Coumarins of Loricaria ferruginea

Garcia, Gonzalo R. Malca; Hennig, Lothar; Rodríguez, Eric F. Rodríguez; Bussmann, Rainer W.

doi:10.1016/j.bjp.2016.02.006

Cited by 4 publications

(1 citation statement)

References 11 publications

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“…Our methodology, which is founded on liquid-liquid chromatography, achieved exceptional results that included no irreversible adsorption, fast elution, higher recovery, and superior separation efficiency. Compared with the reported methods for the separation of compound I from natural products (the hexane extract of Loricaria ferruginea) using solid-liquid chromatography [27], our process yielded 103-fold higher production, required about one-third of the separation time, and consumed about one-fifth of the solvent, demonstrating its economic and environmentally-friendly nature. Likewise, liquid-liquid chromatography yields were about 5-fold higher for compound II [28], 300-fold higher for compound III [18], 320-fold higher for compound IV [29], and 500-fold higher for compound V [30].…”

Section: Liquid-liquid Chromatographic Separationmentioning

confidence: 91%

Coumarins from Jinhua Finger Citron: Separation by Liquid–Liquid Chromatography and Potential Antitumor Activity

Wang,

Huang,

Zhou

et al. 2023

Molecules

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In this paper, liquid–liquid chromatography was introduced for the first time for the separation of fingered citron (Citrus medica L. var. sarcodactylis Swingle). The fingered citron cultivated in Jinhua is of significant industrial and medicinal value, with several major coumarin compounds detected in its extract. Therefore, further separation for higher purity was of necessity. A preparative liquid–liquid chromatographic method was developed by combining two elution modes (isocratic and step-gradient) with selection according to different polarities of the target sample. Five coumarin derivatives—5,7-dimethoxycoumarin (52.6 mg, 99.6%), phellopterin (4.9 mg, 97.1%), 5-prenyloxy-7-methoxycoumarin (6.7 mg, 98.7%), 6-hydroxy-7-methoxycoumarin (7.1 mg, 82.2%), and byakangelicol (10.5 mg, 90.1%)—with similar structures and properties were isolated on a large scale from 100 mg of petroleum ether (PE) extract and 100 mg of ethyl acetate (EA) extract in Jinhua fingered citron. The productivity was much improved. The anti-growth activity of the isolated coumarins was evaluated against three cancer cell lines (HeLa, A549, and MCF7) with an MTT assay. The coumarins demonstrated potential anti-tumor activity on the HeLa cell line, with 5,7-dimethoxycoumarin in particular exhibiting the best anti-growth activity (IC50 = 10.57 ± 0.24 μM) by inhibiting proliferation. It inhibited colony formation and reduced the size of the tumor sphere in a concentration-dependent manner. The main mechanism was confirmed as inducing apoptosis. This work was informative for further studies aimed at exploring new natural-product-based antitumor agents.

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Section: Liquid-liquid Chromatographic Separationmentioning

confidence: 91%

Coumarins from Jinhua Finger Citron: Separation by Liquid–Liquid Chromatography and Potential Antitumor Activity

Wang,

Huang,

Zhou

et al. 2023

Molecules

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Coumarins: Analytical and Preparative Techniques

Luca

Głowniak

Skalicka‐Woźniak

2018

Encyclopedia of Analytical Chemistry

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Coumarins are nowadays an important group of organic compounds from natural and synthetic sources, possessing a wide range of pharmacological activities. Coumarins comprise a large group of phenolic substances, which occur in plants as secondary metabolites. On account of the significance of this group of compounds, the development of effective methods for their extraction, separation, qualitative, and quantitative analysis, as well as for purification remains of high importance. The purpose of the article is to present information about methods of analysis and separation of this important group of naturally occurring compounds.

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Coumarins and lignans from the roots of Zanthoxylum nitidum

Wang

Zhu

et al. 2022

Biochemical Systematics and Ecology

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Coumarins of Loricaria ferruginea

Cited by 4 publications

References 11 publications

Coumarins from Jinhua Finger Citron: Separation by Liquid–Liquid Chromatography and Potential Antitumor Activity

Coumarins from Jinhua Finger Citron: Separation by Liquid–Liquid Chromatography and Potential Antitumor Activity

Coumarins: Analytical and Preparative Techniques

Coumarins and lignans from the roots of Zanthoxylum nitidum

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