Assessment of HPV 16, HPV 18, p16 expression in advanced stage laryngeal cancer patients and prognostic significance

Dogantemur, Selman; Özdemir, Süleyman; Uğuz, Aysun; Sürmelioğlu, Özgür; Dagkiran, Muhammed; Tarkan, Özgür; Tuncer, Ülkü

doi:10.1016/j.bjorl.2019.11.005

Cited by 14 publications

(9 citation statements)

References 32 publications

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“…Our results support the epidemiological evidence that some head and neck cancers are caused by HPV and that the main risk factors of HNSCC are tobacco and alcohol abuse [1]. The overall prevalence of HPV in the HNSCC group was 42.1%, which is in line with a large number of reports of HPV DNA (2.4-74%) in head and neck cancer patients [8,[31][32][33]40,[50][51][52][53][54][55][56][57][58][59]. The data concerning HPV infection in HNSCC patients in Poland are very inconclusive.…”

Section: Hpv Infection In Hnsccsupporting

confidence: 89%

“…Among HPV-positive patients, the G1 grade was more prevalent than in HPV-negative subjects, and G3 was more prevalent in HPV-negative patients [ 43 ]. On the other hand, in HNSCC patients, no significant relationships were found between the presence of HPV and patients’ age, nodal status, grade, tumor stage, smoking, or alcohol consumption [ 31 , 35 , 44 , 55 , 59 , 77 ]. Our tumor samples were collected for two years.…”

Section: Discussionmentioning

confidence: 99%

“…HPV detection and identification of subtypes were performed by GenoFlow HPV (Human Papillomavirus) Array Test Kits (DiagCor Bioscience Inc., Hong Kong) which use biotin-labeled primers, specific probes, PCR and "flow-through" hybridization technology, which allows detection of 33 common HPV subtypes. The following are considered highrisk (16,18,31,33,35,39,45,51,52,53,56,58,59,66,68,73,82) and low-risk (6,11,26,40,42,43,44,54,55,57,61,70,71,72,81,84) subtypes based on their phylogenetic and epidemiological criteria and the biological niche. The extracted DNA was mixed with PCR reagent mix and DNA Taq Polymerase provided with the kit and amplified using a Mastercycler Personal Thermal Cycler (Eppendorf, Hamburg, Germany) according to the manufacturer's protocol.…”

Section: Hpv Detection and Type Determinationmentioning

confidence: 99%

“…Incidence rates for oral and oropharyngeal cancer related to HPV infections are on the increase in some countries, especially among men [9][10][11], but the global prevalence and the genotype of HPV infection in head and neck squamous cell carcinoma (HNSCC) remain obscure [8,9]. About 200 types of HPV have been identified and classified into two major groups (i.e., high-risk and low-risk subtypes), depending on their ability to trigger neoplastic transformation [1,3], 25 types of which (1,2,3,4,6,7,10,11,13,16,18,31,32,33,35,40,45,52,55,57,58,59,69,72,73) are associated with oral lesions [12].…”

Section: Introductionmentioning

confidence: 99%

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The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland

et al. 2021

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Human papillomavirus (HPV) is a virus with the potential to infect human epithelial cells and an etiological agent of many types of cancer, including head and neck cancer. The aim of the study was to determine the prevalence of HPV infection in patients with head and neck squamous cell carcinoma (HNSCC), patients with chronic tonsillitis, and healthy individuals, and to establish high- and low-risk HPV genotypes in these groups. The objectives also comprised the delineation of the relationship between the infection with high- or low-risk HPV subtypes and clinicopathological and demographic characteristics of the study groups. This study was composed of 76 patients diagnosed with HNSCC, 71 patients with chronic tonsillitis, and 168 cases without either of these conditions (the control group). HPV detection and identification of subtypes were performed on isolated DNA using a test which allowed detection of 33 common high-risk and low-risk HPV subtypes. The prevalence of HPV infection was 42.1%, 25.4%, and 37.5% in HNSCC, chronic tonsillitis, and control groups, respectively. HPV 16 was the most prevalent genotype in all groups and the non-oncogenic HPV 43/44 was frequent in HNSCC patients. This analysis provides insight into the prevalence of oral oncogenic and non-oncogenic HPVs in patients with head and neck cancer, patients with chronic tonsillitis and healthy individuals, and leads to the conclusion that further investigations are warranted to examine a larger cohort of patients focusing on high- and low-risk HPV genotypes. Efforts should be focused on screening and prevention strategies, and therefore, it is important to introduce tools for effective detection of HPV genotypes. Furthermore, given the role of vaccines against oral HPV infection, our observations lead to the suggestion that HPV vaccination should be of considerable importance in public health strategies.

