The influence of growth factors on skin wound healing in rats

Masi, Elen Carolina David João De; Campos, Antônio Carlos Ligocki; Masi, Flávia David João De; Ratti, Marco Aurelio Soatti; Ike, Isabela Shin; Masi, Roberta David João De

doi:10.1016/j.bjorl.2015.09.011

Cited by 45 publications

(31 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Platelets play the most important role at this stage through the formation of a blood clot (coagulation cascade) and the production of multiple signaling molecules such as PDGF, EGF, fibronectin, fibrinogen, histamine, serotonin and the von Willebrand factor. 16,17 PDGFs are released from the alpha granules of platelets thus promoting mitogenicity and the chemotaxis of the neutrophils, macrophages, fibroblasts and smooth muscle cells at the wound site. 18 The increased infiltration of neutrophils, mast cells, monocytes and T lymphocytes into the wound site occurs during the inflammation stage, 19 and TGF-β, TNF-α, EGF, PDGF, VEGF, FGF, IL-1, IL-6, IL-8 and IL-12 are all present in the wound environment at this phase of healing.…”

Section: Cutaneous Wound Healingmentioning

confidence: 99%

<p>The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism</p>

Lasocka¹,

Jastrzębska²,

Szulc-Dąbrowska³

et al. 2019

IJN

View full text Add to dashboard Cite

This study provides a review of the therapeutic potential of graphene dressing scaffolds and mesenchymal stem cells (MSCs) and their synergistic effects with respect to cutaneous wound healing. This study also considers their putative action mechanism based on the antibacterial, immunomodulating, angiogenic, matrix remodeling effects of materials belonging to the graphene family and MSCs during the wound healing process. In addition, this study discusses the cytocompatibility of graphene, its uses as a platform for skin substitutes, the properties it possesses with respect to providing protection against microbial invasion as well as strategies aimed at minimizing the chance of the occurrence of sepsis. MSCs are capable of secreting several factors that exert a therapeutic impact on reparative processes and tissue regeneration. In light of experiments conducted to date, graphene combined with MSCs appears to have the potential to enhance both the wound healing process and infection control at the injury site.

show abstract

Section: Cutaneous Wound Healingmentioning

confidence: 99%

<p>The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism</p>

Lasocka¹,

Jastrzębska²,

Szulc-Dąbrowska³

et al. 2019

IJN

View full text Add to dashboard Cite

show abstract

“…[4] Radiation-induced delayed wound recovery is attributed to the downregulation of angiogenic growth factors such as vascular endothelial growth factor (VEGF), transforming growth factor and fibroblast growth factor in the wound. [4][5][6] Histopathological characteristics of impaired wound healing after irradiation include defective neovascularization in both animal models and human patients. [7,8] Therefore, endothelial dysfunction may be a key mechanism of radiation-induced tissue damage.…”

Section: Introductionmentioning

confidence: 99%

Platelet‐rich plasma improves the therapeutic efficacy of mesenchymal stem cells by enhancing their secretion of angiogenic factors in a combined radiation and wound injury model

