Mangrove endophyte promotes reforestation tree ( Acacia polyphylla ) growth

Abstract

Mangroves are ecosystems located in the transition zone between land and sea that serve as a potential source of biotechnological resources. Brazil's extensive coast contains one of the largest mangrove forests in the world (encompassing an area of 25,000 km2 along all the coast). Endophytic bacteria were isolated from the following three plant species: Rhizophora mangle, Laguncularia racemosa and Avicennia nitida. A large number of these isolates, 115 in total, were evaluated for their ability to fix nitrogen and solubilize phosphorous. Bacteria that tested positive for both of these tests were examined further to determine their level of indole acetic acid production. Two strains with high indole acetic acid production were selected for use as inoculants for reforestation trees, and then the growth of the plants was evaluated under field conditions. The bacterium Pseudomonas fluorescens (strain MCR1.10) had a low phosphorus solubilization index, while this index was higher in the other strain used, Enterobacter sp. (strain MCR1.48). We used the reforestation tree Acacia polyphylla. The results indicate that inoculation with the MCR1.48 endophyte increases Acacia polyphylla shoot dry mass, demonstrating that this strain effectively promotes the plant's growth and fitness, which can be used in the seedling production of this tree. Therefore, we successfully screened the biotechnological potential of endophyte isolates from mangrove, with a focus on plant growth promotion, and selected a strain able to provide limited nutrients and hormones for in plant growth.

The Effect of Microbial Endophyte Consortia on Pseudotsuga menziesii and Thuja plicata Survival, Growth, and Physiology Across Edaphic Gradients

“…The role of endophytes in forest communities remains poorly understood; however, high-throughput technologies have increased the speed and resolution in screening microbial communities (Hardoim et al, 2015; Baldrian, 2017). Endophyte inoculated trees have shown: (1) positive growth trends (Chanway and Holl, 1991; Chanway et al, 1994; Shishido et al, 1999; Khan et al, 2015; Castro et al, 2017), (2) adaptive phenotypic changes (Knoth et al, 2014; Vivas et al, 2015), (3) stress mitigation (Khan et al, 2016; Doty, 2017; Doty et al, 2017; Rho et al, 2018a, b), (4) nitrogen fixation (Anand and Chanway, 2013; Anand et al, 2013; Knoth et al, 2014), and (5) reduced damage from insect herbivores and fungal pathogens (Brooks et al, 1994; Miller, 2011; Pirttilä and Frank, 2011).…”

In Vitro Characterization of Endophytic Bacteria Associated With Physic Nut (Jatropha Curcas L.) and Their Potential for Plant-Growth Promotion and Biocontrol / Caracterização in Vitro De Bactérias Endofíticas Associadas Ao Pinhão-Manso (Jatropha Curcas L.) E Seu Potencial De Promoção De Crescimento Vegetal E Biocontrole

“…Some studies of the endophytic and rhizospheric bacteria diversity associated with J. curcas found the phyla Actinobacteria, Acidobacteria, Chlroflexi, Firmicutes, and Verrucomicrobia Annapurna;Saraf, 2012;Dubey et al 2016;Madhaiyan et al, 2012;Qin et al, 2015); which demonstrates the ability of the phyla found in the present study in Annapurna;Saraf, 2012;Madhaiyan et al, 2012;Madhaiyan et al, 2013). These genera have already been described as capable of promoting plant growth in various other crops (Chauhan, Bagyaraj, Sharma, 2013;Castro et al, 2017;Batista et al, 2018).…”

Surface elevation change evaluation in mangrove forests using a low‐cost, rapid‐scan terrestrial laser scanner

“…This was attributed to the specifications of the TLS they used, the Faro Focus 120 3D phase‐shift TLS, and also the structural attributes of the study site. The Faro Focus 120 3D phase‐shift TLS provides much denser data when compared to the CBL, being able to collect up to 976,000 points per scan (Castro et al, 2018), while the CBL collects up to approximately 300,000 points per scan in natural environments. In addition, the beam divergence in the Faro Focus 120 3D is 0.19 mrad, which provides a higher resolution compared to the 15‐mrad beam divergence of the CBL, while the range accuracy for the Faro Focus 120 3D is as low as 2 mm (at ranges < 120 m), while the range accuracy for the CBL is specified as 30 mm (at ranges < 50 m) (Kelbe et al 2015).…”