The potential of the newly isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 for high-temperature ethanol production

Chamnipa, Nuttaporn; Thanonkeo, Sudarat; Klanrit, Poramate; Thanonkeo, Pornthap

doi:10.1016/j.bjm.2017.09.002

Cited by 65 publications

(57 citation statements)

References 84 publications

(118 reference statements)

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“…HSPs are expressed constitutively at high temperatures to assist with folding and assembly of proteins, refolding of damaged proteins, stabilization of new proteins and transport across the membranes. Some display proteolytic activity, p r e v e n t i n g d e n a t u r e d p r o t e i n s f r o m accumulating in the cells (Chamnipa et. al., 2018).…”

Section: Discussionmentioning

confidence: 99%

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Preliminary evaluation of sensitivity of <i>Saccharomyces cerevisiae</i> LC 269108 to thermal and ethanol stresses in fermentation of agro-wastes for bioethanol production

Nwuche¹

2019

Nig. J. Biotechnol

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The effect of two temperature regimes and ethanol accretion on the ethanolegenic characteristic of Saccharomyces cerevisiae LC 269108 was investigated in shaker flask experiments using flour processed from dried yam peels as feedstock. The flour was initially liquefied in boiling water before enzymatic pretreatment using amyloglucosidase, cellulase and pectinase. To 20% (w/v) of the gelatinized syrup was added 0.5 ml of nitrogen base and 1 ml of standardized overnight suspension of the culture. Fermentation was carried out for 60 h at 120 rpm, pH of 5.5 and temperature of 30 and 40 °C. A 5% (v/v) ethanol was included in a separate batch and fermented as described. Results show that ethanol concentration increased at both temperature conditions as well as in the batch supplemented with ethanol. Glucose decreased from initial concentration of 9.63 ± 0.34% until it was completely exhausted from the system. At 30 °C, peak concentration of ethanol (5.30 ± 0.2%) was achieved after 24 h. However, at 40 °C the highest ethanol content (5.61 ± 0.57%) was reached after 48 h. No difference (p < 0.05) was found between the concentration of ethanol produced at the two temperature states. In the batch augmented with ethanol (5%), peak production (4.41 ± 0.20%) was reached after 24 h at both 30 and 40 °C. Subsequently, ethanol concentration maintained plateau at 30 °C but in the batch incubated at 40 °C, it dropped steadily after 24 h until reaching final concentration of 3.93 ± 0.25% after 60 h. The yeast displayed promising attributes which can be harnessed for future developments in high temperature ethanol fermentation.

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Section: Discussionmentioning

confidence: 99%

“…Yeasts have traditionally been used in microbial fermentations for the production of ethanol. Many factors such as heat, ethanol, osmotic pressure and feedstock breakdown products (Chamnipa et. al., 2018) negatively affect the specific growth rate of many yeasts thereby lowering ethanol production.…”

Section: Isolation and Characteristics Of The Yeast Strainmentioning

confidence: 99%

Preliminary evaluation of sensitivity of <i>Saccharomyces cerevisiae</i> LC 269108 to thermal and ethanol stresses in fermentation of agro-wastes for bioethanol production

Nwuche¹

2019

Nig. J. Biotechnol

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“…Ethanol production at high temperatures by thermotolerant yeasts has earned much interest due to several advantages as described above [38]. There are several ethanologenic yeasts that have been characterized and classified as thermotolerant yeasts such as K. marxianus [31,37,47], P. kudriavzevii (formally known as I. orientalis) [20,48,49,65,66], Hansenula polymorpha [67], and some strains of S. cerevisiae [21,52,[68][69][70]. However, for cost-effective and efficient ethanol production, not only thermotolerance but also a broad spectrum in sugar assimilation and fermentation capability is beneficial for the conversion of a variety of raw materials containing various sugars to ethanol, especially xylose, which is the most common pentose sugar and the second most abundant after glucose in lignocellulosic biomass and algal biomass [71,72].…”

Section: Utilization Of Various Sugars In Thermotolerant Yeastsmentioning

confidence: 99%

“…The strain KVMP10 that was isolated from soil located beneath apple trees for ethanol production from orange peel achieved 54 g/L ethanol at 42°C [48]. Strain RZ8-1 that was recently isolated from various samples collected from plant orchards in Thailand produced 33.8 g/L ethanol from 160 g/L glucose at 40°C [49].…”

