Antioxidant activity evaluation of dried herbal extracts: an electroanalytical approach

Eugenia dysenterica ex DC Mart. (Myrtaceae), popularly known as “cagaita,” is a Brazilian plant rich in polyphenols and other antioxidant compounds. Aiming to evaluate the potential use of cagaita in pathologies involving oxidative stress, such as neurodegenerative disorders, this study investigated its antioxidant potential and neuroprotective effect. Electrochemical approaches and aluminium-induced neurotoxicity were used to determine respectively in vitro and in vivo antioxidant properties of cagaita. Voltammetric experiments were carried out in a three-electrode system, whose working electrode consisted of glassy carbon. Male Swiss mice were administered with AlCl3 orally at a dose of 100 mg/kg/day and with cagaita leaf hydroalcoholic extract (CHE) at doses of 10, 100, and 300 mg/kg/day. The redox behavior of CHE presented similar features to that of quercetin, a widely known antioxidant standard. CHE prevented mouse memory impairment which resulted from aluminium intake. In addition, biochemical markers of oxidative stress (catalase, superoxide dismutase activity, and lipid peroxidation) were normalized by CHE treatment. The potential of CHE to prevent aluminium-induced neurotoxicity was reflected at the microscopic level, through the decrease of the number of eosinophilic necrosis phenotypes seen in treated groups. Moreover, the protective effect of CHE was similar to that of quercetin, which was taken as the standard. These findings showed that the CHE of cagaita leaves has a potential to protect the brain against oxidative-induced brain damage.

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“…As all polyphenols, quercetin promotes ROS scavenging, which minimizes oxidative stress and oxygen-related injuries in brain mater [12–21]. …”

Section: Resultsmentioning

confidence: 99%

“…Through such methods is possible to understand the redox features of a pool of compounds and provide important information about the thermodynamical feasibility of ROS reduction [11, 12]. …”

Section: Introductionmentioning

confidence: 99%

Antioxidant and Neuroprotective Properties of Eugenia dysenterica Leaves

Thomaz

Peixoto

Oliveira

et al. 2018

Oxidative Medicine and Cellular Longevity

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“…The determination of free radical scavenging capacity was conducted according to previous outreaches (Leite et al, 2018). The antioxidant activity (AOA) in terms of % discoloration was calculated based on the original color intensity of pure DPPH solution.…”

Section: Antioxidant Activity Assessmentmentioning

confidence: 99%

“…In order to correlate results, minimize dimensions basing on variance/correlation matrix and stablish a statistical model capable of explaining the findings, PCA was used (Leite et al, 2018). All data were considered to stablish an appropriate decision tree.…”

Section: Multivariate Statistical Analysismentioning

confidence: 99%

Correlation of polyphenol content and antioxidant capacity of selected teas and tisanes from Brazilian market

Alves

Rodrigues

Thomaz

et al. 2020

Braz. J. Food Technol.

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In this work, it was evaluated the phenolic content, redox behavior and antioxidant capacity of several selected teas and tisanes from Brazilian market. The samples were classified as simple (single herb) or composed (blend of two or more herbs). In addition, complementary multivariate statistical approaches were used to identify the correlation and interdependence between the amount of major phytocomponents, such as phenols and flavonoids, as well as the antioxidant activity of the products. Results showed that the total polyphenol and total flavonoid concentrations are correlated to thermodynamic feasibility of reactive oxygen species reduction. The statistical modeling differentiated the results datasets in principal components, whose flavonoid content presented itself as the main parameter to segregate data between simple and composed products. On the other hand, the whole polyphenol content, in both teas and tisanes, was more relevant regarding their antioxidant capacity than the flavonoids content. Considering that the manufactures do not display the real amount of each herb in the labels of the composed products, it was not possible to confirm whether the blends of several species lead to a substantial enhancement on their antioxidant capacity. Furthermore, the redox profile and overall polyphenol content suggest that the consumption of green and black teas as well as traditional yerba mate may be worthwhile in preventing ailments associated with oxidative stress in Brazilian population. Notwithstanding, further clinical studies are required to validate this hypothesis.

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“…The antioxidant capacity of phytochemicals is a well-known feature, and its mechanisms are nonetheless explored in many cosmetical and nutraceutical products [9,10]. Given the relationship between the chemical structure and physicochemical features such as antioxidant capacity, many compounds bearing similar structural characteristics also showcase similar thermodynamic feasibility to undergo oxidation [11][12][13].…”

Section: The Physicochemical Aspects Of Phytochemical Antioxidantsmentioning

confidence: 99%

How Phytocomponents May be Valuable Against Oxidative Stress in Brain Tissue?

Thomaz¹

2020

GDT

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This short commentary is intended to briefly discuss the benefits of plant secondary metabolites against brain tissue injury promoted by oxidative stress. Henceforth, the physicochemical features and biological activities of phytocomponents were assessed based on literature reports, which showcased their thermodynamic feasibility to reduce reactive oxygen species. It was strongly hinted that secondary metabolites possessing antioxidant potential may be of use in the therapeutics against neurodegenerative disorders owing to free radical scavenging, what hinders further damage to brain tissue.

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Antioxidant activity evaluation of dried herbal extracts: an electroanalytical approach

Cited by 50 publications

References 32 publications

Antioxidant and Neuroprotective Properties of Eugenia dysenterica Leaves

Antioxidant and Neuroprotective Properties of Eugenia dysenterica Leaves

Correlation of polyphenol content and antioxidant capacity of selected teas and tisanes from Brazilian market

How Phytocomponents May be Valuable Against Oxidative Stress in Brain Tissue?

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