Ragged Robin (Lychnis flos-cuculi) - a plant with potential medicinal value

Maliński, Michał P.; Michalska, Anna; Tomczykowa, Monika; Thiem, Barbara

doi:10.1016/j.bjp.2014.11.004

Cited by 8 publications

(10 citation statements)

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“…Though the species is not rare, its population is on decline. Moreover, the fragmentation of populations of L. flos-cuculi leads to the loss of genetic diversity and inbreeding (reviewed by Maliński et al 2014). The above factors encouraged the authors to introduce this species into in vitro cultures and to multiply its biomass under controlled conditions for the phytochemical analyses.…”

Section: Discussionmentioning

confidence: 99%

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Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Maliński

Kikowska

Kruszka

et al. 2019

Plant Cell Tiss Organ Cult

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Lychnis flos-cuculi L. is a species containing ecdysteroids, triterpenoid saponins, flavonoids, and phenolic acids, and therefore is a plant of potential medicinal value. In the presented research, diverse in vitro cultures of this taxon were developed to obtain the uniform material capable of producing ecdysteroids, including micropropagated plantlets, shoot cultures, liquid agitated whole plant cultures with fast-growing roots, and callus. A protocol of micropropagation through axillary bud formation was established using plant growth regulators at different concentrations and combinations. All the variants of plant growth regulator supplementation significantly affected a shoot proliferation rate ranging from 8 to 16 plantlets per explant, depending on the medium; DNA content of all the studied plant materials was similar. The thin-layer chromatography analysis of the extracts revealed the presence of ecdysteroids in every plant material apart from callus. The content of 20-hydroxyecdysone and polypodine B was evaluated by high-performance liquid chromatography. Agitated plantlets were in vitro cultures that efficiently formed abundant root biomass with significant concentrations of ecdysteroids. In vitro-derived adventitious roots contained twofold higher content of ecdysteroids than those of intact plants. The organs of flowering in vitro-propagated plants, transferred to experimental plot, contained twice as much ecdysteroids when compared to the organs of plants from the natural site, among which flowers were the richest in ecdysteroids. The results revealed that adventitious roots from L. flos-cuculi agitated cultures can be considered as an alternative biotechnological source of biomass rich in pharmaceutically active ecdysteroids. Key message Diverse types of Lychnis flos-cuculi in vitro cultures have been developed. HPLC analysis demonstrated much higher ecdysteroid content in cultures and regenerated plants when compared to natural site plants. Keywords Micropropagation • Callus • 20-Hydroxyecdysone • Polypodine B • Agitated plant cultures • Genome size Abbreviations 2,4-D 2,4-Dichlorophenoxyacetic acid 20HE 20-Hydroxyecdysone BAP N 6-benzylaminopurine Dic Dicamba (3,6-dichloro-2-methoxybenzoic acid) IAA Indole-3-acetic acid Kin Kinetin (N 6-furfuryladenine) MS Murashige & Skoog medium NAA 1-Naphthaleneacetic acid PGRs Plant growth regulators Pic Picloram (4-amino-3,5,6-trichloro-2-pyridinecarboxylic acid) polB Polypodine B (5β,20-dihydroxyecdysone) TDZ Thidiazuron (1-phenyl-3-(1,2,3-thiadiazol-5-yl)-urea TLC Thin-layer chromatography Communicated by Ali R. Alan.

