Isolation and identification of cytotoxic compounds from a fruticose lichen Roccella montagnei , and it's in silico docking study against CDK-10

Mishra, Tripti; Shukla, Shipra; Meena, Sanjeev; Singh, Ruchi; Pal, Mahesh; Upreti, D. K.; Datta, Dipak

doi:10.1016/j.bjp.2017.07.006

Cited by 15 publications

(6 citation statements)

References 9 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Furthermore, Mallavadhani and Sudhakar reported roccellatol along with nine known metabolites from R. montagnei [14]. In recent times, isomer of methylγ-orsellinate and roccellatol which were isolated from R. montagnei were screened for radical scavenging potential, anti-inflammatory and anti-arthritic activities [15], as well as, isolation of cytotoxic compounds along with docking evidences has been reported by Mishra's group [16]. Earlier, our group has established and reported the phytochemical analysis, antimicrobial, antimycobacterial, antioxidant and cytotoxicity activities of various extracts of R. montagnei [4].…”

Section: Introductionmentioning

confidence: 90%

Chemical and pharmacological evaluation of manglicolous lichen Roccella montagnei Bel em. D. D. Awasthi

2019

View full text Add to dashboard Cite

Background: Lichen is a composite organism composed of fungus in association with algae or cyanobacteria. Particularly, lichen betide to mangroves are named as manglicolous lichens. From the folklore, lichen extracts were used in the management of many infections and diseases. Results: The chemical investigation of acetone extract of manglicolous lichen Roccella montagnei (RM-Ac) yielded nine known metabolites namely divarinolmonomethylether (1), ethyl divaricatinate (2), divarinol (3), orcinol (4), methyl 2,6-dihydroxy-4-methylbenzoate (5), haematommic acid (6), atranol (7), ethyl haematommate (8) and ethyl orsellinate (9). Except 4, all are for the first reported from this species. The RM-Ac and its metabolites (1-9) were screened for antioxidant (DPPH, ABTS and superoxide free radical assays), anti-inflammatory, anticancer (SRB assay using A549, DLD-1, FADU, HeLa and MCF-7) and acute toxicity studies. The pharmacological results showed that compounds 6 and 8 depicted potent inhibitory profile against ABTS free radical (with IC 50 value of 40.0 and 40.5 μg/mL, respectively) and protein denaturation (with IC 50 value of 435 and 403 μg/mL, respectively). LD 50 of RM-Ac was found to be above 2 g/Kg body weight. Moreover, the RM-Ac showed prominent inhibition of formalin-hind albino rat paw oedema at both the tested doses, i.e., 100 and 200 mg/Kg b.w than that of the standard drug (indomethacin). Furthermore, the compounds 6 and 8 exhibited significant degree of specificity against HeLa, FADU and A549; besides, they showed very little degree of specificity against NHME cell line specifying less toxicity to normal cells. Conclusion: It can be concluded that the manglicolous lichen R. montagnei has an aptitude to act against free radicals, inflammation and cancer, and the main metabolites responsible for its biological activity are 6 and 8.

show abstract

Section: Introductionmentioning

confidence: 90%

Chemical and pharmacological evaluation of manglicolous lichen Roccella montagnei Bel em. D. D. Awasthi

2019

View full text Add to dashboard Cite

show abstract

“…The previously chemical investigations on this species showed the presence of depside, depsidone, quinone, sterol, usnic acid derivatives, and phenolic compounds [3][4][5] . The bioactive assessments on extracts and isolated compounds from this species revealed antiinflammatory, antimicrobial, anti-arthritic, antioxidant, and anticancer activities 2,4,6,7 . The phytochemical investigation on this species was further studied on n-hexane extract led to the isolation and structural elucidation of 5 compounds.…”

Section: Introductionmentioning

confidence: 94%

The phytochemical investigation from n-hexane extract of the lichen Roccella montagnei

Nguyen

Huy

2021

Sci. Tech. Dev. J.

View full text Add to dashboard Cite

Introduction: R occella montagnei is widely distributed in subtropical regions. As the continuous study on the hexane extract of Roccella montagnei lichen, the isolation and structural determination of five compounds were addressed. Method: The crude extract was obtained from the dried lichen powder's extraction at room temperature. The n-hexane, n-hexane-ethyl acetate, and ethyl acetate extracts were obtained by the liquid-liquid partition method. The organic compounds were isolated from n-hexane extract by silica gel and Sephadex LH-20 column chromatography. Their chemical structures were identified by the NMR and HR-ESI-MS data analysis and the comparison of their NMR data with the published data. Results: Five compounds were isolated and chemically structural identified, consisting of 3b -hydroxy-7a-methoxystigmast-5-ene (1), sekikaic acid (2), lichenxanthone (3), (+)-6,8-dihydroxy-3-propyl-3,4-dihydroisocoumarin (4), and ar-turmerone (5). Conclusion: To the best of our knowledge, except 3 which was reported from this species for the first time, four isolated compounds left did not known to be present in Roccella genus before.

show abstract

“…These novel antibacterial compounds have potentiality to not only kill the bacteria but also can be used as an effective drug loader or carrier 25,26,27,28,29 .…”

Section: Materials and Methods: A Collection Of Lichens And Extractimentioning

confidence: 99%

Untitled

2018

IJPSR

View full text Add to dashboard Cite

ABSTRACT:Lichens came as a consortium of two distinct and dissimilar components-the mycobiont and the phycobionts, which were defined as a composite organism. Lichen vegetation in India is dominated by more than 2500 species. Lichen secondary metabolites specially the phenolics, depsides and depsidones are unique in their origin and are found nowhere else. Much attention has been paid on the biological roles of lichen secondary substances in the recent past; which have been found to possess a lot of bioactivities, such as anti-tumor, antibacterial, antifungal, antiviral, anti-inflammatory and antioxidant activities. The present study aims on the bactericidal activity of lichen, Cladonia scabriuscula, collected from the Gopeshwar, Uttarakhand in the Western Himalayas. The 50% v/v ethanolic extract (CSE) extracted through cold extraction method was tested for its bactericidal potential against prevalent water borne pathogen and its antioxidant potential through CLSI protocol. The CSE was found active against E. coli and S. typhimurium with MIC 2.48 and 1.02 mg/ml respectively; while the FRSA IC 50 was 0.625 mg/ml.

show abstract

Isolation and identification of cytotoxic compounds from a fruticose lichen Roccella montagnei , and it's in silico docking study against CDK-10

Cited by 15 publications

References 9 publications

Chemical and pharmacological evaluation of manglicolous lichen Roccella montagnei Bel em. D. D. Awasthi

Chemical and pharmacological evaluation of manglicolous lichen Roccella montagnei Bel em. D. D. Awasthi

The phytochemical investigation from n-hexane extract of the lichen Roccella montagnei

Untitled

Contact Info

Product

Resources

About