Variation analysis of bacterial polyhydroxyalkanoates production using saturated and unsaturated hydrocarbons

Abstract

Polyhydroxyalkanoates (PHA) are efficient, renewable and environment friendly polymeric esters. These polymers are synthesized by a variety of microbes under stress conditions. This study was carried out to check the suitability of waste frying oil in comparison to other oils for economical bioplastic production. Six bacterial strains were isolated and identified as Bacillus cereus (KF270349), Klebsiella pneumoniae (KF270350), Bacillus subtilis (KF270351), Brevibacterium halotolerance (KF270352), Pseudomonas aeruginosa (KF270353), and Stenotrophomonas rhizoposid (KF270354) by ribotyping. All strains were PHA producers so were selected for PHA synthesis using four different carbon sources, i.e., waste frying oil, canola oil, diesel and glucose. Extraction of PHA was carried out using sodium hypochlorite method and maximum amount was detected after 72 h in all cases. P. aeruginosa led to maximum PHA production after 72 h at 37 °C and 100 rpm using waste frying oil that was 53.2% PHA in comparison with glucose 37.8% and cooking oil 34.4%. B. cereus produced 40% PHA using glucose as carbon source which was high when compared against other strains. A significantly lesser amount of PHA was recorded with diesel as a carbon source for all strains. Sharp Infrared peaks around 1740–1750 cm−1 were present in Fourier Transform Infrared spectra that correspond to exact position for PHA. The use of waste oils and production of poly-3hydroxybutyrate-co-3hydroxyvalerate (3HB-co-3HV) by strains used in this study is a good aspect to consider for future prospects as this type of polymer has better properties as compared to PHBs.

Forest soil bacteria able to produce homo and copolymers of polyhydroxyalkanoates from several pure and waste carbon sources

“…These results have not been reported previously for S. rhizophila, although the production of PHB by related strains (S. maltophila and S. rhizoposid) has been mentioned from glucose, starch-wastewater and waste oil (11Á7, 10Á4 and 5Á7 g per 100 g DCW, respectively) (Iqbal et al 2016;Tufail et al 2017).…”

Microbial valorization of waste cooking oils for valuable compounds production – a review

“…Several bacterial species are capable to accumulate PHAs intracellularly from WCO (Table 1), including Pseudomonas sp., Cupriavidus necator, Klebsiella pneumonia, Bacillus sp., Burkholderia thailandensis, among others (Benesova et al, 2017;Kamilah et al, 2018;Kourmentza et al, 2018;Lukasiewicz et al, 2018;Tufail et al, 2017). However, some naturally PHAstoring bacteria are not able of hydrolyzing triglycerides to release free fatty acids, due to the lack of an effective lipolytic activity.…”

Effect of nitrogen limitation on polyhydroxyalkanoates production efficiency, properties and microbial dynamics using a soil-derived mixed continuous culture

“…Overall, it could be noted that RISA is a reliable method that can complement other used tools for the identification of the dominant microorganism to the species level. However, stronger molecular tools such as Next Generation Sequencing (NGS) are needed for the complete characterization of the microbial diversity and fingerprinting of complex microbial communities [41,42].…”