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Section: Discussionmentioning

confidence: 99%

“…So far, only two oleanane-type triterpenoid saponins have been isolated from L. flos-cuculi, namely coronosides A and B (Bucharow et al 1974), while the rest of saponin complex remains unidentified. The secondary metabolites present in L. flos-cuculi were described in a review by Maliński et al (2014).…”

Section: Introductionmentioning

confidence: 99%

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Maliński

Kikowska

Kruszka

et al. 2019

Plant Cell Tiss Organ Cult

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“…The in vitro regenerated shoots were successfully rooted and transferred into the soil. Preliminary chromatographic analysis by TLC and HPLC indicated that multiple shoots and roots from in vitro-derived plants maintained the ability to accumulate phytoecdysteroids, identified as 20-hydroxyecdysone and polypodine B (Thiem et al 2013 ; Maliński et al 2014 ; Napierała unpublished).…”

Section: Ecdysteroid Production In Plant In Vitro Culturesmentioning

confidence: 99%

Ecdysteroids: production in plant in vitro cultures

et al. 2016

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Ecdysteroids are secondary metabolites, widely distributed in the animal and plant kingdoms. They have a wide range of pharmacological effects in vertebrates, including mammals, most of which are beneficial for humans. Therefore, they have become compounds of interest for the pharmaceutical industry due to their adaptogenic, anabolic, hypoglycaemic, hypocholesterolaemic and antimicrobial activities, which are still being researched. Nowadays, ecdysteroids are present as active ingredients in bodybuilding supplements. Because of their complex structures, their chemical synthesis seems unprofitable and impractical. Due to high content of ecdysteroids in many plants, they are primarily obtained by extraction of the plant material. Plant in vitro cultures provide an alternative source of these compounds, helping to avoid problems associated with field production—such as variable yield or dependence on environmental factors, as well as limited availability of natural resources. Plant cell and tissue cultures may be suggested as alternatives for the production of plant biomass rich in pharmaceutically active ecdysteroids. Moreover, the use of common biotechnological strategies, such as elicitation or precursor feeding, may further increase the yield and improve production of these compounds. In this paper, we describe general information about ecdysteroids: their structure, biosynthesis, distribution, role in plants, and we review recent studies on micropropagation of ecdysteroid-producing plants and cell cultures, and potential ability of ecdysteroids enhancement in in vitro cultures.

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“…Until recently, little was known about the phytochemical constituents of this plant. However, it is now acknowledged that it contains phytoecdysteroids, triterpenoid saponins, flavonoids, and phenolic acids [ 1 , 2 , 3 , 4 , 5 , 6 ]. The species was introduced to in vitro cultures by our research team and the protocols of micropropagation and callus induction and development were established for the first time.…”

Section: Introductionmentioning

confidence: 99%

Two Ecdysteroids Isolated from Micropropagated Lychnis flos-cuculi and the Biological Activity of Plant Material

et al. 2021

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Genetically uniform plant material, derived from Lychnis flos-cuculi propagated in vitro, was used for the isolation of 20-hydroxyecdysone and polypodine B and subjected to an evaluation of the antifungal and antiamoebic activity. The activity of 80% aqueous methanolic extracts, their fractions, and isolated ecdysteroids were studied against pathogenic Acanthamoeba castellani. Additionally, a Microtox® acute toxicity assay was performed. It was found that an 80% methanolic fraction of root extract exerts the most potent amoebicidal activity at IC50 of 0.06 mg/mL at the 3rd day of treatment. Both ecdysteroids show comparable activity at IC50 of 0.07 mg/mL. The acute toxicity of 80% fractions at similar concentrations is significantly higher than that of 40% fractions. Crude extracts exhibited moderate antifungal activity, with a minimum inhibitory concentration (MIC) within the range of 1.25–2.5 mg/mL. To the best of our knowledge, the present report is the first to show the biological activity of L. flos-cuculi in terms of the antifungal and antiamoebic activities and acute toxicity. It is also the first isolation of the main ecdysteroids from L. flos-cuculi micropropagated, ecdysteroid-rich plant material.

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Ragged Robin (Lychnis flos-cuculi) - a plant with potential medicinal value

Cited by 8 publications

References 57 publications

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Various in vitro systems of Ragged Robin (Lychnis flos-cuculi L.): a new potential source of phytoecdysteroids?

Ecdysteroids: production in plant in vitro cultures

Two Ecdysteroids Isolated from Micropropagated Lychnis flos-cuculi and the Biological Activity of Plant Material

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