Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro

Romero, M.E.; Freire, J. R. Murillo; Pastene, Edgar; García-Cancino, Apolinaria; Aranda, Mario; González, Carlos

doi:10.1016/j.bjp.2019.03.002

Cited by 27 publications

(24 citation statements)

References 38 publications

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“…In a set of eleven studies [45][46][47][48][49][50][51][52][53][54][55] determining an activity and morphological effect of plant extracts and plant-derived compounds on H. pylori, four of them reported a bacteriostatic activity [45][46][47][48] and six indicated a bactericidal effect [49][50][51][52][53][54] of the tested compounds. In one case, the use of this classification was avoided [55].…”

Section: Plant Extracts and Plant-derived Compoundsmentioning

confidence: 99%

“…The six studies indicated the bactericidal effect of the tested compounds on H. pylori [49][50][51][52][53][54]. A capacity to completely inhibit growth was only proven in half of them [49,51,52], which was achieved in a range of 18 h [49] to 72 h [51] with MICs.…”

Section: Plant Extracts and Plant-derived Compoundsmentioning

confidence: 99%

“…Here, a 48-h incubation with MIC (a 2 log viability decrease) and 2× MIC (a total viability reduction) of dehydrocostus lactone from Magnolia sieboldii leaves induced the formation of 49% and 94% of these morphological forms, respectively. In the remaining three articles [50,53,54], the decrease in viability of treated cells was different and ranged from 1 log after 6-h exposure to 2× MIC [53] to 3-4 logs after 12 h with a fractional inhibitory concentration (FIC) of the tested compounds [54]. In the case of two articles [53,54], an ability of the tested substances to induce cell destruction and create forms with degradative properties was indicated.…”

Section: Plant Extracts and Plant-derived Compoundsmentioning

confidence: 99%

“…The heterogeneity of the results of the research presented in this review makes it difficult to clearly judge this issue. Based on a comparison of the level of bactericidal activity, speed of action, and an ability to act on both morphological forms of H. pylori (spiral and coccoid), one can be tempted to select representatives from each group of compounds: essential oils [46] and propolis components [54] (plant compounds); SQ109 [62], 3-bromopyruvate [40] and sertraline [41] (synthetic compounds); linolenic acid and its derivatives [43,65] (fatty acids), and (PGA) m -r-(PHLG-MHH) n [68] (peptides). It is suggested that promising results obtained in vitro for planktonic H. pylori forms for these substances should be verified in vivo and for biofilm forms of these bacteria.…”

Section: Implications For Scientists and Cliniciansmentioning

confidence: 99%

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Transformation of Helicobacter pylori into Coccoid Forms as a Challenge for Research Determining Activity of Antimicrobial Substances

Krzyżek

Grande

2020

Pathogens

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Morphological variability is one of the phenotypic features related to adaptation of microorganisms to stressful environmental conditions and increased tolerance to antimicrobial substances. Helicobacter pylori, a gastric mucosal pathogen, is characterized by a high heterogeneity and an ability to transform from a spiral to a coccoid form. The presence of the coccoid form is associated with the capacity to avoid immune system detection and to promote therapeutic failures. For this reason, it seems that the investigation for new, alternative methods combating H. pylori should include research of coccoid forms of this pathogen. The current review aimed at collecting information about the activity of antibacterial substances against H. pylori in the context of the morphological variability of this bacterium. The collected data was discussed in terms of the type of substances used, applied research techniques, and interpretation of results. The review was extended by a polemic on the limitations in determining the viability of coccoid H. pylori forms. Finally, recommendations which can help in future research aiming to find new compounds with a potential to eradicate H. pylori have been formulated.

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Section: Plant Extracts and Plant-derived Compoundsmentioning

confidence: 99%

Section: Plant Extracts and Plant-derived Compoundsmentioning

confidence: 99%

Section: Plant Extracts and Plant-derived Compoundsmentioning

confidence: 99%

Section: Implications For Scientists and Cliniciansmentioning

confidence: 99%

See 2 more Smart Citations

Transformation of Helicobacter pylori into Coccoid Forms as a Challenge for Research Determining Activity of Antimicrobial Substances

Krzyżek

Grande

2020

Pathogens

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“…As mentioned above, the aliquots series obtained from the time-kill curve with the H. pylori 43504 strain were used for TEM ( Romero et al, 2019 ). Bacteria were collected by centrifugation, fixed in 2.5% glutaraldehyde, and kept at 4°C for one week until the moment they were processed.…”

Section: Methodsmentioning

confidence: 99%

Chilean Rhubarb, Gunnera tinctoria (Molina) Mirb. (Gunneraceae): UHPLC-ESI-Orbitrap-MS Profiling of Aqueous Extract and its Anti-Helicobacter pylori Activity

et al. 2021

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The full UHPLC-MS metabolome fingerprinting and anti-Helicobacter pylori effect of Gunnera tinctoria (Molina) Mirb. (Nalca) total extract (GTE) and fractions prepared from its edible fresh petioles were evaluated. The activity of G. tinctoria against H. pylori strains ATCC 45504 and J99 was assessed in vitro by means of agar diffusion assay, Minimum Inhibition Concentration (MIC), and Minimum Bactericidal Concentration (MBC), while killing curve and transmission electronic microscopy (TEM) were conducted in order to determine the effect of the plant extract on bacterial growth and ultrastructure. Additionally, the inhibitory effect upon urease was evaluated using both the Jack Bean and H. pylori enzymes. To determine which molecules could be responsible for the antibacterial effects, tentative identification was done by ultra-high performance liquid chromatography coupled with high-resolution mass spectrometry (UHPLC-Q-Orbitrap®-HR-MS). Furthermore, the total G. tinctoria extract was fractionated using centrifugal partition chromatography (CPC), giving four active fractions (1–4). It was determined that the crude extract and centrifugal partition chromatography fractions of G. tinctoria have a bactericidal effect being the lowest MIC and MBC = 32 μg/ml. In the killing curves, fraction one acts faster than control amoxicillin. In the urease assay, F3 exhibited the lowest IC50 value of 13.5 μg/ml. Transmission electronic microscopy showed that crude G. tinctoria extract promotes disruption and separation of the cellular wall and outer membrane detachment on H. pylori causing bacterial cell death.

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Pinocembrin: Insights into the Resources, Biosynthesis, Bioavailability, Safety, Biological Activities and Pharmacology

Wong,

Ahmat,

Abas

2023

Handbook of Dietary Flavonoids

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Propolis polyphenolic compounds affect the viability and structure of Helicobacter pylori in vitro

Cited by 27 publications

References 38 publications

Transformation of Helicobacter pylori into Coccoid Forms as a Challenge for Research Determining Activity of Antimicrobial Substances

Transformation of Helicobacter pylori into Coccoid Forms as a Challenge for Research Determining Activity of Antimicrobial Substances

Chilean Rhubarb, Gunnera tinctoria (Molina) Mirb. (Gunneraceae): UHPLC-ESI-Orbitrap-MS Profiling of Aqueous Extract and its Anti-Helicobacter pylori Activity

Pinocembrin: Insights into the Resources, Biosynthesis, Bioavailability, Safety, Biological Activities and Pharmacology

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