Comparison of bioactive compounds content in leaf extracts of Passiflora incarnata , P. caerulea and P. alata and in vitro cytotoxic potential on leukemia cell lines

Ożarowski, Marcin; Piasecka, Anna; Paszel-Jaworska, Anna; Chaves, Douglas Siqueira de Almeida; Romaniuk, Aleksandra; Rybczyńska, Maria; Gryszczyńska, Agnieszka; Sawikowska, Aneta; Kachlicki, P.; Mikołajczak, Przemysław Ł.; Seremak‐Mrozikiewicz, Agnieszka; Klejewski, Andrzej; Thiem, Barbara

doi:10.1016/j.bjp.2018.01.006

Cited by 55 publications

(29 citation statements)

References 36 publications

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“…Plants were identified at the Department of Medicinal and Cosmetic Natural Products, Poznań University of Medical Sciences, and also by Jorge Ochoa from the Passiflora Society International and the Department of Horticulture, Long Beach City College (California, USA). The voucher specimens have been deposited in the Herbarium of the Institute of Natural Fibers and Medicinal Plants in Poznań, Poland [37]. Evaluation of biological activity of leaf extracts in range of their antioxidant assessment is a continuation of previous examinations of extracts that have been phytochemically characterized and described by Ożarowski et al [37].…”

Section: Plant Materialsmentioning

confidence: 97%

“…Mature and healthy leaves of P. incarnata, P. alata and P. caerulea were obtained from plants growing in the greenhouse of the Poznań University of Medical Sciences, Poland, in controlled conditions as follows: temperature range from 25 º C to 40 º C, humidity 60-70%, as described previously [37]. Plants were identified at the Department of Medicinal and Cosmetic Natural Products, Poznań University of Medical Sciences, and also by Jorge Ochoa from the Passiflora Society International and the Department of Horticulture, Long Beach City College (California, USA).…”

Section: Plant Materialsmentioning

confidence: 99%

“…The voucher specimens have been deposited in the Herbarium of the Institute of Natural Fibers and Medicinal Plants in Poznań, Poland [37]. Evaluation of biological activity of leaf extracts in range of their antioxidant assessment is a continuation of previous examinations of extracts that have been phytochemically characterized and described by Ożarowski et al [37]. Thus, procedures such as conventional preparation of methanolic extracts from 5 g of leaves of P. alata, of P. caerulea, of P. incarnata; determination of total phenolic content with Folin-Ciocalteu reagent expressed as gallic acid; LC phytochemical profile comparison of extracts using HPLC-DAD-MS n and UPLC-MS/MS methods and results were described previously [37], however, results of these studies were also discussed along with antioxidant capacity of extracts.…”

Section: Plant Materialsmentioning

confidence: 99%

“…In recent years, attention has been paid to the biological activity of phytochemically defined extracts from different species of Passiflora which can be obtained not only from natural habitats of South America (mainly Colombia and Brazil), but also from controlled greenhouse cultivation in various countries or using biotechnological methods such as micropropagation in sterile and monitored in vitro laboratory conditions [36][37][38][39][40][41][42][43].…”

Section: Introductionmentioning

confidence: 99%

“…The aim of the current study was to investigate antioxidant activity using three common in vitro methods presented as DPPH, ABTS and FRAP of the crude methanolic extracts from leaves of most popular species such as Passiflora incarnata L., Passiflora caerulea L. and Passiflora alata Curtis cultivated in Poland. Results of studies were compared with those of phenolic compounds occurring in extracts, according to papers by Ożarowski et al [37] and Hadaś et al [43].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of in vitro antioxidative activities of crude methanolic extracts of three species of Passiflora from greenhouse using DPPH, ABTS and FRAP methods

et al. 2019

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Summary Introduction:. It is well documented that many species from Passifloraceae family can provide edible and nutritious fruits while the leaves of cultivated plants are renewable and waste material. This biomass may be further used in various sectors, especially as a bioactive food additive and as source of innovative pharmaceuticals, cosmetics or feed additives. The biomaterials and green chemistry are new sectors bioeconomy according to the high-level horizontal strategies and bio-based industries in Europe. In recent years, attention has been paid to the biological activity and phytochemical profiles of extracts from different species of Passiflora. However, there is little comparative studies using the same procedures and techniques in the same laboratory conditions for study of plant material obtained from the similar greenhouse conditions. Objective: This study was focused on the examination of antioxidative activities of low concentrations of crude extracts from leaves of Passiflora incarnata L., Passiflora caerulea L., and Passiflora alata Curtis. Methods: The activity was evaluated using 2,2-diphenyl-1-picrylhydrazyl (DPPH), 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS) radical scavenging and ferric reducing antioxidant power (FRAP) methods. Results of study were supported by estimation of chemical composition with secondary metabolites profiling in extracts which were carried out previously for the same extracts from three Passiflora species. One-way ANOVA analysis revealed significant differences in the antioxidant activity of various concentrations of the extracts using the DPPH and ABTS radical models, and FRAP method. Results: Measurement of antioxidant capacity (expressed as trolox equivalent, TE) showed that the most active was extract of P. caerulea > P. alata > P. incarnata. Phytochemical analysis for extracts of P. caerulea and P. incarnata showed greater similarities in metabolites content than P. alata. However, comparative statistical analysis of antioxidant activity showed that despite this phytochemical similarities, extract from P. alata leaves had higher activities than extract from leaves P. incarnata. Antioxidant effect of extract from P. alata can be explain by terpenoids presented in this extract. In this work, there have been discussed activities against Acanthamoeba castellanii strain, antibacterial and antifungal activities against selected clinical microorganisms (Enterococcus faecalis, Escherichia coli, Staphylococcus aureus, and Candida albicans, Micro-sporum gypseum), and anti-leukemic activities tested in human acute lymphoblastic leukemia cell lines for this extracts, which have been described in previous authors’ publications. Conclusion: Our current and previous studies showed that the same crude extracts from leaves of P. alata, P. caerulea, P. incarnata exerted not only antioxidant potential in vitro but also few interesting properties such as antibacterial, antifungal, amoebostatic, amoebicidal activities, which indicate the possibility of using these extracts in both a healthy diet and natural cosmetics. Leaves of this species may become an interesting source of biomaterials which can exert health-promoting effects.

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Section: Plant Materialsmentioning

confidence: 97%

Section: Plant Materialsmentioning

confidence: 99%