Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

Bortoli, Stella; Oliveira‐Silva, Diogo; Krüger, Thomas; Dörr, Felipe Augusto; Colepicolo, Pio; Volmer, Dietrich A.; Pinto, Ernani

doi:10.1016/j.bjp.2014.07.019

Cited by 30 publications

(15 citation statements)

References 47 publications

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“…The abundance of toxins during blooms depends on the presence of toxic strains within the cyanobacterial population [ 22 ]. Different species have been shown to produce specific toxins or even variants [ 23 , 24 , 25 ]. However, in toxic strains, the environmental factors controlling expression of the toxin synthetase genes are still a contentious issue [ 26 ].…”

Section: Introductionmentioning

confidence: 99%

Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins

Mantzouki

Lürling

Fastner

et al. 2018

Toxins

159

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Insight into how environmental change determines the production and distribution of cyanobacterial toxins is necessary for risk assessment. Management guidelines currently focus on hepatotoxins (microcystins). Increasing attention is given to other classes, such as neurotoxins (e.g., anatoxin-a) and cytotoxins (e.g., cylindrospermopsin) due to their potency. Most studies examine the relationship between individual toxin variants and environmental factors, such as nutrients, temperature and light. In summer 2015, we collected samples across Europe to investigate the effect of nutrient and temperature gradients on the variability of toxin production at a continental scale. Direct and indirect effects of temperature were the main drivers of the spatial distribution in the toxins produced by the cyanobacterial community, the toxin concentrations and toxin quota. Generalized linear models showed that a Toxin Diversity Index (TDI) increased with latitude, while it decreased with water stability. Increases in TDI were explained through a significant increase in toxin variants such as MC-YR, anatoxin and cylindrospermopsin, accompanied by a decreasing presence of MC-LR. While global warming continues, the direct and indirect effects of increased lake temperatures will drive changes in the distribution of cyanobacterial toxins in Europe, potentially promoting selection of a few highly toxic species or strains.

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Section: Introductionmentioning

confidence: 99%

Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins

Mantzouki

Lürling

Fastner

et al. 2018

Toxins

159

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“…The hepatotoxic microcystins, synthesized by an integrated peptide-polyketide synthetase [ 5 , 6 ], are cyclic peptides and share a common structure {cyclo(-Adda-D-Glu-Mdha-D-Ala-L-X-D-MeAsp-L-Z)}, where X and Z represent variable amino acids [ 7 ]. Microcystin-LR (MC-LR) and microcystin-RR (MC-RR) are the most common variants among over 90 microcystin analogues found [ 8 , 9 ]. The former is five times more toxic, as indicated by LD 50 in mice [ 10 ].…”

Section: Introductionmentioning

confidence: 99%

Multi-Year Assessment of Toxic Genotypes and Microcystin Concentration in Northern Lake Taihu, China

Shan

Lin

et al. 2016

Toxins

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Lake Taihu is the third-largest freshwater lake in China and has been suffering from cyanobacterial blooms for over two decades. The northern part of the lake, Meiliang Bay, is known to be at high risk of dense and sustained Microcystis blooms and toxins. This study aimed to investigate and record the annual and seasonal dynamics of toxic genotype, Microcystis morphospecies succession and microcystin variation. It also aimed to find out the underlying driving factors influencing the dynamic changes. Microcystin (MC) and the Microcystis genotype were quantified using HPLC and quantitative real-time PCR, respectively. Our study, over three consecutive years, showed that the pattern of morphospecies succession was seasonally distinct and annually consistent. During the same period in 2012, 2013 and 2014, the average MC were, on dry weight basis, 733 μg·g−1, 844 μg·g−1, 870 μg·g−1, respectively. The proportion of toxic Microcystis accounted for 41%, 44% and 52%, respectively. Cell bound microcystin was found to correlate with the percentage of toxic Microcystis. Based on historical and current data, we conclude that annual bloom toxicity was relatively stable or possibly increased over the last decade.

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“…We found that the lower concentrations of MC (191.87 μg L −1 ) were more toxic than higher concentrations (1194.84 μg L −1 ). The present work did not consider other metabolites produced by cyanobacteria, such as protease inhibitors, and structural variations of the MCs, such as MC-RR, MC-LR, MC-YR, MC-LF, MC-LW (Dörr et al 2010;Bortoli et al 2014;Rastogi et al 2014), STXs, andGTXs (Ferrão-Filho et al 2010;Wiese et al 2010;Carneiro et al 2013). Kuster and Elert (2013) verified a negative effect in the development of D. pulex after exposure to different concentrations of the two lineages of non-MC producing M. aeruginosa, which contained the inhibitor chymotrypsin or trypsin.…”

Section: Resultsmentioning

confidence: 99%

Evaluation of the Ecotoxicological Effects of Microcystis aeruginosa and Cylindrospermopsis raciborskii on Ceriodaphnia dubia Before and After Treatment with Ultrasound

Lira

Moreira

Tonello

et al. 2017

Water Air Soil Pollut

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Bodies of water contaminated by cyanobacteria and their neuro-and hepatotoxins have caused environmental and public health issues all over the world. Therefore, determining safe concentrations in water for multiple uses to protect aquatic biota and identify forms of remediation are of broad interest. In this study, we isolated strains of the cyanobacteria Microcystis aeruginosa and Cylindrospermopsis raciborskii, which produce microcystin (MC) and saxitoxin (STX), respectively. Ecotoxicological tests using suspensions of lysed lyophilizated cells with concentrations of toxins equivalent to those permitted by legislation for potability (1 μg L −1 for MC and 3 μg L −1 for STX) did not result in significant mortality of the model organism, Ceriodaphnia dubia, where as concentrations five times greater resulted in decreased survival for both toxins. However, reproduction was significantly reduced even in the lower concentrations, indicating that the currently permitted standards are not safe for environmental protection. When cyanotoxins were treated with ultrasound, mortalities were no longer significant, independent of concentrations. Although reproduction was still lower in relation to the control, it was significantly higher when compared to the results obtained before ultrasound. Ultrasound has been previously applied to cyanobacteria cell lysis, but this is the first study to investigate the ecotoxicological effects of ultrasound on cyanotoxins. Using new test organisms and different times and potency of sonication will permit the development of more efficient techniques for the remediation of these toxins and the development of more adequate parameters for the protection of aquatic life.

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Growth and microcystin production of a Brazilian Microcystis aeruginosa strain (LTPNA 02) under different nutrient conditions

Cited by 30 publications

References 47 publications

Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins

Temperature Effects Explain Continental Scale Distribution of Cyanobacterial Toxins

Multi-Year Assessment of Toxic Genotypes and Microcystin Concentration in Northern Lake Taihu, China

Evaluation of the Ecotoxicological Effects of Microcystis aeruginosa and Cylindrospermopsis raciborskii on Ceriodaphnia dubia Before and After Treatment with Ultrasound

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