Chemical composition and efficacy in the egg-hatching inhibition of essential oil of Piper aduncum against Haemonchus contortus from sheep

Oliveira, Gisele; Vieira, Thallyta Maria; Nunes, Vanessa Francisca; Ruas, Munic de Oliveria; Duarte, Eduardo Robson; Moreira, Davyson de Lima; Kaplan, Maria Auxiliadora Coelho; Martins, Ernane Ronie

doi:10.1016/j.bjp.2014.07.004

Cited by 26 publications

(14 citation statements)

References 16 publications

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“…In the present study, EO anti-hatching activity was only evident against susceptible eggs at the highest concentration (55.8% inhibition with 12.5 mg/mL), which was statistically different of the other concentrations on McMaster isolate, as well as at the same concentration (12.5 mg/mL) on the Embrapa2010 isolate. Other work using the EHT showed that the EO from P. aduncum L. was 95% efficacious at 12 mg/mL against H. contortus eggs (OLIVEIRA et al, 2014). We believe this divergent results stem from the chemical composition of EOs, which in the previous study primarily contained 1,8-cineole (55.8% against 0% dillapiole), whereas in the present study 76.2% of the oil was composed of dillapiole with no 1,8-cineole.…”

Section: Discussioncontrasting

confidence: 55%

Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds

Gaínza

Fantatto

Chaves

et al. 2016

Rev. Bras. Parasitol. Vet.

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The anthelminthic activity of the essential oil (EO) of Piper aduncum L. was tested in vitro on eggs and larvae of resistant (Embrapa2010) and susceptible (McMaster) isolates of Haemonchus contortus. The EO was obtained by steam distillation and its components identified by chromatography. EO concentrations of 12.5 to 0.02 mg/mL were used in the egg hatch test (EHT) and concentrations of 3.12 to 0.01 mg/mL in the larval development test (LDT). Inhibition concentrations (IC) were determined by the SAS Probit procedure, and significant differences assessed by ANOVA followed by Tukey's test. In the EHT, the IC 50 for the susceptible isolate was 5.72 mg/mL. In the LDT, the IC 50 and IC 90 were, respectively, 0.10 mg/mL and 0.34 mg/mL for the susceptible isolate, and 0.22 mg/mL and 0.51 mg/mL for the resistant isolate. The EO (dillapiole 76.2%) was highly efficacious on phase L 1 . Due to the higher ICs obtained for the resistant isolate, it was raised the hypothesis that dillapiole may have a mechanism of action that resembles those of other anthelmintic compounds. We further review and discuss studies, especially those conducted in Brazil, that quantified the major constituents of P. aduncum-derived EO.Keywords: Isolates, Haemonchus contortus, cross resistance, dillapiole, synergy. ResumoEste estudo avaliou a atividade anti-helmíntica in vitro do óleo essencial (OE) de Piper aduncum L. sobre ovos e larvas de Haemonchus contortus, verificando se um isolado resistente (Embrapa2010), apresentaria o mesmo comportamento que um sensível (McMaster). O OE foi obtido por arraste a vapor e analisado por cromatografia para identificação dos constituintes. O óleo foi avaliado nas concentrações de 12,5 a 0,02 mg/mL no Teste de eclosão dos ovos (TEO) e nas concentrações de 3,12 a 0,01 mg/mL no Teste de desenvolvimento larvar (TDL). As concentrações inibitórias (CI) foram determinadas pelo procedimento Probit do SAS e as diferenças estatísticas geradas pela ANOVA seguida pelo teste de Tukey. Para o isolado sensível obteve-se CI 50 de 5,72 mg/mL no TEO. No TDL o óleo apresentou CI 50 e CI 90 de 0,10 mg/mL e 0,34 mg/mL para o isolado sensível e 0,22 mg/mL e 0,51 mg/mL para o resistente, respectivamente. Demonstrou-se que o OE (dilapiol 76,2%) teve alta eficácia sobre a fase L 1 . Devido às elevadas CIs obtidas para o isolado resistente, levantou-se a hipótese de que o dilapiol talvez possua um mecanismo de ação semelhante a algum grupo anti-helmíntico. O artigo faz uma revisão e discute estudos de quantificação dos constituintes majoritários do OE de P. aduncum, destacando os realizados no Brasil.

