Evaluation of acetylcholinesterase inhibitory activity of Brazilian red macroalgae organic extracts

Machado, Levi Pompermayer; Carvalho, Luciana Retz de; Young, Maria Cláudia M.; Cardoso-Lopes, Elaine Monteiro; Centeno, Danilo da Cruz; Zambotti-Villela, Leonardo; Colepicolo, Pio; Yokoya, Nair S.

doi:10.1016/j.bjp.2015.09.003

Cited by 40 publications

(14 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Thus, there is an urge to find safer AChE inhibitors from natural sources. 49,51 In this study, the methanolic extract obtained using homogenization (HAE-methanol) was the most potent inhibitor of AChE and BChE (Table 3).…”

Section: Enzyme Inhibitory Propertiesmentioning

confidence: 79%

“…However, these drugs have showed limited efficacy and unfavorable effects such as gastrointestinal disturbances and hepatotoxicity. Thus, there is an urge to find safer AChE inhibitors from natural sources . In this study, the methanolic extract obtained using homogenization (HAE–methanol) was the most potent inhibitor of AChE and BChE (Table ).…”

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis

et al. 2019

View full text Add to dashboard Cite

BACKGROUND In this study, we aimed to evaluate the influence of different extraction procedures [decoction, homogenizer‐assisted extraction (HAE), infusion, maceration, Soxhlet and ultrasound‐assisted extraction (UAE)] on the chemical profiling and biological properties of methanol and water extracts of Pulicaria dysenterica (L.) Bernh. The chemical profiles of the extracts were evaluated by high‐performance liquid chromatography coupled to electrospray ionization and time‐of‐flight mass spectrometry (HPLC‐ESI‐TOF‐MS). The antioxidant properties and enzymes (lipase, α‐amylase, α‐glucosidase, tyrosinase and cholinesterases) inhibitory potential of the extracts were evaluated. RESULTS The chemical profiles were dependent on the type of extraction methods as well as on the type of solvent. The methanolic extracts showed higher levels of total phenolic, flavonoid, and phenolic acid content, while the highest total flavonol content was observed in the HAE–water extract. Forty different compounds were identified from P. dysenterica. In relation to the potential in vitro anti‐diabetic effects, the highest activity against the studied key enzymes was observed for the macerated extract (α‐amylase: 0.58 ± 0.03 and α‐ glucosidase: 1.65 ± 0.03 mmol ACAE g−1). The HAE–methanol extract was the most potent inhibitor of cholisterases, whereas the highest activities against tyrosinase were observed for UAE–methanol extract, followed by macerated and Soxhlet. The inhibitory activity of the studied extracts against lipase were in the order: soxhlet > macerated> HAE–methanol > UAE–methanol. CONCLUSION This study has established scientific baseline data on the therapeutic properties of P. dysentrica, thereby advocating the need for further investigations in an endeavour to develop novel pharmaceuticals from this plant. © 2019 Society of Chemical Industry

show abstract

Section: Enzyme Inhibitory Propertiesmentioning

confidence: 79%

Section: Resultsmentioning

confidence: 99%

Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis

et al. 2019

View full text Add to dashboard Cite

show abstract

“…AChE is localized in synaptic gaps of the central and peripheral nervous system and is responsible for the breakdown of acetylcholine (ACh). Thus, AChE terminates nerve impulses through the loss of basal forebrain cholinergic neurons and by reducing the level of the neurotransmitter ACh, which is characteristic of AD as a chronic neurodegenerative disorder [ 29 , 30 ]. Inhibition of AChE increases cholinergic functions, and hence, it is the therapeutic target for not only managing AD, but also for moderating other disorders such as myasthenia gravis, glaucoma, and Lewy body dementia [ 30 ].…”

