In vitro and in vivo evaluation of efficacy and safety of photoprotective formulations containing antioxidant extracts

Reis-Mansur, Maria Cristina Pinheiro Pereira; Leitão, Suzana Guimarães; Cerqueira-Coutinho, Cristal; Vermelho, Alane Beatriz; Silva, Ronald S.; Presgrave, Octávio Augusto França; Leitão, Álvaro A.C.; Leitão, Gilda Guimarães; Ricci-Júnior, Eduardo; Santos, Elisabete Pereira dos

doi:10.1016/j.bjp.2015.11.006

Cited by 65 publications

(67 citation statements)

References 39 publications

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“…The Mansur method (Mansur, et al, 1986) was employed to determine the in vitro SPF of the nonpigmented base without polymers (NPB1) because it is effective and fast. In addition, this method also shows a good correlation with results that can be found at the in vivo assays, and it is widely described at literature (Mansur et al, 2016).…”

Section: Determination Of the In Vitro Spf Of Non-pigmented Npb1 Basesupporting

confidence: 52%

“…These solutions were then subjected to analysis in a JASGO V-630 UV/VIS spectrophotometer to determine absorbance in the wavelength range 290-320 nm. Absorbance was read at each 5 nm interval as shown in Table IV (Mansur, et al, 1986;Santos et al, 1999;Freitas et al, 2001;Mansur et al, 2016).…”

Section: Determination Of the In Vitro Spf Of Non-pigmented Npb1 Basementioning

confidence: 99%

“…Physicochemical characteristics, such as phase separation (centrifugation test), density, and pH were also assessed (Anvisa, 2004;Smaoui et al, 2013). The SPF variation of the formulations was also observed during the stability tests (Miksa, Lutz, Guy, 2015;Cerqueira-Coutinho et al, 2015;Mansur et al, 2016).…”

Section: Stability Study and Physicochemical Testsmentioning

confidence: 99%

“…SPF variations were evaluated in triplicate by transmittance spectrophotometry with a Labsphere ® (Miksa, Lutz, Guy, 2015;Cerqueira-Coutinho et al, 2015;Mansur et al, 2016).…”

Section: Spf Variationmentioning

confidence: 99%

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Multifunctional photoprotection: corrective makeup for cutaneous dyschromias

Gonçalves

Giovanini

Cerqueira

et al. 2018

Braz. J. Pharm. Sci.

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The aim of this study was to develop and evaluate a corrective and photoprotective makeup for patients with dyschromias. An emulsion was prepared and pigment mixtures were incorporated in the formulation, producing five shades of corrective makeup: BEIGE (I, II, III), BRONZE and TAN. The sun protection factor (SPF) and UVA/UVB ratio of the corrective makeup were determined using spectrophotometry with a Labsphere ® analyser. The spreadability, occlusivity, stability, and photostability of the photoprotective formulations were also evaluated. For all formulations there was no statistical difference among them (p > 0.05) in terms of spreadability, occlusivity and SPF. They were considered to be photostable under solar radiation, with variations in SPF value and UVA/UVB ratio lower than 20%. The corrective makeup presented average-to-high UVB photoprotection and broad spectrum photoprotection. After 90 days, pH, density and SPF values showed no significant differences among formulations (p>0.05). All corrective makeup presented separation of the pigments, however, they returned to a homogeneous aspect and to the original color shade after shaking. The corrective makeup presented a fine texture, little brightness, and a homogeneous, dry-to-the-touch aspect. This work may benefit patients with dyschromias, improving their quality of life, besides promoting photoprotection and covering the skin blemishes.

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Section: Determination Of the In Vitro Spf Of Non-pigmented Npb1 Basesupporting

confidence: 52%

Section: Determination Of the In Vitro Spf Of Non-pigmented Npb1 Basementioning

confidence: 99%

Section: Stability Study and Physicochemical Testsmentioning

confidence: 99%

“…SPF variations were evaluated in triplicate by transmittance spectrophotometry with a Labsphere ® (Miksa, Lutz, Guy, 2015;Cerqueira-Coutinho et al, 2015;Mansur et al, 2016).…”

Section: Spf Variationmentioning

confidence: 99%

See 2 more Smart Citations

Multifunctional photoprotection: corrective makeup for cutaneous dyschromias

Gonçalves

Giovanini

Cerqueira

et al. 2018

Braz. J. Pharm. Sci.

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“…massambabensis Vaz na concentração de 1% (m/m).A emulsão desenvolvida incluiu três filtros solares -benzofenona 3, metoxicinamato de octila e octocrileno, cada um em 5%, dando um valor total de 15%. Semelhante ao nosso estudo, a adição do extrato aumentou o FPS dos filtros (de 13 até 18), mas o produto sozinho não teve efeito fotoprotetor(MANSUR et al, 2016). Já para uma outra espécie Garcinia brasiliensis…”

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Atividade antioxidante e fotoprotetora de bambus nativos do Sudeste brasileiro

Wróblewska¹

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Application of Kenaf Seed Oil‐Nanostructured Lipid Carrier to Palm‐Based α‐Tocopherol Cream for Photoprotection

Chu

Hasan

Tan

et al. 2021

J Americ Oil Chem Soc

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The application of a nanostructured lipid carrier (NLC) to ultraviolet (UV) filter encapsulation was found to enhance its safe use. In this work, a kenaf seed oil‐NLC (KSO‐NLC) coloaded with 1% diethylamino hydroxybenzoyl hexyl benzoate and 1% ethylhexyl triazone was used as an active ingredient in α‐tocopherol cream to develop a photoprotective prototype. It was then subjected to further analysis to determine its physical properties, storage stability, and cytotoxicity. The ratio of KSO‐NLC to α‐tocopherol cream was optimized based on the sun protection factor (SPF) value using a UV transmittance analyzer. The physical properties of the samples were analyzed, and the amount of α‐tocopherol was quantified by ultra‐high‐performance liquid chromatography. The optimized sample was then evaluated for in vitro antioxidant activities using 2,2‐diphenyl‐1‐picrylhydrazyl (DPPH) and 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid assays. Meanwhile, in vitro cytotoxicity was studied on a normal human dermal fibroblast cell line using thiazolyl blue tetrazolium bromide and cell counting kit‐8 assays. The sample of KSO‐NLC to α‐tocopherol cream in a ratio of 1:2 (N3) showed SPF value >50. Besides, the samples showed microbial stability and sustainable α‐tocopherol content upon 12 weeks of storage. The cytotoxicity evaluation of N3 and α‐tocopherol cream (N5) showed >100% cell proliferation, which indicated that there is no side effect on the cell growth, while triggering the cell proliferation with the presence of bioactive compounds. Overall, the findings of the study are promising for the development of new sunscreen formulation with the utilization of KSO‐NLC and palm‐based α‐tocopherol cream.

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In vitro and in vivo evaluation of efficacy and safety of photoprotective formulations containing antioxidant extracts

Cited by 65 publications

References 39 publications

Multifunctional photoprotection: corrective makeup for cutaneous dyschromias

Multifunctional photoprotection: corrective makeup for cutaneous dyschromias

Atividade antioxidante e fotoprotetora de bambus nativos do Sudeste brasileiro

Application of Kenaf Seed Oil‐Nanostructured Lipid Carrier to Palm‐Based α‐Tocopherol Cream for Photoprotection

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