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Section: Discussioncontrasting

confidence: 55%

Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds

Gaínza

Fantatto

Chaves

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…Previous studies revealed that essential oils exert varying degrees of ovicidal and larvicidal effects against some gastrointestinal nematodes because of their chemical constituents, including linalool (Zhu et al, 2013b), 1,8-cineole (Oliveira et al, 2014;Zhu et al, 2013a), camphor (Zhu et al, 2013a), limonene (Ribeiro et al, 2013), anethole, and thymol (Camurç a-Vasconcelos et al, 2007). Borneol and ␤-elemene, the two principal constituents of ZSEO, were also evaluated in the present study.…”

Section: Tablementioning

confidence: 97%

“…Ribeiro et al (2014) showed Eucalyptus citriodora essential oils (4 mg/ml concentration) inhibited H. contortus larvae hatching by 97.2% at 4 mg/mL and inhibited larval development by 99.8% at 8 mg/mL. The essential oil from Piper aduncum showed to be effective in inhibiting H. contortus hatchability and the LC 90 was calculated as 8.9 mg/mL (Oliveira et al, 2014). Artemisia lancea essential oil at 10 mg/ mL inhibited H. contortus larvae hatching by 99.0%, inhibited larval development by 93.6%, and inhibited larval migration by 77.0% (Zhu et al, 2013a).…”

Section: Tablementioning

confidence: 99%

In vitro anthelmintic activity of Zanthoxylum simulans essential oil against Haemonchus contortus

Wang

Dai

et al. 2015

Veterinary Parasitology

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“…The authors assigned the anthelminthic effect to the eugenol, the main compound of O. gratissimum essential oil. An anthelminthic activity has also been reported for 1.8 cineole (Oliveira et al, 2014) the second major compound of O. gratissimum essential oil. Furthermore, the β-selinene is present in the essential oil of many plants and it was the third major compound of O. gratissimum oil, but no anthelminthic activity has been reported.…”

Section: Discussionmentioning

confidence: 85%

Efficacy of Ocimum gratissimum essential oil against the monogenean Cichlidogyrus tilapiae gill parasite of Nile tilapia

Meneses

Couto

Sousa

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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The phythotherapy is an alternative to use of chemotherapeutical agents against monogenean infection. This study evaluated the anthelmintic activity of essential oil Ocimum gratissimum against monogenean Cichlidogyrus tilapiae as well as its acute toxicity in tilapia juveniles. The mean lethal concentration (LC50) and different concentrations of the essential oil, both in vitro and in vivo assays (short and long-term baths) were assessed. The LC50 was 40.70mg.L-1 and in the in vitro assay this concentration showed 80% efficacy at the last two hours and in the in vivo assay 65.87% efficacy in long-term bath. However, it provoked morphological alterations on the gills such as hyperplasia and edema. The parasites exposure at the highest concentration (320mg.L-1) showed 100% mortality after 2h exposure in the in vitro assay, whereas in the in vivo assay, short-term baths of 5min for 3 consecutive days showed an efficacy of 87.71% without gills damage. These results demonstrate the anthelminthic activity of essential oil O. gratissimum and the safety concentration to use in Nile tilapia.

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Chemical composition and efficacy in the egg-hatching inhibition of essential oil of Piper aduncum against Haemonchus contortus from sheep

Cited by 26 publications

References 16 publications

Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds

Piper aduncum against Haemonchus contortus isolates: cross resistance and the research of natural bioactive compounds

In vitro anthelmintic activity of Zanthoxylum simulans essential oil against Haemonchus contortus

Efficacy of Ocimum gratissimum essential oil against the monogenean Cichlidogyrus tilapiae gill parasite of Nile tilapia

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