HPLC-PDA method validated for the determination of hibalactone in Hydrocotyle umbellata subterraneous parts and its ultrasound-assisted extraction optimization

Oliveira, Matheus Gabriel de; Almeida, Paulo H.G. de; Oliveira, Thiago Levi Silva; Silva, Liliane de S.; Carvalho, Flávio Silva de; Alves, Suzana Ferreira; Borges, Leonardo Luiz; Santos, Pierre Alexandre dos; Silva, Vinicius B. da; Paula, José Realino de

doi:10.1016/j.bjp.2018.10.003

Cited by 14 publications

(6 citation statements)

References 29 publications

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“…The effect of this parameter was attributed to the possibility that the reduced mixture density attained as a result of the higher solvent-to-material ratio increased the ultrasound wave propagation speed while reducing the effect of attenuation of ultrasounds and increasing the transfer of energy/distance covered by wave/time [27,28]. Our findings were in agreement with those reported by Setyaningsih et al [29] for the extraction of polyphenols from rice grains, De Oliveira et al [30] for the extraction of hibalactone in Hydrocotyle umbellata subterraneous parts and Pavlic et al [31] for the extraction of polyphenols from peppermint.…”

Section: Influence Of Solid/liquid Ratiosupporting

confidence: 91%

Ultrasound-Assisted Extraction of Polyphenols from Crude Pollen

2020

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The aim of this study was to evaluate the extraction efficiency of polyphenols from crude pollen by an ultrasonic process. Prior to the polyphenols extraction, the crude pollen was defatted. The extraction from defatted pollen was carried out by varying four extraction parameters: ultrasonic amplitude (20%, 60% and 100%), solid/liquid ratio (10 g/L, 20 g/L and 30 g/L), temperature (35, 50 and 65 °C) and time (10, 20 and 30 min). The extracts were analyzed in terms of extraction yield (%), total phenolic content (TPC) and total flavones content (TFC). The extracted oil was analyzed in terms of fatty acids composition; myristic acid (159.1 µg × g−1) and cis-14-pentadecenoic acid (106.6 µg·g−1) were found in the highest amount in the pollen oil. The optimum conditions of extraction were determined and were, as follows: 100% amplitude of ultrasonic treatment, 30 g/L solid/liquid ratio, 40.85 °C and 14.30 min, which led to the extraction of 366.1 mg GAE/L of TPC and 592.2 mg QE/g of TFC, and also to an extraction yield of 1.92%.

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Section: Influence Of Solid/liquid Ratiosupporting

confidence: 91%

Ultrasound-Assisted Extraction of Polyphenols from Crude Pollen

2020

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“…9,59 The quality control and standardization of this compound from H. umbellata were then developed and validated using a HPLC-PDA method. 60 Therefore, HPLC can be effectively used to establish the difference between C. asiatica and H. umbellata. Our results revealed that both species are totally different in their phytochemical profiles which most possibly will contribute to pharmacological variance.…”

Section: Hplc Quantitative Analysismentioning

confidence: 99%

Centella asiatica (L.) Urb. and Hydrocotyle umbellata L. identification and quality assessment: A methodology comparison

Noppradit,

Klinnawee,

Leeratiwong

et al. 2024

Phytochemical Analysis

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IntroductionCentella is an important genus in the Apiaceae family. It includes Centella asiatica, which has significant edible and medicinal values. However, this species is easily confused due to its similar morphological traits to Hydrocotyle umbellata, hindering its utilization in the consumer and pharmacological industries.ObjectiveThe study aims to differentiate these two closely related plant species using reliable methods of confirming the authenticity of natural herbal medicines.MethodsOur work mainly focuses on the basic morphological characteristics, chemical markers, genetic fingerprints, and their biological responses.ResultsThe plants can be clearly differentiated using their leaf shapes, stipules, petioles, inflorescences, and fruit structures. Although the phytochemical compositions of the C. asiatica extract were similar to that of H. umbellata which included flavonoids, tannins, and saponins important to the plant's ability to reduce inflammation and promote healing of wounds, the H. umbellata extract showed significantly higher toxicity than that of C. asiatica. High‐performance liquid chromatography analysis was used to identify chemical fingerprints. The result revealed that C. asiatica had major triterpene glycoside constituents including asiaticoside, asiatic acid, madecassoside, and madecassic acid, which have a wide range of medicinal values. In contrast, triterpenoid saponins were not identified in H. umbellata. Furthermore, using SCoT1–6 primers was possible to effectively and sufficiently created a dendrogram which successfully identified the closeness of the plants and confirmed the differences between the two plant species.ConclusionTherefore, differentiation can be achieved through the combination of morphometrics, molecular bioactivity, and chemical analysis.

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“…Many studies have confirmed that high SMR (i.e., low medium density) significantly influences UAE efficiency positively (Dzah, Duan, Zhang, Golly, et al, 2020; Dzah, Duan, Zhang, Wen, et al, 2020; Maran et al, 2013; Shalmashi, 2009; Wang et al, 2013; Zhang et al, 2008; Zhao et al, 2007), supported by other studies summarized in Table 1. Attempting to explains the effect of SMR, some studies attribute increased yields obtained to the availability of solvent to dissolve solute until saturation (Oliveira et al, 2019; Pinela et al, 2019; Setyaningsih et al, 2019). Coussios et al (2007) related the density of extraction media to cavitation phenomenon, suggesting that higher cavitation occurs when extraction medium density is minimal (i.e., high SMR) (Coussios et al, 2007).…”

Section: Understanding the Superior Effects Of Smr On Uae Efficiencymentioning

confidence: 99%

Ultrasound assisted extraction: A relook at solvent to material ratio, its effects on process efficiency and how it can be exploited for different uses

Dzah

Dzigbor

2023

J Food Process Engineering

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Ultrasound assisted extraction (UAE) is one of the preferred green extraction techniques widely used for the extraction of bioactive compounds from plant materials. It has attracted much attention and many studies have been done to understand its mechanism, effects and efficiency. However, no study has discovered and given enough attention to the superior role solvent-to-material ratio (SMR) plays in moderating UAE efficiency. This study therefore emphasizes the importance of SMR as the main determinant of UAE efficiency and further proves its mechanistic effects with ultrasound physics. It discusses SMR effects on extraction medium density, acoustic energy transfer, cavitation rate, power intensity attenuation and free hydroxyl radical generation during water-based UAE. This understanding is needed to assist researchers in the design of their extraction experiments and optimizations. Without

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HPLC-PDA method validated for the determination of hibalactone in Hydrocotyle umbellata subterraneous parts and its ultrasound-assisted extraction optimization

Cited by 14 publications

References 29 publications

Ultrasound-Assisted Extraction of Polyphenols from Crude Pollen

Ultrasound-Assisted Extraction of Polyphenols from Crude Pollen

Centella asiatica (L.) Urb. and Hydrocotyle umbellata L. identification and quality assessment: A methodology comparison

Ultrasound assisted extraction: A relook at solvent to material ratio, its effects on process efficiency and how it can be exploited for different uses

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