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory, Anti-Diabetic, and Anti-Alzheimer’s Effects of Prenylated Flavonoids from Okinawa Propolis: An Investigation by Experimental and Computational Studies

et al. 2018

View full text Add to dashboard Cite

Okinawa propolis (OP) and its major ingredients were reported to have anti-cancer effects and lifespan-extending effects on Caenorhabditis elegans through inactivation of the oncogenic kinase, p21-activated kinase 1 (PAK1). Herein, five prenylated flavonoids from OP, nymphaeol-A (NA), nymphaeol-B (NB), nymphaeol-C (NC), isonymphaeol-B (INB), and 3′-geranyl-naringenin (GN), were evaluated for their anti-inflammatory, anti-diabetic, and anti-Alzheimer’s effects using in vitro techniques. They showed significant anti-inflammatory effects through inhibition of albumin denaturation (half maximal inhibitory concentration (IC50) values of 0.26–1.02 µM), nitrite accumulation (IC50 values of 2.4–7.0 µM), and cyclooxygenase-2 (COX-2) activity (IC50 values of 11.74–24.03 µM). They also strongly suppressed in vitro α-glucosidase enzyme activity with IC50 values of 3.77–5.66 µM. However, only INB and NA inhibited acetylcholinesterase significantly compared to the standard drug donepezil, with IC50 values of 7.23 and 7.77 µM, respectively. Molecular docking results indicated that OP compounds have good binding affinity to the α-glucosidase and acetylcholinesterase proteins, making non-bonded interactions with their active residues and surrounding allosteric residues. In addition, none of the compounds violated Lipinski’s rule of five and showed notable toxicity parameters. Density functional theory (DFT)-based global reactivity descriptors demonstrated their high reactive nature along with the kinetic stability. In conclusion, this combined study suggests that OP components might be beneficial in the treatment of inflammation, type 2 diabetes mellitus, and Alzheimer’s disease.

show abstract

“…DCM/MeOH extracts (100 μg) of (1) Hypnea musciformis , (2) Pterocladia capillacea , and (3) Ochtodes secundiramea . TLC elution system: hexane:ethyl acetate:methanol (2:7:1 v / v / v ) [ 89 ].…”

Section: Figurementioning

confidence: 99%

Therapeutic Uses of Red Macroalgae

2020

View full text Add to dashboard Cite

Red Seaweed “Rhodophyta” are an important group of macroalgae that include approximately 7000 species. They are a rich source of structurally diverse bioactive constituents, including protein, sulfated polysaccharides, pigments, polyunsaturated fatty acids, vitamins, minerals, and phenolic compounds with nutritional, medical, and industrial importance. Polysaccharides are the main components in the cell wall of red algae and represent about 40–50% of the dry weight, which are extensively utilized in industry and pharmaceutical compounds, due to their thickening and gelling properties. The hydrocolloids galactans carrageenans and agars are the main red seaweed cell wall polysaccharides, which had broad-spectrum therapeutic characters. Generally, the chemical contents of seaweed are different according to the algal species, growth stage, environment, and external conditions, e.g., the temperature of the water, light intensity, nutrient concentrations in the ecosystem. Economically, they can be recommended as a substitute source for natural ingredients that contribute to a broad range of bioactivities like cancer therapy, anti-inflammatory agents, and acetylcholinesterase inhibitory. This review touches on the main points of the pharmaceutical applications of red seaweed, as well as the exploitation of their specific compounds and secondary metabolites with vital roles.

show abstract

Evaluation of acetylcholinesterase inhibitory activity of Brazilian red macroalgae organic extracts

Cited by 40 publications

References 42 publications

Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis

Innovative perspectives on Pulicaria dysenterica extracts: phyto‐pharmaceutical properties, chemical characterization and multivariate analysis

Anti-Inflammatory, Anti-Diabetic, and Anti-Alzheimer’s Effects of Prenylated Flavonoids from Okinawa Propolis: An Investigation by Experimental and Computational Studies

Therapeutic Uses of Red Macroalgae

Contact Info

Product

Resources

About