A subchronic toxicity study of ethanol root extract of baked Aconitum flavum in rats

Zhang, Yuanbin; Guan, Enshuang; Zhao, Xin; Wang, Bing; Yin, Lei; Zhang, Liming; Huang, Jun; Fu, Xueyan

doi:10.1016/j.bjp.2016.03.004

Cited by 10 publications

(8 citation statements)

References 12 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Aquatic organisms are likely to be exposed sub-chronically to high atrazine concentrations for periods of up to 3 months in ponds and pools adjacent to fields at the height of the agricultural season as well as chronically to low atrazine concentrations all year round in rivers and lakes downstream (Storrs and Kiesecker, 2004;Wood et al, 2017). Sub-chronic exposures of animals occur over a portion of their life or life cycle (Zhang et al, 2016). Rainfall simulation studies have shown that a high percentage of the atrazine applied on land is lost to ground and surface water sources through surface run-off during the first rain after application, with up to 75% loss occurring over 70 days in loam soils and much less in clay soils where it is prone to transformation into a variety of metabolites (Koskinen and Clay, 1997;Ng and Clegg, 1997;Wallace et al, 2017;Wang et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

Effects of environmentally relevant sub-chronic atrazine concentrations on African clawed frog (Xenopus laevis) survival, growth and male gonad development

Rimayi

Odusanya²,

Weiss

et al. 2018

Aquatic Toxicology

View full text Add to dashboard Cite

Sub-chronic toxicity of environmentally relevant atrazine concentrations on exposed tadpoles and adult male African clawed frogs (Xenopus laevis) was evaluated in a quality controlled laboratory for 90 days. The aim of this study was to determine the effects of atrazine on the survival, growth and gonad development of African clawed frogs. After exposure of tadpoles to atrazine concentrations of 0 (control), 0.01, 200 and 500 μg L in water, mortality rates of 0, 0, 3.3 and 70% respectively were recorded for the 90 day exposure period. Morphometry showed significantly reduced tadpole mass in the 500 μg L atrazine exposed tadpoles (p < 0.05). Light microscopy on testes of adult frogs exposed to the same atrazine concentrations using hematoxylin and eosin (H&E) and Van Gieson staining techniques revealed gonadal atrophy, disruption of germ cell lines, seminiferous tubule structure damage and formation of extensive connective tissue around seminiferous tubules of frogs exposed to 200 μg L and 500 μg L atrazine concentrations. Ultrastructural analysis of the cellular organelles using transmission electron microscopy (TEM) revealed significant amounts of damaged mitochondria in testosterone producing Leydig cells as well as Sertoli cells. Biochemical analysis revealed reduced serum testosterone levels in adult frogs at all exposure levels as well as presence of six atrazine metabolites in frog serum and liver. The results indicate that atrazine concentrations greater than the calculated LC50 of 343.7 μg L cause significant mortality in tadpoles, while concentrations ≥200 μg L adversely affect reproductive health of adult frogs and development of tadpoles sub-chronically exposed to atrazine.

show abstract

Section: Introductionmentioning

confidence: 99%

Effects of environmentally relevant sub-chronic atrazine concentrations on African clawed frog (Xenopus laevis) survival, growth and male gonad development

Rimayi

Odusanya²,

Weiss

et al. 2018

Aquatic Toxicology

View full text Add to dashboard Cite

show abstract

“…e results of the present study revealed that there were no significant effects on erythropoiesis, morphology, or osmotic fragility of RBC [40]. Similarly, the absence of significant changes in white cell counts between polyherbal drug treated rats and healthy control rats reflected the intact condition of the immune system and lack of damages to the tissues [41].…”

Section: Discussionmentioning

confidence: 69%

Acute and Subchronic Toxicity Profile of a Polyherbal Drug Used in Sri Lankan Traditional Medicine

Liyanagamage

Jayasinghe

Attanayake

et al. 2020

Evidence-Based Complementary and Alternative Medicine

View full text Add to dashboard Cite

A polyherbal drug composed of leaves of Murraya koenigii L. Spreng, cloves of Allium sativum L., fruits of Garcinia quaesita Pierre, and seeds of Piper nigrum L. is a popular drug which has been used by indigenous practitioners in Sri Lanka for the treatment of diabetes mellitus and dyslipidemia. The acute toxicity assessment was conducted, following a single oral dose of 0.25–2.0 g/kg in healthy rats, and rats were observed up to 14 days. The hot water extract (1.0 g/kg) and the water : acetone extract (0.5, 1.0, and 1.5 g/kg) were administered to Wistar rats for 28 days in the subchronic study. Hypoglycemic and antihyperglycemic activities (dose response studies) of cold water, hot water, and water : acetone extracts of the polyherbal mixture were evaluated at the doses of 0.5, 1.0, and 1.5 g/kg in healthy and streptozotocin-induced diabetic rats (70 mg/kg, ip), respectively. Acute toxicity study showed that the polyherbal drug did not cause any change in animals throughout the experimental period of 14 days. The administration of the hot water extract and the water : acetone extract of the polyherbal drug for 28 days did not produce changes in the selected biochemical and hematological parameters in Wistar rats (p > 0.05). The histological assessment corroborated the biochemical findings with no significant treatment-related changes in the kidney and liver. The treatment of polyherbal drug significantly lowered the serum glucose concentration compared to the diabetic control rats (p < 0.05) while it did not lead to a severe reduction of glucose concentration in healthy rats. The hot water and water : acetone extracts of the polyherbal drug showed a statistically significant improvement on total area under the glucose tolerance curve in diabetic rats (p < 0.05), reflecting dose-dependent antihyperglycemic effects of the drug. Based on the results, we conclude that the aforementioned antidiabetic polyherbal remedy is free of toxic/adverse effects at the equivalent human therapeutic dose in healthy Wistar rats and would be a safe therapeutic agent for long-term treatments.

