Comparative study of RFLP-IS6110 and MIRU-VNTR from Mycobacterium tuberculosis isolated in the state of Minas Gerais, Brazil

Abstract

DNA genotyping of Mycobacterium tuberculosis has been widely applied in the understanding of disease transmission in many countries. The purpose of this study was to genotype the strains of M. tuberculosis isolated in patients with new tuberculosis (TB) cases in Minas Gerais, as well as to compare the similarity, discriminatory power, and agreement of the clusters between the IS6110 Restriction Fragment Length Polymorfism (RFLP) and 12 loci Variable Number Tandem Repeat – Mycobacterial Interspersed Repetitive Units (MIRU-VNTR) techniques. It was observed that 32% (66/204) of the isolated strains in the RFLP-IS6110 and 50.9% (104/204) of the isolated strains in the MIRU-VNTR presented a similarity of equal to or above 85%. The RFLP-IS6110 and MIRU-VNTR proved to contain a high discriminatory power. The similarity index resulting from the RFLP showed no recent transmission. Good agreement was observed between the techniques when clusters were detected; however, the best epidemiological relationship was found when using the RFLP-IS6110.

Usefulness of 3’- 5’ IS6110-RFLP genotyping and spoligotyping of Mycobacterium tuberculosis isolated in a tertiary hospital: a retrospective study detecting unsuspected epidemiological events

“…31 reported 3.1% of mixed infections by combining RFLP and spoligotyping in a rural area of Vietnam, equal frequency of our study, using both methods. Another study carried out a retrospective genotyping in previously samples suspected of having epidemiological relationships 32 , which in contrary to the present study, they described a slightly more adequate RFLP cluster results compared to MIRU-VNTR 32 , 33 . Additionally, RFLP lacks the discriminatory power for strains presenting less than six banding patterns, while spoligotyping and MIRU-VNTR are more adequate in this regard.…”

Exploring the “Latin American Mediterranean” family and the RDRio lineage in Mycobacterium tuberculosis isolates from Paraguay, Argentina and Venezuela

“…Furthermore, based on the allelic diversity, there is previous evidence suggesting that the number and combination of loci to be used to discriminate between genotypes can be adapted to the population of Mtb strains [46–48]. In this study, applying 12 loci MIRU [49, 50] allowed us to detect the existence of clusters with members of the LAM, H or S families respectively, with the presence of T isolates inserted in between. Interestingly, when evaluating conflicting genotypes, Ag85C SNP and Spotclust classification showed to be useful to solve the issues.…”

Molecular epidemiology of Mycobacterium tuberculosis in Brazil before the whole genome sequencing era: a literature review

“…In the case of MIRU-VNTR data, some articles were excluded because they only contained information on M. tuberculosis var. bovis (n = 5); 76 , 77 , 78 , 79 , 80 no data on genotyping were available (n = 6); 49 , 66 , 81 , 82 , 83 , 84 incomplete information on genetic diversity (n = 3) 85 , 86 , 87 or genotyping data is mixed with samples from other countries (n = 3). 84 , 88 , 89 …”