Myung

Jang

Myung

et al. 2019

Experimental Dermatology

View full text Add to dashboard Cite

Delayed wound healing after radiation exposure can cause serious cutaneous damage, and its treatment is a major clinical challenge. Although mesenchymal stem cells (MSCs) have emerged as a promising therapeutic agent in regenerative medicine, they alone do not produce satisfactory effects in a combined radiation and wound injury (CRWI) model. Here, we investigated the therapeutic effect of combined umbilical cord blood‐derived (UCB)‐MSCs and platelet‐rich plasma (PRP) treatment on wound healing in a CRWI mouse model. First, we assessed the release of cytokines from UCB‐MSCs cultured with PRP and observed changes in the expression of angiogenic factors. The angiogenic paracrine factors from UCB‐MSCs cultured with PRP were assessed in human umbilical vein endothelial cells (HUVECs). To assess therapeutic efficacy, UCB‐MSCs and PRP were topically implanted into a CRWT mouse model. Vascular endothelial growth factor (VEGF), a pro‐angiogenic growth factor, urokinase‐type plasminogen activator and contributor to VEGF‐induced signalling were more highly expressed in conditioned media of UCB‐MSCs cultured with PRP than in that of UCB‐MSCs alone. Furthermore, conditioned media of UCB‐MSCs cultured with PRP increased the formation of tube‐like structures in HUVECs. Co‐treatment of UCB‐MSCs and PRP in a CRWI mouse model increased the wound closure rate and angiogenesis compared with an untreated irradiated group. Moreover, increased expression of VEGF and CD31 were observed in the wound tissue of co‐treated mice compared with untreated irradiated mice. PRP stimulates the release of angiogenic factors from UCB‐MSCs, and combined therapy of UCB‐MSCs and PRP improves regeneration efficacy by enhancing angiogenesis in a CRWI model.

show abstract

“…However, radiation may also directly act on skin itself, by inhibiting DNA synthesis, prolonging cell cycle, disturbing the proliferation of endotheliocytes and fibroblasts, decreasing the neovascularization and collagen synthesis, and finally delay wound repair and remodelling . Otherwise, radiation‐induced delayed wound recovery can be attributed to the downregulation of some growth factors, such as vascular endothelial growth factor (VEGF), transforming growth factor (TGF) and fibroblast growth factor (FGF) secreted by certain cells in the wound . Therefore, radiation can systemically or locally blunt wound healing.…”

Section: Introductionmentioning

confidence: 99%

“…[13] Otherwise, radiation-induced delayed wound recovery can be attributed to the downregulation of some growth factors, such as vascular endothelial growth factor (VEGF), transforming growth factor (TGF) and fibroblast growth factor (FGF) secreted by certain cells in the wound. [14][15][16][17] Therefore, radiation can systemically or locally blunt wound healing.…”

mentioning

confidence: 99%

Ghrelin accelerates wound healing in combined radiation and wound injury in mice

Liu

Hao

Huang

et al. 2017

Experimental Dermatology

View full text Add to dashboard Cite

Impaired wound healing caused by radiation happens frequently in clinical practice, and the exact mechanisms remain partly unclear. Various countermeasures have been taken to tackle with this issue. Ghrelin was considered as a potent endogenous growth hormone-releasing peptide, and its role in enhancing wound repair and regeneration was firstly investigated in whole-body irradiated (γ-ray) mice in this study. Collagen deposition and neovascularization were mostly discussed. The results demonstrated that ghrelin administration promoted cutaneous wound healing in irradiated mice, followed with reduced average wound closure time, increased spleen index (SI) and improved haematopoiesis. After isolation and analysis of granulation tissues in combined radiation and wound injury (CRWI) mice treated with and without ghrelin, a phenomenon of increased DNA, hexosamine, nitrate and nitrite synthesis, elevated collagen content and enhanced neovascularization was observed after ghrelin treatment. Western blotting indicated that ghrelin also increased the expression of vascular endothelial growth factor (VEGF) and transforming growth factor-β (TGF-β), both responsible for wound healing. However, previous administration of growth hormone secretagogue receptor 1a (GHS-R1a) blocker blunted these therapeutic effects of ghrelin on CRWI mice. Our results identify ghrelin as a novel peptide that could be used for radiation-induced impaired wound healing.

show abstract

The influence of growth factors on skin wound healing in rats

Abstract: The use of growth factors accelerates healing, stimulates greater angiogenic activity, and accelerates fibroplasia and collagen maturation.

Cited by 45 publications

References 20 publications

<p>The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism</p>

<p>The effects of graphene and mesenchymal stem cells in cutaneous wound healing and their putative action mechanism</p>

Platelet‐rich plasma improves the therapeutic efficacy of mesenchymal stem cells by enhancing their secretion of angiogenic factors in a combined radiation and wound injury model

Ghrelin accelerates wound healing in combined radiation and wound injury in mice

Contact Info

Product

Resources

About