show abstract

“…La literatura científica muestra que en los estudios de toxicidad crónica se han evidenciado cambios en la conducta como salivación, horas de sueño, letargo; cambios en la apariencia física, presencia de lesiones que ocasionen dolor o algún otro signo de enfermedad en los animales de experimentación evaluados (20) , estas características clínicas en ratas no se manifestaron durante los 90 días de tratamiento con la asociación del extracto atomizado de rizoma de Curcuma longa, flores de Cordia lutea y hojas de Annona muricata.…”

Section: Discussionunclassified

“…La pérdida de peso en animales de experimentación está asociada a la disminución en la ingesta de alimentos, estos cambios se pueden atribuir a la toxicidad de los productos evaluados (20) ; en el presente estudio se observa incremento del peso corporal de los animales, en caso de las ratas macho a partir de los 30 días, lo que pudo favorecer el incremento de los triglicéridos, así como la reducción del HDL; sin embargo, estudios previos de C. longa determinaron efectos antihiperlipidémicos atribuidos a la curcumina (18) , presumimos que la asociación de ALC reduce ligeramente la actividad de la C. longa, pero dentro de los valores normales (Tabla 2).…”

Section: Discussionunclassified

See 1 more Smart Citation

Toxicidad a 90 días del extracto atomizado de rizoma de Curcuma longa (A4R), Flores de cordia lutea (A4F) y hojas de Annona muricata (A4L) en modelo murino

Arroyo-Acevedo¹,

Franco-Quino²,

Figueroa³

et al. 2017

Rev. per. med. integr.

View full text Add to dashboard Cite

Objetivo: Determinar la seguridad de la asociación del extracto atomizado del rizoma de Curcuma longa (A4R), flores de Cordia lutea (A4F) y hojas de Annona muricata (A4L) a una dosis repetida durante 90 días por vía oral en ratas. Materiales y Métodos: Diseño experimental, se utilizaron 108 ratas Holtzman (54 machos – 54 hembras); se siguió las directrices o normas de la Organisation for Economic Cooperation and Development (OECD) Norma 408; se administró el extracto atomizado durante 90 días por vía oral, se realizaron las observaciones, registro de signos y evolución cada 30 días del peso corporal de los animales, al final se extrajo muestra de sangre para estudio hematológico, hepático, lipídico y antioxidante, se aplicó a prueba ANOVA, considerando el valor p<0.05 para la significancia. Resultados: La asociación de extracto atomizado (ALC) evidenció un incremento significativo del peso para el grupo ALC 200mg/kg; en las ratas macho a partir de los 30 días (p<0.01) y en ratas hembra a partir de los 60 días (p<0.01) manteniendo dicho patrón a los 90 días. Todos los valores hematológicos y bioquímicos durante los 90 días se mantuvieron dentro de parámetros permitidos. Se observó un incremento en la actividad de la Superoxido Dismutasa (SOD) desde los primeros 30 días y manteniendo el mismo hasta el final, independientemente del género (p<0.05). Conclusión: Los hallazgos demuestran que el extracto atomizado del rizoma de Curcuma longa (A4R), flores de Cordia lutea (A4F) y hojas de Annona muricata (A4L) no produce toxicidad al ser administrado por un periodo de 90 días en ratas.

show abstract

A subchronic toxicity study of ethanol root extract of baked Aconitum flavum in rats

Cited by 10 publications

References 12 publications

Effects of environmentally relevant sub-chronic atrazine concentrations on African clawed frog (Xenopus laevis) survival, growth and male gonad development

Effects of environmentally relevant sub-chronic atrazine concentrations on African clawed frog (Xenopus laevis) survival, growth and male gonad development

Acute and Subchronic Toxicity Profile of a Polyherbal Drug Used in Sri Lankan Traditional Medicine

Toxicidad a 90 días del extracto atomizado de rizoma de Curcuma longa (A4R), Flores de cordia lutea (A4F) y hojas de Annona muricata (A4L) en modelo murino

Contact Info

Product

Resources

About