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confidence: 99%

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

Kosaka¹,

Lertwattanasakul²,

NadchanokRodrussamee³

et al. 2019

Fuel Ethanol Production From Sugarcane

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Thermotolerant ethanologenic yeasts receive attention as alternative bio-ethanol producers to traditionally used yeast, Saccharomyces cerevisiae. Their utilization is expected to provide several benefits for bio-ethanol production due to their characteristics and robustness. They have been isolated from a wide variety of environments in a number of ASEAN countries: Thailand, Vietnam, Laos, and Indonesia. One of these yeasts, Kluyveromyces marxianus has been investigated regarding characteristics. Some strains efficiently utilize xylose, which is a main component of the 2nd generation biomass. In addition, the genetic basis of K. marxianus has been revealed by genomic sequencing and is exploited for further improvement of the strains by thermal adaptation or gene engineering techniques. Moreover, the glucose repression of K. marxianus and its mechanisms has been investigated. Results suggest that K. marxianus is an alternative to S. cerevisiae in next-generation bio-ethanol production industry. Indeed, we have succeeded to apply K. marxianus for bio-ethanol production in a newly developed process, which combines high-temperature fermentation with simultaneous fermentation and distillation under low pressure. This chapter aims to provide valuable information on thermotolerant ethanologenic yeasts and their application, which may direct the economic bioproduction of ethanol and other useful materials in the future.Recently, thermotolerant microorganisms were found among mesophiles with optimum growth temperatures that are 5-10°C higher than those of the typical mesophilic strains Fuel Ethanol Production from Sugarcane 122 Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries… http://dx.doi.org/10.5772/intechopen.79144 123Recently, there have been several reports on ethanol production at high temperatures using P. kudriavzevii (formerly known as I. orientalis). Several P. kudriavzevii strains were reported to grow and produce high levels of ethanol at high temperatures. The strain DMKU 3-ET15 was isolated from traditional fermented pork sausage in Thailand by an enrichment technique in a medium supplemented with 4% ethanol at 40°C. The strain produced 78.6 g/L ethanol from 180 g/L glucose at 40°C [20]. The strain KVMP10 that was isolated from soil located beneath apple trees for ethanol production from orange peel achieved 54 g/L ethanol at 42°C [48]. Strain RZ8-1 that was recently isolated from various samples collected from plant orchards in Thailand produced 33.8 g/L ethanol from 160 g/L glucose at 40°C [49].

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“…A habilidade para fermentação das pentoses presentes no hidrolisado ainda não é difundida entre os organismos, ao passo que a fermentação de todos os açúcares liberados na hidrólise é determinante para aumentar o rendimento do processo (CHAMNIPA et al, 2017). Graças aos avanços em engenharia genética linhagens de S. cerevisiae capazes de assimilar pentoses e hexoses vêm sendo desenvolvidas.…”

Section: Introductionunclassified

FERMENTAÇÃO ALCOÓLICA DE HIDROLISADO DE BAGAÇO DE CANA UTILIZANDO Saccharomyces cerevisiae RECOMBINANTE IMOBILIZADA

Pérez¹,

Sandri²,

Mesquita³

et al. 2018

Blucher Chemical Engineering Proceedings

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RESUMO -O efeito de diferentes concentrações de etanol e da presença de inibidores sobre a fermentação de xilose e glicose foi avaliado em experimentos conduzidos em meio complexo (YPDX) e em hidrolisado de bagaço de cana de açúcar (HB). As células de levedura recombinante foram imobilizadas em alginato de cálcio e o efeito protetor do alginato a inibidores presentes no meio HB foi constatado. Por outro lado, a exposição a altas concentrações de etanol por tempo prolongado causou a perda de viabilidade das células, mesmo imobilizadas, a partir da segunda batelada repetida.

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The potential of the newly isolated thermotolerant yeast Pichia kudriavzevii RZ8-1 for high-temperature ethanol production

Cited by 65 publications

References 84 publications

Preliminary evaluation of sensitivity of <i>Saccharomyces cerevisiae</i> LC 269108 to thermal and ethanol stresses in fermentation of agro-wastes for bioethanol production

Preliminary evaluation of sensitivity of <i>Saccharomyces cerevisiae</i> LC 269108 to thermal and ethanol stresses in fermentation of agro-wastes for bioethanol production

Potential of Thermotolerant Ethanologenic Yeasts Isolated from ASEAN Countries and Their Application in High- Temperature Fermentation

FERMENTAÇÃO ALCOÓLICA DE HIDROLISADO DE BAGAÇO DE CANA UTILIZANDO Saccharomyces cerevisiae RECOMBINANTE IMOBILIZADA

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