Evaluation of the immunomodulatory effect against splenocytes of Balb/c mice of biflorin obtained from Capraria biflora by a new isolation method

Silva, Mariza S. de Lima; Santos, Jefferson de A.; Alves, A. J.; Silva, Rosali M. F. da; Santos, Beate S.; Lorena, Virgínia Maria Barros de; Oliveira, Gibson Gomes de; Melo, Cristiane Moutinho Lagos de; Góes, Alexandre José da Silva

doi:10.1016/j.bjp.2019.01.010

Cited by 4 publications

(2 citation statements)

References 31 publications

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“…The target splenocytes were obtained from the mice, based on the procedure reported in a previous study [ 32 ]. The mice were cervical dislocated and followed by a soak (5 min) in 75% ethanol solution.…”

Section: Methodsmentioning

confidence: 99%

In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation

Lin

Guan

et al. 2021

Foods

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The soluble polysaccharides from a non-conventional and edible plant purslane (Portulaca oleracea L.), namely PSPO, were prepared by the water extraction and ethanol precipitation methods in this study. The obtained PSPO were selenylated using the Na2SeO3-HNO3 method to successfully prepare two selenylated products, namely SePSPO-1 and SePSPO-2, with different selenylation extents. The assay results confirmed that SePSPO-1 and SePSPO-2 had respective Se contents of 753.8 and 1325.1 mg/kg, while PSPO only contained Se element about 80.6 mg/kg. The results demonstrated that SePSPO-1 and SePSPO-2 had higher immune modulation than PSPO (p < 0.05), when using the two immune cells (murine splenocytes and RAW 264.7 macrophages) as two cell models. Specifically, SePSPO-1 and SePSPO-2 were more active than PSPO in the macrophages, resulting in higher cell proliferation, greater macrophage phagocytosis, and higher secretion of the immune-related three cytokines, including tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and IL-1β. Meanwhile, SePSPO-1 and SePSPO-2 were more potent than PSPO in the concanavalin A- or lipopolysaccharide-stimulated splenocytes in cell proliferation, or more able than PSPO in the splenocytes to promote interferon-γ secretion but suppress IL-4 secretion, or more capable of enhancing the ratio of T-helper (CD4+) cells to T-cytotoxic (CD8+) cells for the T lymphocytes than PSPO. Overall, the higher selenylation extent of the selenylated PSPO mostly caused higher immune modulation in the model cells, while a higher polysaccharide dose consistently led to the greater regulation effect. Thus, it is concluded that the employed chemical selenylation could be used in the chemical modification of purslane or other plant polysaccharides, when aiming to endow the polysaccharides with higher immuno-modulatory effect on the two immune cells.

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Section: Methodsmentioning

confidence: 99%

In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation

Lin

Guan

et al. 2021

Foods

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“…Active constituents of clove are eugenol, eugenin, kaempferol, rhamnetin, eugenitin, oleanolic acid, biflorin, and isobiflorin 11,12 . Biflorin promoted the production of immune stimulation in cytokines such as Interleukin-2, 6, 17, tumour necrosis factor-α, and interferons γ, inducing the immune profile toward a cytokines (Th1) response was able to stimulate mice splenocytes in albino, a laboratory-bred strain of the house mouse (Balb/c) 13 . The isolated phytocompounds eugenin, biflorin, and isobiflorin from the clove buds have proven anti-viral effects against the dengue viruses (DEN-V) by inhibiting the protease inhibitor 14 .…”

Section: Introductionmentioning

confidence: 99%

UFLC-MS Method Development of Vasicinone, Pellitorine, 6-Gingerol, Costunolide, Dehydrocostuslactone, Apigenin, and Validation of Piperine, Biflorin in Polyherbal Formulations

Jatoth,

Dhanabal,

Jeyprakash

et al. 2024

JNR

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Standardisation of polyherbal formulations is necessary for the quality, safety, quantity, and efficacy of botanicals in marketed and newly established formulations. The Ministry of Ayush, Govt of India, recommended using polyherbal formulations to treat COVID-19, i.e., Kabusura kudineer, Nilavembu kudineer, etc., Kabusura kudineer is a Siddha-based formulation. It prevents and treats COVID-19 due to some botanicals in Kabusura kudineer, which have proven anti-inflammatory, anti-viral, and immunomodulatory effects. The current work focuses on establishing a standard protocol for the Kabusura kudineer marketed, a novel dosage form called Hydaljss08, and in plant species, mainly present in both formulations. Both formulations contain some similar crude drugs and their active constituents. They are Zingiber officinale rhizome, Syzygium aromaticum flower buds, Adhatoda vasica leaves, Anacyclus pyrethrum roots, Saussurea lappa roots, Piper longum fruits, Clerodendrum serratum roots, Coleus amboinicus roots, contain active phytopharmaceuticals are 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, and apigenin. Existing liquid chromatography methods were reported for individual above active compounds, but not in these formulations and combined dosage forms. Working UFLC methods have not been reported individually nor combined for the Biflorin. The current study aims to develop UFLC methods for 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, and apigenin in polyherbal formulations Kabusura kudineer marketed, Hydaljss08 and in isolated, fractions, extract of plant species present in both dosage forms. The preliminary identification of the phytopharmaceuticals in the polyherbal formulations, isolated fractions, and extract of plant species was done by TLC and IR spectrum. The developed liquid chromatography method was novel, simple, linear, and rapid for estimating 6-gingerol, biflorin, vasicinone, pellitorine, costunolide, dehydrocostuslactone, piperine, apigenin in a plant species, and Ayush-based formulations.

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Biological evaluation of 1,3-benzodioxole acids points to 3,4-(methylenedioxy) cinnamic acid as a potential larvicide against Aedes aegypti (Diptera: Culicidae)

Silva,

Cruz

et al. 2024

Experimental Parasitology

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Evaluation of the immunomodulatory effect against splenocytes of Balb/c mice of biflorin obtained from Capraria biflora by a new isolation method

Cited by 4 publications

References 31 publications

In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation

In Vitro Immuno-Modulatory Potentials of Purslane (Portulaca oleracea L.) Polysaccharides with a Chemical Selenylation

UFLC-MS Method Development of Vasicinone, Pellitorine, 6-Gingerol, Costunolide, Dehydrocostuslactone, Apigenin, and Validation of Piperine, Biflorin in Polyherbal Formulations

Biological evaluation of 1,3-benzodioxole acids points to 3,4-(methylenedioxy) cinnamic acid as a potential larvicide against Aedes aegypti (Diptera: Culicidae)

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