Chemical identification and quantification of Hu-Gu capsule by UHPLC–Q-TOF-MS and HPLC-DAD

Ren, Qiang; Long, Shi-song

doi:10.1016/j.bjp.2017.06.002

Cited by 5 publications

(2 citation statements)

References 14 publications

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“…A base peak at m / z 531.18730 was given by the MS 2 spectrum, which corresponded to hydroicaritin aglycone bearing an O-linked monosaccharide substituent. Based on the authenticated standard and a previous report [ 38 ], it was concluded that compound 26 was icariin.…”

Section: Resultsmentioning

confidence: 93%

Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC‐DAD‐Quadrupole‐Orbitrap‐MS and the Antioxidant Activity In Vitro

Reheman

Aisa

Ling

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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By merging a high-performance liquid chromatography diode array detector (HPLC-DAD) method with high-performance thin-layer chromatography (HPTLC), an assay was developed for chemical fingerprinting and quantitative analysis of traditional medicine Majun Mupakhi ELA (MME), and constituent compounds were identified using HPLC coupled with UHPLC-DAD-Quadrupole-Orbitrap-MS method. In addition, the antioxidant capacity of MME was assessed based on the ability of components to scavenge radicals using in vitro method. Using a HPLC-DAD method with HPTLC easily validated the chemical fingerprinting results and quantified three characteristic components, namely, gallic acid (1), daidzein (2), and icariin (3), in commercial MMEs. The three compounds presented excellent regression values (R2 = 0.9999) in the ranges of the test and the method recovery was in the range from 100.49% to 100.68%. The fingerprints had 27 common characteristic peaks, of which 13 were verified by rapid UHPLC-DAD-Q-Orbitrap-MS analysis. In vitro antioxidant assays rapidly assessed and contrasted antioxidant activity or the free radical scavenging activity of the main polyphenolic classes in MMEs, and the antioxidant capacity was mostly affected by the presence of gallic acid. Thus, this study establishes a powerful and meaningful approach for MME quality control and for assessing in vitro antioxidant activity.

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Section: Resultsmentioning

confidence: 93%

Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC‐DAD‐Quadrupole‐Orbitrap‐MS and the Antioxidant Activity In Vitro

Reheman

Aisa

Ling

et al. 2018

Evidence-Based Complementary and Alternative Medicine

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show abstract

“…Q‐TOF MS E , a high‐resolution tandem spectrometry analytical method, can provide high sensitivity, high accuracy and precision of mass spectra. Furthermore, it is possible to identify the components by analysis of their fragmentation ions generated by collision‐induced dissociation (Jeong & Lim, 2018; Ren & Long, 2017).…”

Section: Introductionmentioning

confidence: 99%

Enrichment of flavonoid‐rich extract from Bidens bipinnata L. by macroporous resin using response surface methodology, UHPLC–Q‐TOF MS/MS‐assisted characterization and comprehensive evaluation of its bioactivities by analytical hierarchy process

Yang

Bai

Zhang³

et al. 2020

Biomedical Chromatography

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The extract of Bidens bipinnata L. exhibited wide spectrum of bioactivities owing to the presence of flavonoids. In this study, a purification process was developed to enrich the flavonoid-rich extract from B. bipinnata L. (BBTF). AB-8 resin was selected for the purification of total flavonoids. Response surface methodology coupled with Box-Behnken design was employed to optimize the purification condition; it was optimized as pH 5.1, volume of ethanol 80 ml, flow rate of ethanol 1.8 bed volume per hour (BV/h) and concentration of ethanol 76.0%. The total flavonoid content of BBTF was 56.48% under the optimal conditions. The identification of flavonoids in BBTF was conducted using UHPLC-ESI-Q-TOF MS. A total of 14 compounds, including 12 flavonoids, were identified in BBTF. Finally, the in vitro antioxidant activities, α-glucosidase and α-amylase inhibitory activities of BBTF were comprehensively analyzed by an analytical hierarchy process. The results indicated that it exhibited higher bioactivities than the crude extract. These findings suggested that the optimized process could significantly enhance the purity of flavonoids and their bioactivities. This study showed a comprehensive analysis of a total flavonoid extract of B. bipinnata L. for the first time, which could provide a useful approach for its purification process and quality control as well as bioactivities.

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A simple and selective LC‐MS/MS method for quantification of ikarisoside A in rat plasma and its application to a pharmacokinetic study

Wang

Zhang

et al. 2018

Biomedical Chromatography

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Ikarisoside A is a natural flavonoid isolated from Epimedium plants. To further evaluate its medicinal potential, a sensitive and robust LC-MS/MS method was developed and validated for the assay of ikarisoside A in rat plasma. Orientin was used as an internal standard. The electrospray ionization was operated in its negative ion mode while ikarisoside A and IS were measured by selected reaction monitoring using precursor-to-product ion transitions of m/z 499.1 → 353.0 and m/z 446.9 → 327.6, respectively. This LC-MS/MS method had good sensitivity (LLOQ = 1.5 ng/mL), accuracy (both intra- and inter-day RE ≤ ±11.9%) and precision (both intra- and inter-day RSD ≤8.5%). The pharmacokinetics of ikarisoside A was subsequently profiled in Sprague-Dawley rats. Following oral administration (35 mg/kg), ikarisoside A reached maximum plasma concentration (C , 207.6 ± 96.7 ng/mL) attained at 1.10 ± 0.42 h. Following oral administration, the clearance and terminal half-life were 42.9 ± 26.5 L/h/kg and 3.15 ± 0.80 h by oral route, respectively.

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Chemical identification and quantification of Hu-Gu capsule by UHPLC–Q-TOF-MS and HPLC-DAD

Cited by 5 publications

References 14 publications

Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC‐DAD‐Quadrupole‐Orbitrap‐MS and the Antioxidant Activity In Vitro

Quality Evaluation of the Traditional Medicine Majun Mupakhi ELA via Chromatographic Fingerprinting Coupled with UHPLC‐DAD‐Quadrupole‐Orbitrap‐MS and the Antioxidant Activity In Vitro

Enrichment of flavonoid‐rich extract from Bidens bipinnata L. by macroporous resin using response surface methodology, UHPLC–Q‐TOF MS/MS‐assisted characterization and comprehensive evaluation of its bioactivities by analytical hierarchy process

A simple and selective LC‐MS/MS method for quantification of ikarisoside A in rat plasma and its application to a pharmacokinetic study

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