological properties. The aims of this work were: (1) to evaluate the in vitro antifungal activity of H. lupulus ethanolic extract; (2) to study the in vitro and in vivo antifungal activity of isoxanthohumol, an isoprene flavonoid from H. lupulus, against Botrytis cinerea; and (3) to explore the antifungal mechanism of isoxanthohumol on B. cinerea. The present data revealed that the ethanolic extract of H. lupulus exhibited moderate antifungal activity against the five tested phytopathogenic fungi in vitro, and isoxanthohumol showed highly significant antifungal activity against B. cinerea, with an EC50 value of 4.32 µg/mL. Meanwhile, it exhibited moderate to excellent protective and curative efficacies in vivo. The results of morphologic observation, RNA-seq, and physiological indicators revealed that the antifungal mechanism of isoxanthohumol is mainly related to metabolism; it affected the carbohydrate metabolic process, destroyed the tricarboxylic acid (TCA) cycle, and hindered the generation of ATP by inhibiting respiration. Further studies indicated that isoxanthohumol caused membrane lipid peroxidation, thus accelerating the death of B. cinerea. This study demonstrates that isoxanthohumol can be used as a potential botanical fungicide for the management of phytopathogenic fungi.

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Evaluation of Argentinean medicinal plants and isolation of their bioactive compounds as an alternative for the control of postharvest fruits phytopathogenic fungi

Cited by 14 publications

References 11 publications

Botanical Control of Citrus Green Mold and Peach Brown Rot on Fruits Assays Using a Persicaria acuminata Phytochemically Characterized Extract

Botanical Control of Citrus Green Mold and Peach Brown Rot on Fruits Assays Using a Persicaria acuminata Phytochemically Characterized Extract

Design, Synthesis, and Structure–Activity Relationship of Quinazolinone Derivatives as Potential Fungicides

The Antifungal Mechanism of Isoxanthohumol from Humulus lupulus Linn.

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