2018
DOI: 10.1016/j.bjm.2018.06.002
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Influence of iron and copper on the activity of laccases in Fusarium oxysporum f. sp. lycopersici

Abstract: Fusarium oxysporum f. sp. lycopersici is a phytopathogenic fungus that causes vascular wilt in tomato plants. In this work we analyze the influence of metal salts such as iron and copper sulphate, as well as that of bathophenanthrolinedisulfonic acid (iron chelator) and bathocuproinedisulfonic acid (copper chelator) on the activity of laccases in the intra (IF) and extracellular fractions (EF) of the wild-type and the non-pathogenic mutant strain (rho1::hyg) of F. oxysporum. The results show that laccase activ… Show more

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Cited by 13 publications
(8 citation statements)
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“…In this regard, addition of CuSO 4 to fungus cultures resulted in a substantial increase in laccase activity [61]. Notwithstanding, there is also evidence of a possible decrease in enzyme activity due to the presence of copper salts [62]. Here, however, we disregarded possible effects of Cu +2 on the activity of encapsulated laccase molecules.…”
Section: Discussionmentioning
confidence: 99%
“…In this regard, addition of CuSO 4 to fungus cultures resulted in a substantial increase in laccase activity [61]. Notwithstanding, there is also evidence of a possible decrease in enzyme activity due to the presence of copper salts [62]. Here, however, we disregarded possible effects of Cu +2 on the activity of encapsulated laccase molecules.…”
Section: Discussionmentioning
confidence: 99%
“…It is also suggested that the presence of polyols in the laccase reaction media act as a water-structure maker which depresses the hydration of the enzyme and hence its denaturation (Papinutti et al 2008 ). Although the metal ions including copper play crucial role as enhancers of laccase activity, especially that laccases have four copper atoms in their catalytic center, it could inhibit it at varying degrees, yet the destabilizing mechanism was not elucidated yet (Hernandez-Monjoraz et al ( 2018 ).…”
Section: Discussionmentioning
confidence: 99%
“…También el cobre tipo I participa en el sitio activo donde se lleva a cabo la oxidación de los sustratos, y el cobre tipo III es donde ocurre la reducción del oxígeno molecular hasta agua [45]. Son diversos los factores que pueden inducir la síntesis de LAC, pero teniendo en cuenta el rol que cumplen los átomos de cobre y la estructura de esta ligninasa, se ha sugerido que la adición de algunos metales en los medios de cultivo influye sobre el proceso de transcripción genética incrementando la producción de esta enzima [46,47,48]. Existe evidencia científica de la presencia de promotores sensibles a metales en genes de enzimas ligninolíticas, lo cual indica que la presencia de algunos metales como cobre y manganeso en el medio de cultivo pueden actuar estabilizando o activando el procesamiento y tráfico de la información genética del ARN mensajero que codifica la información para la síntesis de una nueva proteína [8,20,49,50].…”
Section: Efecto De La Adición De Cobre Manganeso Y Calcio Sobre La Producción De Ligninasas Y Xilanasaunclassified