Simultaneous liquid chromatography- tandem mass spectrometry method to quantify epicatechin and procyanidin B2 in rat plasma after oral administration of Trichilia catigua (catuaba) extract and its application to a pharmacokinetic study

Chavari, Mariana; Góes, Paulo Roberto Nunes de; Silva, Larissa Lachi; Barth, Anelise; Silva, André Oliveira Fernandes da; Longhini, Renata; Mello, João Carlos Palazzo de; Kimura, Elza; Diniz, Andréa

doi:10.1016/j.bjp.2018.08.011

Cited by 6 publications

(5 citation statements)

References 20 publications

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“…There are numerous bioanalytical techniques available that can be applied depending on the analytical goal, available resources and the scope of the study [ 13 ]. Some of the most commonly used sample preparation methods for the simultaneous purification of plasma and serum samples and the extraction of a broad spectrum of polar phenolic moieties include protein precipitation (PPT) [ 14 , 15 , 16 ], liquid–liquid extraction (LLE) [ 17 , 18 , 19 , 20 ], solid phase extraction (SPE) [ 21 , 22 , 23 ], enzymatic hydrolysis [ 24 , 25 , 26 ] and combinations of the aforementioned [ 27 , 28 ]. Interestingly, there have been some studies aptly questioning the suitability of enzymatic hydrolysis for the analysis of polar phenolics in biospecimen which we also took into account for the present work [ 24 , 29 ].…”

Section: Introductionmentioning

confidence: 99%

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

et al. 2022

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Analysis of plasma and serum provides valuable information on the amounts of polar phenols’ circulating after ingestion. In the present study, protein precipitation (PPT), liquid–liquid extraction (LLE), solid phase extraction (SPE), enzymatic hydrolysis and their combinations were meticulously evaluated for the extraction of a variety of polar phenolic moieties from plasma and serum. The recovery values of the above methods were compared; satisfactory recoveries (>60%) were attained for most analytes. Polar phenol aglycones undergo degradation with enzymatic hydrolysis; however, their extended phase II metabolism makes enzymatic hydrolysis a mandated process for their analysis in such biofluids. Hence, enzymatic hydrolysis followed by LLE was used for the identification of polar phenols in rats’ serum, after the long-term oral consumption of Corinthian Currant. Corinthian Currant is a Greek dried vine product rich in bioactive polar phenolics. Flavonoids and phenolic acids, detected as aglycones, ranged from 0.57 ± 0.08 to 181.66 ± 48.95 and 3.45 ± 1.20 to 897.81 ± 173.96 ng/mL, respectively. The majority of polar phenolics were present as phase II metabolites, representing their fasting state in the blood stream. This is the first study evaluating the presence of polar phenolics in the serum of rats following a long-term diet supplemented with Corinthian Currant as a whole food.

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Section: Introductionmentioning

confidence: 99%

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

et al. 2022

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“…A highly sensitive pharmacokinetic study of estrogens using a small microliter blood sample and UHPLC-MS/MS previously reported a LOD of 2.5 ng/mL [ 28 ]; Another study quantified epicatechin and procyanidin B2 in rat plasma after oral administration [ 14 ] and reported a lower LOQ of 5 ng/mL with a precision lower than 2% and a recovery higher than 90%. Finally, a pharmacokinetic study of rutin and quercetin in rats after oral administration has reported a LOQ 55.5 ng/mL [ 13 ].…”

Section: Resultsmentioning

confidence: 99%

“…Pharmacokinetic studies are currently an essential parameter for explaining the bioavailability and distribution of molecules with high pharmacological potential in crude extracts [ 13 ] or as isolated metabolites [ 14 , 15 , 16 ]. For all tests in preclinical studies, it is necessary to include these parameters.…”

Section: Introductionmentioning

confidence: 99%

“…Previous studies on the pharmacokinetics of polymethoxylated flavones [ 20 ], phenolic acids, and flavonoids [ 21 ] used HPLC-MS/MS to quantify rat plasma analytes. The other application, the HPLC-MS/MS in pharmacokinetic studies with high selectivity, is in organic crude extracts [ 13 , 14 , 15 ] due to the fact that it is only necessary to quantify one or a few flavonoids of the total molecules of the extract.…”

