Rapid identification and quantitative analysis of chemical constituents of Gentiana veitchiorum by UHPLC-PDA-QTOF-MS

Li, Shan; Wan, Chuan‐Xing; He, Lili; Yan, Zhang; Wang, Kaishun; Yuan, Muhua; Zhang, Zhifeng

doi:10.1016/j.bjp.2016.10.003

Cited by 13 publications

(4 citation statements)

References 12 publications

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“…Moreover, daughter ions at 341 Da (M − H-90-162), and 311 Da (M − H-120-162) due to loss of glucose moiety. These findings indicated the presence of C -glucosyl and O -glucosyl units (Li et al , 33 2017).…”

Section: Resultsmentioning

confidence: 92%

Comparative metabolomics reveals the cytotoxic and anti-inflammatory discriminatory chemical markers of raw and roasted colocynth fruit (Citrullus colocynthis L.)

et al. 2021

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Section: Resultsmentioning

confidence: 92%

Comparative metabolomics reveals the cytotoxic and anti-inflammatory discriminatory chemical markers of raw and roasted colocynth fruit (Citrullus colocynthis L.)

et al. 2021

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“…The major compounds included nine iridoid compounds and their glycosides (1,3,6,7,11,12,17,18,20), nine avonoids and their glycosides (5, 8, 9, 10, 13, 14, 15, 16, 19) and two other compounds and their glycosides (2,4). Among the 20 compounds resolved from the GV extract, compounds 2, 3, 5-9, 11, 12, 14-16 and 19 have previously been found in GV [43][44][45][46][47]. It was of interest that compounds 1, 4, 10, 13, 17, 18 and 20 had not been reported in the literature to be present in GV extracts and were identi ed from this plant for the rst time.…”

Section: Discussionmentioning

confidence: 97%

Exploring the Potential Mechanisms of Action of Gentiana Veitchiorum Hemsl. Extract in the Treatment of Cholestasis using UPLC-MS/MS, Systematic Network Pharmacology, and Molecular Docking

Wang,

Tan,

et al. 2024

CCHTS

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Introduction: Gentiana veitchiorum Hemsl. (GV) has a long history in Tibetan medicine for treating hepatobiliary disease cholestasis. However, the mechanisms mediating its efficacy in treating cholestasis have yet to be determined. background: Gentiana veitchiorum Hemsl. (GV) has a long history in Tibetan medicine to treat cholestasis. However, the mechanisms mediating its efficacy in the treatment of cholestasis have not been determined. Aim: To elucidate the mechanisms of action of GV in the treatment of cholestasis, an integrated approach combining ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis with network pharmacology was established. objective: To elucidate the mechanisms of action of GV in the treatment of cholestasis, an integrated approach combining ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analysis with network pharmacology and molecular docking was established. Materials and Methods: A comprehensive analysis of the chemical composition of GV was achieved by UPLC-MS/MS. Subsequently, a network pharmacology method that integrated target prediction, a protein-protein interaction (PPI) network, gene set enrichment analysis, and a component- target-pathway network was established, and finally, molecular docking and experiments in vitro were conducted to verify the predicted results. Results: Twenty compounds that were extracted from GV were identified by UPLC-MS/MS analysis. Core proteins such as AKT1, TNF, and IL6 were obtained through screening in the Network pharmacology PPI network. The Kyoto Encyclopedia of the Genome (KEGG) pathway predicted that GV could treat cholestasis by acting on signaling pathways such as TNF/IL-17 / PI3K-Akt. Network pharmacology suggested that GV might exert a therapeutic effect on cholestasis by regulating the expression levels of inflammatory mediators, and the results were further confirmed by the subsequent construction of an LPS-induced RAW 264.7 cell model. Conclusions: In this study, UPLC-MS/MS analysis, network pharmacology, and experiment validation were used to explore potential mechanisms of action of GV in the treatment of cholestasis.

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“…Research into the chemical composition of Tibetan medicine Bang Jian began in 1980, focusing mainly on the mainstream species. To date, more than ninety compounds have been isolated and identified by TLC, UV, IR, 1D and 2D NMR, HPLC, GC-MS, HRMS (Li, 2017;, and other methods, mainly including iridoids (1-18), triterpenoids (19-47), flavonoids (47-73), xanthones (74-80), organic acids (81-87) and other compounds (87-92). Among them, the iridoids, flavonoids, triterpenoids, and xanthones have a strong physiological activity and are the main active metabolites in Bang Jian.…”

Section: Phytochemistrymentioning

confidence: 99%

Tibetan medicine Bang Jian: a comprehensive review on botanical characterization, traditional use, phytochemistry, and pharmacology

Li,

Zhang,

Fan

et al. 2023

Front. Pharmacol.

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Tibetan medicine Bang Jian refers to a range of botanical drugs within the Gentiana genus. It serves as a prominent traditional Tibetan botanical drug primarily found in the ethnic minority regions of the Qinghai-Tibet Plateau in China. Traditionally, the dried flowers of Bang Jian, known as “Longdanhua” have been employed in Tibetan medicine to address detoxification, pharyngeal relief, acute and chronic bronchitis, bronchiectasis, lung infections, pulmonary fibrosis, and throat disorders. Surprisingly, there has been no comprehensive review published to date on Tibetan medicine Bang Jian. This passage systematically presents and critically assesses recent advancements in botanical characterization, traditional applications, phytochemistry, pharmacology, and clinical uses of Bang Jian, aiming to provide a scientific foundation for its reasonable use and further exploration. To date, researchers have isolated and identified 92 structurally diverse compounds, with a predominant presence of iridoids, flavonoids, xanthones, and triterpenoids. The crude extracts and metabolites derived from Bang Jian have been found to exhibit a wide range of pharmacological effects, encompassing anti-inflammatory, anti-tumor, anti-bacterial, antiviral, antioxidant, hepatoprotective properties, and protect the respiratory system. Nevertheless, detailed data on the biological effects, metabolic activities, and mechanistic research concerning active monomer metabolites remain insufficient. Consequently, there is a pressing need for comprehensive and in-depth research to guide rational clinical drug usage and evaluate the medicinal attributes of Bang Jian.

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Rapid identification and quantitative analysis of chemical constituents of Gentiana veitchiorum by UHPLC-PDA-QTOF-MS

Cited by 13 publications

References 12 publications

Comparative metabolomics reveals the cytotoxic and anti-inflammatory discriminatory chemical markers of raw and roasted colocynth fruit (Citrullus colocynthis L.)

Comparative metabolomics reveals the cytotoxic and anti-inflammatory discriminatory chemical markers of raw and roasted colocynth fruit (Citrullus colocynthis L.)

Exploring the Potential Mechanisms of Action of Gentiana Veitchiorum Hemsl. Extract in the Treatment of Cholestasis using UPLC-MS/MS, Systematic Network Pharmacology, and Molecular Docking

Tibetan medicine Bang Jian: a comprehensive review on botanical characterization, traditional use, phytochemistry, and pharmacology

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