Short-term carcinogenesis evaluation of Casearia sylvestris

Tirloni, Cleide A.S.; Traesel, Giseli Karenina; Lívero, Francislaine Aparecida dos Reis; Neto, Salvador Dias Vieira; Junior, Ronaldo de Faria; Paim, Thaís Casagrande; Santos, Joyce Alencar; Oesterreich, Silvia Aparecida; Santos, Ariany Carvalho dos; Souza, Roosevelt Isaías Carvalho; Cardozo, Euclides Lara; Gasparotto, Arquimedes

doi:10.1016/j.bjp.2017.05.009

Cited by 1 publication

(2 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…but also for assessment of the effects of plants (especially medically important plants) on other organisms. For example, Tirloni et al [47] studied the effects of Casearia sylvestris in Wistar rats by the MN test, comet assay and tumour marker analyses. They concluded that this plant did not cause any mutagenic and genotoxic effects or any alterations in important tumour markers.…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Short-term mutagenicity test by using IRAP molecular marker in rice grown under herbicide treatment

Yılmaz

Marakli

Yüzbaşioğlu

et al. 2018

Biotechnology & Biotechnological Equipment

View full text Add to dashboard Cite

Rice is an economically important plant as well as a model organism. The rice genome consists of 35% retrotransposons. Although most of the retrotransposons are inactivated through evolutionary processes, they can be activated under various biotic and abiotic stress conditions. The main objective of this study was to explore the effects of herbicides on retrotransposon activities and the usage of retrotransposons in short-term mutagenicity tests. In this study, bentazone and an MCPA (2-methyl-4-chlorophenoxyacetic acid)-containing herbicide was used. Plant samples were grouped into three classes: control (untreated), 1% and 2% herbicide treatment. Retrotransposon activities were investigated by using the inter-retrotransposon amplified polymorphism (IRAP) marker technique. IRAP analyses were performed for Houba (Tos5/Osr13) retrotransposon. Polymorphism ratios were calculated with the Jaccard similarity index, and the significance of polymorphism was evaluated by one-way analysis of variance (ANOVA). We observed that the polymorphism ratios ranged from 8%-90% for Houba among plant samples. ANOVA showed that these variable ratios were statistically significant. Bentazone and the MCPA-containing herbicide increased the retrotransposon activities, and they might be responsible for DNA mutations. This study indicated valuable data for establishing retrotransposon-based short-term mutagenicity test in rice with suitable retrotransposons such as Houba.

show abstract

Section: Resultsmentioning

confidence: 99%

“…In addition to Allium test, the effects of pesticides and herbicides have been commonly analysed by using the MN test and comet assay on plants, fish and also bacteria [47,52,53]. However, these tests are generally used for the identification of herbicide effects.…”

Section: Resultsmentioning

confidence: 99%