Complete genome sequence of human pathogen Kosakonia cowanii type strain 888-76 T

Yang, Xuejing; Wang, Qianqian; Cao, Junhui; Hou, Jiahui

doi:10.1016/j.bjm.2017.03.010

Cited by 21 publications

(10 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Species identification was confirmed by partial sequencing of the 16S ribosomal RNA (rRNA) gene, which showed 100% identity of non-ambiguous bases to the 16S rRNA of K. cowanii type strain 888-76 (GenBank accession number CP019445.1) [4]. Notably, biochemical specieslevel identification, performed with VITEK2 GN ID card (Biomerieux, Marcy-l'Etoile, France), incorrectly identified the strain as Pantoea spp.…”

Section: Microbiologymentioning

confidence: 99%

Identification of Kosakonia cowanii as a rare cause of acute cholecystitis: case report and review of the literature

et al. 2020

View full text Add to dashboard Cite

Background: Kosakonia cowanii, formerly known as Enterobacter cowanii, is a Gram-negative bacillus belonging to the order Enterobacterales. The species is usually recognized as a plant pathogen and has only anecdotally been encountered as a human pathogen. Here we describe the rare case of a K. cowanii infection presenting as an acute cholecystitis and provide a review of available literature. Evident difficulties in species identification by biochemical profiling suggests that potentially, K. cowanii might represent an underestimated human pathogen. Case presentation: A 61-year old immunocompromised man presented to the hospital with fever and pain in the upper right abdomen. Sonography revealed an inflamed gall bladder and several gall stones. A cholecystectomy proved diagnosis of an acute cholecystitis with a partial necrosis of the gall bladder. Surgical specimen grew pure cultures of Gram-negative rods unambiguously identified as K. cowanii by MALDI-TOF, 16S-rRNA analysis and whole genome sequencing. Conclusions: Reporting cases of Kosakonia species can shed light on the prevalence and clinical importance of this rare cause of human infection. Our case is the first to describe an infection without prior traumatic inoculation of the pathogen from its usual habitat, a plant, to the patient. This raises the question of the route of infections as well as the pathogen's ability to colonize the human gut.

show abstract

Section: Microbiologymentioning

confidence: 99%

Identification of Kosakonia cowanii as a rare cause of acute cholecystitis: case report and review of the literature

et al. 2020

View full text Add to dashboard Cite

show abstract

“…Its closely related strain, K. oryzae, is also revealed to induce auxin and produce siderophores which are primarily responsible for the plants' nutrition and immunity against diseases (de Souza et al, 2012). However, like the Staphylococcus spp., this isolate is also a human pathogen first to be isolated in the blood of a human patient (Yang et al, 2018).…”

Section: Molecular Identification Of Endophytic Bacteria By 16s Rrna mentioning

confidence: 96%

Evaluation of the biocontrol potential of endophytic bacteria isolated from Coffea liberica (w. Bull ex hiern) against brown eyespot-causing fungal phytopathogen

Lirio¹,

Suavengco²,

Antonio³

et al. 2020

MJM

View full text Add to dashboard Cite

Aims: The study aims to investigate the potential of the endophytic bacteria as an alternative to control the devastating brown eyespot disease caused by Cercospora sp. in coffee plants. The fungal phytopathogen causes severe leaf fall and berry damages resulting in serious yield losses in coffee farms in the Philippines and worldwide. Currently, the management of this fungal infection relies heavily on synthetic fungicides, which may be of major environmental concern. Methodology and results: Endophytic bacteria were isolated from the intercellular tissues of Coffea liberica leaves by surface sterilization, maceration, dilution technique, plating on trypticase soy agar and colony characterization. Fourteen isolated endophytic bacteria were screened for their ability to inhibit the mycelial growth of Cercospora sp. through modified dual culture assay. Isolates HCC10-3 SC3, HCC10-3 SC2, HCC10-1 SC1, ICC10-3 SC1, and ICC10-1 SC1 yielded the highest percent inhibition of radial growth (PIRG) with 59.56%, 60.92%, 60.96%, 64.36%, and 67.06% respectively and are statistically significant (p<0.05) compared to the antibiotic control nystatin. The top five performing endophytic bacteria were subjected to hydrolytic enzyme production assays and found to exhibit amylolytic, lipolytic, proteolytic, chitinolytic, and cellulolytic activities. Based on the morphological and molecular identification by the 16S rRNA sequence analysis, isolates showed the similarity with Staphylococcus cohnii, Bacillus siamensis, Staphylococcus hominis, and Kosakonia cowanii found in GENBANK. Conclusion, significance and impact of study: The study revealed the biological control potential of endophytic bacteria agents against the brown eyespot-causing fungus in coffee.

show abstract

“…Most Kosakonia species are isolated from soil or trees, and several of them can promote plant growth. However, some other Kosakonia species, such as Kosakonia cowanii, are associated with human beings (1, 2). To date, several genera of bacteria have been reported to produce fucose-containing exopolysaccharides, while no literature has reported the production of fucose-containing polysaccharides by Kosakonia strains.…”

Section: Announcementmentioning

confidence: 99%

Complete Genome Sequence of Kosakonia sp. Strain CCTCC M2018092, a Fucose-Rich Exopolysaccharide Producer

Niu

Jin

et al. 2019

Microbiol Resour Announc

View full text Add to dashboard Cite

Kosakonia sp. strain CCTCC M2018092 is a fucose-rich exopolysaccharide producer that was isolated from spring water in Chongqing, Southwest China. In this study, the whole-genome sequence and genetic characteristics of this strain were elucidated. This genome contained 4,789,478 bp, with a G+C content of 56.08% (excluding the plasmid). The genome information in this study will facilitate understanding of the mechanism of high yield of fucose-rich exopolysaccharide produced by Kosakonia sp.

show abstract

Complete genome sequence of human pathogen Kosakonia cowanii type strain 888-76 T

Cited by 21 publications

References 10 publications

Identification of Kosakonia cowanii as a rare cause of acute cholecystitis: case report and review of the literature

Identification of Kosakonia cowanii as a rare cause of acute cholecystitis: case report and review of the literature

Evaluation of the biocontrol potential of endophytic bacteria isolated from Coffea liberica (w. Bull ex hiern) against brown eyespot-causing fungal phytopathogen

Complete Genome Sequence of Kosakonia sp. Strain CCTCC M2018092, a Fucose-Rich Exopolysaccharide Producer

Contact Info

Product

Resources

About