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Section: Hpv Infection In Hnsccsupporting

confidence: 89%

Section: Discussionmentioning

confidence: 99%

Section: Hpv Detection and Type Determinationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland

et al. 2021

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“…For example, some research has indicated that p16 expression in LC is correlated with increased survival (14,15). However, in direct contradiction of these conclusions, others, such as Dahm and et al at the Medical University of Austria, have maintained that p16 tumor positivity does not impact survival time for these patients, instead describing smoking status and tumor staging as significant prognostic indicators (16)(17)(18).…”

Section: Introductionmentioning

confidence: 90%

The Impact of HPV DNA/p16 in Laryngeal/Hypopharyngeal Cancer: a Systematic Review and Meta-analysis

Mumaw

et al. 2021

Preprint

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Background: This meta analysis seeks to investigate the association between HPV and p16 status with overall survival in laryngeal and hypopharyngeal carcinoma. Methods: Medline, Scopus, EMBASE, and the Cochrane Library were used to identify studies for inclusion. Abstracts that discussed HPV/p16 status and prognosis in laryngeal or hypopharyngeal carcinoma were included. Next, full text articles were screened and included based upon a checklist established a priori. Pooled hazard ratios for overall survival were generated using a random effects model. RevMan 5.3, Meta Essentials, and OpenMeta[Analyst] were used for statistical analysis. Results: Thirteen studies published between 2014 and 2019 with sample sizes ranging from 31 to 9,656 were selected for inclusion in this meta analysis. The pooled data demonstrated that p16 status was not significantly associated with OS in either laryngeal or hypopharyngeal carcinoma with HRs of 1.03 (95% CI: 0.73, 1.45; p = 0.88) and 1.02 (95% CI: 0.55, 1.86; p = 0.96), respectively. The pooled data showed that HPV status was predictive of OS in laryngeal cancer with 0.63 (95% CI: 0.41, 0.97; p = 0.03). Conclusions: Our results suggest that p16-positivity does not provide a survival benefit in LC and HPC. This is in contrast to studies in the oropharynx, where p16 status is a standard proxy for HPV infection and HPV infection is associated with improved prognosis.

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HPV 16 E6 promotes growth and metastasis of esophageal squamous cell carcinoma cells in vitro

Miao

et al. 2022

Mol Biol Rep

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BackgroundEsophageal squamous cell carcinoma (ESCC) is one of the most lethal malignancies. Increasing evidence has revealed that Human Papillomavirus (HPV) infection may be associated with the possible etiology of ESCC. Nevertheless, the precise role of HPV in ESCC remains unclear. MethodsProliferation and apoptosis capability of ESCC cells upon infection of HPV16 E6 were detected by CCK-8 assay and Western blotting analysis. Wound healing assay and Transwell experiment were conducted to determine the ability of migration and invasion, and the mRNA expression of E6AP, p53, miR-34a was determined by real-time PCR after transfected with HPV16 E6 plasmid. ResultsIn ESCC cells, the ability of proliferation, migration and invasion were increased, and the ability of apoptosis was decreased after transfected with HPV16 E6 plasmid. Furthermore, the mRNA level of E6AP, P53 and miR-34a were decreased in HPV16 E6-transfected cell lines. ConclusionsOur results not only provide evidence that in ESCC, HPV16 E6 promotes cell proliferation, migration and invasion, inhibits cell apoptosis, but also suggest that there may be a correlation between E6AP, P53 and miR-34a in HPV-transfected cell lines. These ndings indicated that HPV16 E6 may play an important role in the development of ESCC.

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Assessment of HPV 16, HPV 18, p16 expression in advanced stage laryngeal cancer patients and prognostic significance

Cited by 14 publications

References 32 publications

The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland

The Prevalence of High- and Low-Risk Types of HPV in Patients with Squamous Cell Carcinoma of the Head and Neck, Patients with Chronic Tonsillitis, and Healthy Individuals Living in Poland

The Impact of HPV DNA/p16 in Laryngeal/Hypopharyngeal Cancer: a Systematic Review and Meta-analysis

HPV 16 E6 promotes growth and metastasis of esophageal squamous cell carcinoma cells in vitro

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