Optimization of baicalin water extraction process from Scutellaria baicalensis (a traditional Chinese medicine) by using orthogonal test and HPLC

Ni, Huilin; Wu, Zhiyong; Ishfaq, Muhammad; Lu, Zhaoqing; Li, Jichang

doi:10.1016/j.bjp.2018.02.001

Cited by 13 publications

(6 citation statements)

References 9 publications

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“…According to the total flavonoid content, the extraction temperature has a small effect. Ni et al [32] optimized the decoction condition of baicalin from Scutellaria baicalensis roots. Here, the extraction time was varied from 30 to 150 min.…”

Section: Resultsmentioning

confidence: 99%

Optimization of dynamic maceration of Clausena anisata (Willd.) Hook. f. ex Benth. leaves to maximize trans-anethole content

et al. 2021

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Trans-anethole possesses several biological and pharmacological effects. It is also used as masking agent in household products and as a flavoring agent in food. Clausena anisata (Willd.) Hook. f. ex Benth. leaves are reported as a source of trans-anethole that makes their anise-like odor. This work sought to optimize the dynamic maceration of C. anisata leaves to maximize the trans-anethole content. The circumscribed central composite experimental design was applied to investigate the effects of extraction temperature and time on extraction yield, trans-anethole content in the extract, and trans-anethole content in the plant raw material. The results showed that the extraction yield was high when C. anisata leaves were extracted over a long extraction time, while the extraction temperature had a lesser effect on the extraction yield. Trans-anethole content in the extract and plant raw material determined using a linear, specific, precise, and accurate HPLC method was high when a medium extraction temperature with a short extraction time was used. The optimal condition that maximized trans-anethole content involved an extraction temperature of 61.8 °C and an extraction time of 12.9 min, respectively. The percentage error of the prediction conducted by computer software was low, suggesting that the prediction was highly accurate. In conclusion, the optimal condition of dynamic maceration obtained from this work could be used as a guide for maximizing trans-anethole content from C. anisata leaves.

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Section: Resultsmentioning

confidence: 99%

Optimization of dynamic maceration of Clausena anisata (Willd.) Hook. f. ex Benth. leaves to maximize trans-anethole content

et al. 2021

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“…It is often necessary to examine multiple levels of multiple factors for optimization of an extraction process. In order to reduce the number of trials and to investigate the interaction between factors, uniform design and orthogonal design methods are often used in China to design experiments [21]. It is worthy to mention that linear model is mostly used in the extraction process.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

Rui

Sheng

et al. 2019

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In this study, a simultaneous water extraction process for baicalin, wogonoside, and chlorogenic acid has been optimized. The effect of extraction temperature, extraction time, and liquid–solid ratio was scrutinized by single factor experiments and further analyzed by Box–Behnken design (BBD) approach using response surface methodology (RSM). The extraction yield of investigated compounds was determined by high performance liquid chromatography (HPLC). Single-factor experiments and response surface analysis results revealed that the optimized conditions are: Liquid to solid ratio 25:1 (mL/g), extraction temperature 93 °C, extraction time 2.4 h, and the extraction cycle two. Importantly, it has been noted that under the above conditions, concentrations of baicalin, wogonoside, and chlorogenic were 0.078, 0.031, and 0.013 mg/mL, respectively, and the overall desirability (OD) value was 0.76 which was higher than the non-optimized conditions and the deviation from the predicted OD value was only 2.44%. Conclusively, it has been suggested that the model was stable and feasible, and fit for extraction of baicalin, wogonoside, and chlorogenic acid from Scutellariae Radix and Lonicerae (L.) japonicae Flos.

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“…Baicalin was prepared in our laboratory according to a procedure reported in a previous study (Ni, Wu, Muhammad, Lu, & Li, 2018). Briefly, air‐dried and powdered herbs of S. baicalensis were exhaustively extracted with 60% ethanol at room temperature by percolation.…”

Section: Methodsmentioning

confidence: 99%

Baicalin protects giant freshwater prawn, Macrobrachium rosenbergii against Vibrio parahaemolyticus via modulation of the Toll signaling pathway

Zhang

Shen

et al. 2021

J World Aquaculture Soc

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To evaluate the protective effect of baicalin against a Vibrio parahaemolyticus challenge, immune parameters were analyzed and RNA interference (RNAi) was performed in the present study. The results from challenge tests showed that the cumulative mortalities in the 200 and 400 mg/kg baicalin groups after challenge with V. parahaemolyticus were significantly lower than they were in the 100 mg/kg baicalin or control groups (p < .05); the relative percent of survival reached 71.4%. Macrobrachium rosenbergii injected with V. parahaemolyticus exhibited a significant increase in lysozyme (LZM) and inducible nitric oxide synthase levels as well as phagocytic activity because of the immune response, while after treatment with baicalin, these immune parameters were further enhanced. The gene expression levels of anti‐lipopolysaccharide factors (ALFs), LZM, relish, and crustin were increased after treatment with baicalin or challenge with V. parahaemolyticus. The results of RNAi experiments demonstrated that the expression of crustins (Cru2, Cru4, Cru5, and Cru8), ALFs (ALF1, ALF3, ALF4, and ALF5), lysozymes (Lys1 and Lys2), and relish were significantly elevated after V. parahaemolyticus challenge. After MrToll4 was knocked down by siRNA, the expression levels of antimicrobial peptides (AMPs), except relish and Cru4, were inhibited. However, after oral administration of 400 mg/kg baicalin, the expression levels of all these AMPs, except Cru2, Cru4, ALF3, and relish, were upregulated.

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Optimization of baicalin water extraction process from Scutellaria baicalensis (a traditional Chinese medicine) by using orthogonal test and HPLC

Cited by 13 publications

References 9 publications

Optimization of dynamic maceration of Clausena anisata (Willd.) Hook. f. ex Benth. leaves to maximize trans-anethole content

Optimization of dynamic maceration of Clausena anisata (Willd.) Hook. f. ex Benth. leaves to maximize trans-anethole content

Optimization of Baicalin, Wogonoside, and Chlorogenic Acid Water Extraction Process from the Roots of Scutellariae Radix and Lonicerae japonicae Flos Using Response Surface Methodology (RSM)

Baicalin protects giant freshwater prawn, Macrobrachium rosenbergii against Vibrio parahaemolyticus via modulation of the Toll signaling pathway

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