Potent inhibition of Western Equine Encephalitis virus by a fraction rich in flavonoids and phenolic acids obtained from Achyrocline satureioides

Sabini, María Carola; Cariddi, Laura Noelia; Escobar, Franco Matías; Mañas, Fernando; Comini, Laura R.; Iglesias, Delvis; Larrauri, Mariana; Montoya, Susana C. Núñez; Sereno, José L; Contigiani, Marta Silvia; Cantero, Juan José; Sabini, Liliana

doi:10.1016/j.bjp.2016.05.004

Cited by 5 publications

(3 citation statements)

References 33 publications

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“…En un estudio se evidenció su efecto contra el virus VHS-1 en células Vero. A partir de la purificación de un extracto bruto se obtuvo un IC 50 de 8,5 μg/mL, que se comparó con la quercetina con un IC 50 53 μg/mL (Sabini et al, 2016). Existe una gran variedad de especies de plantas sudamericanas promisorias con actividad antiviral.…”

Section: Plantas De Venezuelaunclassified

Revisión de la actividad antiviral in vitro de plantas sudamericanas como potencial tratamiento contra el virus SARS-CoV-2

Vacas¹,

Vivas²,

Herrera³

et al. 2020

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La alerta por la pandemia del nuevo coronavirus SARS-CoV-2, ha desatado una carrera contra el tiempo en la búsqueda de una posible cura. Algunos de los tratamientos antivirales empleados, son derivados de moléculas obtenidas de plantas. Varios países sudamericanos en donde el uso de terapias naturales, basadas en plantas ancestrales, podrían servir como fuente de estudio contra este virus específicamente. En la presente revisión se analizan varios trabajos de investigación, acerca de la actividad in vitro de plantas con potencial actividad antiviral. El análisis se divide en dos partes, en la primera se realiza un compendio de estudios sobre el uso de plantas euroasiáticas y americanas directamente sobre el virus predecesor SARS-CoV y el coronavirus murino (MHV), donde Paeonia suffruticosa alcanzó los mayores valores tanto de CC50 y EC50. En la segunda parte, se detallan estudios de actividad antiviral in vitro sobre diferentes virus en plantas clasificadas por países de Sudamérica. La familia Asteraceae presentó el mayor porcentaje de uso para el tratamiento de enfermedades respiratorias con un 18 %. Se concluye que la biodiversidad de plantas sudamericanas debe ser aprovechada, por lo que se sugiere realizar un estudio directamente sobre el virus SARS-CoV-2.

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Section: Plantas De Venezuelaunclassified

Revisión de la actividad antiviral in vitro de plantas sudamericanas como potencial tratamiento contra el virus SARS-CoV-2

Vacas¹,

Vivas²,

Herrera³

et al. 2020

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“…have been used as major drug sources due to their low toxicity and increased drug resistance against existing drug molecules [41]. Natural products have been investigated in the past as viral replication inhibitors such as dandelion aqueous extracts against influenza virus [42], bioflavonoids against DENV [43], and JEV [44], chlorogenic acid (CGA) against the replication process in WEEV [45,46] and Enterovirus 71 [47], and piperine (PIP) against hepatitis B virus [48]. Other than these, chemically derived small molecules have also been tested as antiviral such as cyclosporine A and DEBIO-25 against HCV replication [49,50], deubiquitinase derivatives against RNA virus [51,52].…”

