Phytochemical study of Harrisia adscendens

Santos, George Luís Dias dos; Lemos, Emerson Leite; Fernandes, Anna Flávia Costa; Rocha, Wilma Raianny Vieira da; Catão, Raïssa Mayer Ramalho; Braz-Filho, Raimundo; Tavares, Josean Fechine; Fechine, Yvana; Alves, Harley da Silva

doi:10.1016/j.bjp.2018.04.011

Cited by 2 publications

(1 citation statement)

References 12 publications

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“…The residue was discarded and the filtrate was subjected to several extractions with chloroform. The acidic aqueous phase was basified with NH 4 OH, stirred vigorously to pH 10, and then extracted with chloroform [27]. Subsequently, the chloroform fraction was evaporated under reduced pressure in a rotary evaporator at 40 • C, yielding 2.06 g of the TAF-Ap.…”

Section: Obtaining Of the Total Alkaloids Fraction From A Pyrifolium (Taf-ap)mentioning

confidence: 99%

Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from Aspidosperma pyrifolium Mart. (Apocynaceae)

Lins

Silva

Tavares

et al. 2021

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Aspidosperma pyrifolium is used in traditional medicine to treat inflammatory disorders. The aim of the study was to perform phytochemical characterization and evaluate the anti-inflammatory, anti-nociceptive and acute toxicity effects of the total alkaloid fraction (TAF-Ap) from stem barks. Two monoterpenic indole alkaloids were isolated by high performance liquid chromatography coupled with mass spectrometry (HPLC-MS) and the structural elucidation was performed using 1D and 2D NMR analysis. As for toxicity, no animals died at 50 mg kg−1 and this concentration presented mild sedation and forced breathing within the first 24 h. The lethal dose capable of killing 50% of the animals (LD50) was estimated to be 160 mg kg−1. In the pharmacological tests, the models used were 1% carrageenan-induced paw edema and peritonitis, 1% formalin-induced nociception and 1% acetic acid-induced abdominal writhing in Swiss mice. The study made it possible to isolate 15-methoxyaspidospermine and 15-methoxypyrifolidine, corroborating the results of pharmacological assays, which showed anti-inflammatory and analgesic potential, especially at 30 mg kg−1 (p < 0.001). Thus, the species was shown to be a promising source of active substances, with special attention paid to its toxicological potential.

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Section: Obtaining Of the Total Alkaloids Fraction From A Pyrifolium (Taf-ap)mentioning

confidence: 99%

Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from Aspidosperma pyrifolium Mart. (Apocynaceae)

Lins

Silva

Tavares

et al. 2021

Plants

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Phytochemical profile, toxicological evaluation of Rhipsalis baccifera (Sol.) Stearn (Cactaceae) extract and their antitumor activity in Ehrlich carcinoma-bearing mice

Cavalcantea¹,

Neto²,

Figueiredo³

et al. 2021

NRFHH

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Rhipsalis baccifera (Sol.) Stearn is a typical cactus from tropical regions with wide geographic distribution, and its therapeutic potential is not yet fully understood, such as antitumoral property. Thus, this study evaluated the cytotoxic ethanolic extract of R. baccifera (EERB) and its antitumor activity against Erlich's tumor in mice. The EERB was obtained, and its phytochemical profile was filed by thin-layer chromatography. The toxicity was evaluated in vitro and in vivo using the microcrustacean Artemia salina Leach and mice. The lethal dose was determined after implantation of a tumor cell suspension, with subsequent treatment with EERB (200 mg/kg and 100 mg/kg) 48h after implantation. These values represent the tenth part of the DL50 and CL50, respectively. The presence of phenols, tannins and triterpenes were demonstrated in the phytochemical results. Toxicity was dose-dependent, and the tumor inhibition was 84.1% and 75.8% at doses of 200 mg/kg and 100 mg/kg, respectively. We can highlight that the growth of Erlich's carcinoma suffered inhibitory effects against the EERB. EERB was found to have low acute toxicity and a high potential for use in antitumor therapy. Thus, new studies involving pre-clinical and clinical analyses of the extract are essential to determine the safe dose.

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Phytochemical study of Harrisia adscendens

Cited by 2 publications

References 12 publications

Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from Aspidosperma pyrifolium Mart. (Apocynaceae)

Bioguided Isolation of Alkaloids and Pharmacological Effects of the Total Alkaloid Fraction from Aspidosperma pyrifolium Mart. (Apocynaceae)

Phytochemical profile, toxicological evaluation of <i>Rhipsalis baccifera</i> (Sol.) Stearn (Cactaceae) extract and their antitumor activity in Ehrlich carcinoma-bearing mice

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