Section: Introductionmentioning

confidence: 99%

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Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

et al. 2020

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The purpose of this study was to develop, optimize, and fully validate a high-sensitivity methodology using UHPLC-MS/MS to simultaneously quantify hesperidin and naringenin in microsamples (100 µL) of murine plasma after intragastric administration of single pure flavonoids and a mixture. The optimization process allowed for high sensitivity with detection limits of approximately picogram order using an electrospray ionization (ESI) source in negative mode and an experiment based on multiple reaction monitoring (MRM). The validation parameters showed excellent linearity and detection limits, with a precision of less than 8% and a recovery of over 90%. This methodology was applied to compare the pharmacokinetic parameters for the administration of hesperidin and naringenin in individual form or in the form of a mixture. The results showed an absence of significant effects (p > 0.05) for Tmax and Cmax; however, the AUC presented significant differences (p < 0.05) for both flavonoids when administered as a mixture, showing an improved absorption ratio for both flavonoids.

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“…The development of a sensitive, specific, and validated method for detecting compounds in total blood or plasma is an important and limiting step in the process, especially for natural products such as crude extracts or isolated metabolites ( Ou-yang et al, 2013 ; Huang et al, 2017 ). Several studies have highlighted HPLC–MS/MS (high-performance liquid chromatography coupled with tandem mass spectrometry) for flavonoid pharmacokinetic determination because of its ability to perform a qualitative and quantitative analysis with high sensitivity and selectivity in different biological matrices, indicating also the importance of choosing the best extraction method since it discards any interference of matrix components ( Ou-yang et al, 2013 ; Huang et al, 2017 ; Attwa et al, 2018 ; Chavari et al, 2019 ; AlRabiah et al, 2020 ; Yuyama et al, 2020 ). Hesperidin, naringin, naringenin, quercetin, and luteolin are examples of flavonoids with pharmacokinetic properties that have been described by HPLC–MS/MS ( Xu et al, 2018 ; Araujo-León et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

Analysis of 2′-hydroxyflavanone (2HF) in mouse whole blood by HPLC–MS/MS for the determination of pharmacokinetic parameters

et al. 2023

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Given the lack of investments, structure, and difficulty of metabolite isolation, promising natural product studies do not progress to preclinical studies, such as pharmacokinetics. 2′-Hydroxyflavanone (2HF) is a flavonoid that has shown promising results in different types of cancer and leishmaniasis. For accurate quantification of 2HF in BALB/c mouse blood, a validated HPLC-MS/MS method was developed. Chromatographic analysis was performed using C18 (5μm, 150 mm × 4.6 mm). The mobile phase consisted of water containing 0.1% formic acid, acetonitrile, and methanol (35/52/13 v/v/v) at a flow rate and total running time of 0.8 mL/min and 5.50 min, respectively, with an injection volume of 20 µL. 2HF was detected by electrospray ionization in negative mode (ESI-) using multiple reaction monitoring (MRM). The validated bioanalytical method showed satisfactory selectivity without significant interference for the 2HF and IS. In addition, the concentration range between 1 and 250 ng/mL showed good linearity (r = 0.9969). The method showed satisfactory results for the matrix effect. Precision and accuracy intervals varied between 1.89% and 6.76% and 95.27% and 100.77%, respectively, fitting the criteria. No degradation of 2HF in the biological matrix was observed since stability under freezing and thawing conditions, short duration, postprocessing, and long duration showed deviations less than 15%. Once validated, the method was successfully applied in a 2HF oral pharmacokinetic study with mouse blood, and the pharmacokinetic parameters were determined. 2HF demonstrated a Cmax of 185.86 ng/mL, a Tmax of 5 min, and a half-life (T1/2) of 97.52 min.

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Simultaneous liquid chromatography- tandem mass spectrometry method to quantify epicatechin and procyanidin B2 in rat plasma after oral administration of Trichilia catigua (catuaba) extract and its application to a pharmacokinetic study

Cited by 6 publications

References 20 publications

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

Polar Phenol Detection in Plasma and Serum: Insights on Sample Pre-Treatment for LC/MS Analysis and Application on the Serum of Corinthian Currant-Fed Rats

Development and Optimization of a High Sensitivity LC-MS/MS Method for the Determination of Hesperidin and Naringenin in Rat Plasma: Pharmacokinetic Approach

Analysis of 2′-hydroxyflavanone (2HF) in mouse whole blood by HPLC–MS/MS for the determination of pharmacokinetic parameters

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