Section: Introductionmentioning

confidence: 99%

Alphavirus antivirals targeting RNA‐dependent RNA polymerase domain of nsP4 divulged using surface plasmon resonance

et al. 2022

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Alphaviruses are continuously re-emerging and pose a global threat to human health and currently no antiviral drug is commercially available for alphaviral infections. Alphavirus non-structural protein nsP4, which possesses RNA-dependent RNA polymerase (RdRp) activity, is a potential antiviral target. To date, no antiviral drug is commercially available against alphaviruses. Since RdRp is the key virus-specific enzyme involved in viral genome replication, this study identifies and validates the antiviral efficacy of small molecules targeting alphavirus RdRp. Purified nsP4 was characterized using the surface plasmon resonance (SPR) assay, and the binding affinities of divalent metal ions, ribonucleotides, and in vitro transcribed viral RNA oligonucleotides were obtained in the micromolar (lM) range. Further, four potential inhibitors, piperine (PIP), 2-thiouridine (2TU), pyrazinamide (PZA), and chlorogenic acid (CGA), were identified against nsP4 RdRp using a molecular docking approach. The SPR assay validated the binding of PIP, 2TU, PZA, and CGA to purified nsP4 RdRp with K D of 0.08, 0.13, 0.66, and 9.87 µM, respectively. Initial testing of these molecules as alphavirus replication inhibitors was done using SINV-IRES-Luc virus. Detailed assessment of antiviral efficacy of molecules against CHIKV was performed by plaque reduction assay, qRT-PCR, and immunofluorescence assay. PIP, 2TU, PZA, and CGA showed antiviral potency against CHIKV with EC 50 values of 6.68, 27.88, 36.26, and 53.62 µM, respectively. This study paves the way towards the development of novel broadspectrum alphavirus antivirals targeting nsP4 RdRp.

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“…Retta et al (2012) destaca que a planta medicinal A. satureioides é uma erva aromática anual, tamanho médio e nativa da América, se estende por todo o continente assim como na Europa e África (Salgueiro et al, 2016). Sabini et al (2016) relata que está pertence à família Asteraceae, sendo amplamente consumida por infusão, decocção, maceração e como xarope.…”

Section: Introductionunclassified

Avaliação antimicrobiana e moduladora do extrato de Achyrocline satureioides (Lam.) DC. frente a Escherichia coli

Pinheiro

Neto

Barros

et al. 2021

J. Biotechnol. Biodivers.

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Escherichia coli is a Gram-negative bacterium present in gastrointestinal disorders and is responsible for causing several symptoms, being diarrhea or more common, due to the production of enterotoxins. Note that E. coli strains are becoming resistant due to the use of antimicrobials. Through the search for therapies, medicinal plants have been a source of new research and studies, enabling technical methods in practice. Thus, the objective was to evaluate the antimicrobial and modulatory effect of the ethanol extract of the medicinal plant Achyrocline satureioides against E. coli serotypes. The inflorescences of A. satureioides were collected in March 2019 and, after obtaining the extract, the Minimum Inhibitory Concentration (MIC) assay was performed, and its antibiotic and synergistic activity was evaluated by microdilution. After analyzing the data, it was found that the extract in its form obtained a result of CMI ≥1024μg / mL. A synergistic effect of the extracts was observed when combined with amikacin in the EPEC and ETEC serotypes, as well as with gentamicin in the ETEC serotype. In the other analyzes there was no synergistic or antagonistic effect. The ethanol extract of A. satureioides inflorescences in association with aminoglycosides showed a synergistic effect as tested lines, proving a tendency to the antimicrobial effect, therefore, it is shown that an association of the tested antibiotics increased the antimicrobial potential. Further research will be important for further certification that this is an alternative method in the treatment of gastrointestinal diseases.

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Potent inhibition of Western Equine Encephalitis virus by a fraction rich in flavonoids and phenolic acids obtained from Achyrocline satureioides

Cited by 5 publications

References 33 publications

Revisión de la actividad antiviral in vitro de plantas sudamericanas como potencial tratamiento contra el virus SARS-CoV-2

Revisión de la actividad antiviral in vitro de plantas sudamericanas como potencial tratamiento contra el virus SARS-CoV-2

Alphavirus antivirals targeting RNA‐dependent RNA polymerase domain of nsP4 divulged using surface plasmon resonance

Avaliação antimicrobiana e moduladora do extrato de Achyrocline satureioides (Lam.) DC. frente a Escherichia coli

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