New production process of the antifungal chaetoglobosin A using cornstalks

Jiang, Cheng; Jiang, Song; Zhang, Junzheng; Yang, Qian

doi:10.1016/j.bjm.2016.11.008

Cited by 13 publications

(3 citation statements)

References 28 publications

(42 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Chaetoglobosins are a class of mycotoxins with both antifungal and anticancer activities ( ALi et al. 2015 ; Jiang et al. 2017 ) found in the distantly related Chaetomium globosum (Sordariomycetes) and some Eurotiomycetes ( Schümann and Hertweck 2007 ) ( fig.…”

Section: Resultsmentioning

confidence: 99%

The Architecture of Metabolism Maximizes Biosynthetic Diversity in the Largest Class of Fungi

Gluck‐Thaler

Haridas

Binder³

et al. 2020

Molecular Biology and Evolution

View full text Add to dashboard Cite

Abstract Ecological diversity in fungi is largely defined by metabolic traits, including the ability to produce secondary or “specialized” metabolites (SMs) that mediate interactions with other organisms. Fungal SM pathways are frequently encoded in biosynthetic gene clusters (BGCs), which facilitate the identification and characterization of metabolic pathways. Variation in BGC composition reflects the diversity of their SM products. Recent studies have documented surprising diversity of BGC repertoires among isolates of the same fungal species, yet little is known about how this population-level variation is inherited across macroevolutionary timescales. Here, we applied a novel linkage-based algorithm to reveal previously unexplored dimensions of diversity in BGC composition, distribution, and repertoire across 101 species of Dothideomycetes, which are considered the most phylogenetically diverse class of fungi and known to produce many SMs. We predicted both complementary and overlapping sets of clustered genes compared with existing methods and identified novel gene pairs that associate with known secondary metabolite genes. We found that variation among sets of BGCs in individual genomes is due to nonoverlapping BGC combinations and that several BGCs have biased ecological distributions, consistent with niche-specific selection. We observed that total BGC diversity scales linearly with increasing repertoire size, suggesting that secondary metabolites have little structural redundancy in individual fungi. We project that there is substantial unsampled BGC diversity across specific families of Dothideomycetes, which will provide a roadmap for future sampling efforts. Our approach and findings lend new insight into how BGC diversity is generated and maintained across an entire fungal taxonomic class.

show abstract

Section: Resultsmentioning

confidence: 99%

The Architecture of Metabolism Maximizes Biosynthetic Diversity in the Largest Class of Fungi

Gluck‐Thaler

Haridas

Binder³

et al. 2020

Molecular Biology and Evolution

View full text Add to dashboard Cite

show abstract

“…Studies have shown that such core microbiomes have a variety of potential beneficial effects in maintaining soil health and plant growth, such as antibiotic production, heterologous material degradation, heavy metal adsorption, and root colonization ( 35 , 40 , 41 ). Chaetomium produces cellulase and chaetomin, which can inhibit pathogenic fungi growth and promote the activation of soil nutrients ( 42 , 43 ). Penicillium can degrade harmful substances in soil, and its secondary metabolites can significantly inhibit some pathogens ( 44 ).…”

Section: Discussionmentioning

confidence: 99%

Effects of reductive soil disinfestation combined with different types of organic materials on the microbial community and functions

Zhan,

Zhou,

Wang

et al. 2024

Microbiol Spectr

View full text Add to dashboard Cite

Reductive soil disinfestation (RSD) is an effective method to inhibit soilborne pathogens. However, it remains unclear how RSD combined with different types of organic materials affects the soil ecosystems of perennial plants. Pot experiments were conducted to investigate the effects of RSD incorporated with perilla (PF), alfalfa (MS), ethanol, and acetic acid on soil properties, enzyme activities, microbial communities and functions, and seedling growth. Results showed that RSD-related treatments improved soil properties and enzyme activities, changed microbial community composition and structure, enhanced microbial interactions and functions, and facilitated seedling growth. Compared with CK, RSD-related treatments increased soil pH, available nitrogen, and available potassium contents, sucrase and catalase activities, and decreased soil electric conductivity values. Meanwhile, RSD-related treatment also significantly reduced the relative abundance of Fusarium while increasing the relative abundance of Arthrobacter , Terrabacter , and Gemmatimonas . The reduction was more evident in PF and MS treatment, suggesting the potential for RSD combined with solid agricultural wastes to suppress pathogens. Furthermore, the microbial network of RSD-related treatment was more complex and interconnected, and the functions related to carbon, nitrogen, sulfur, and hydrogen cycling were significantly increased, while the functions of bacterial and fungal plant pathogens were decreased. Importantly, RSD-related treatments also significantly promoted seed germination and seedling growth. In summary, RSD combined with solid agricultural wastes is better than liquid easily degradable compounds by regulating the composition and function of microbial communities to improve soil quality and promote plant growth. IMPORTANCE Reductive soil disinfestation (RSD) is an effective agricultural practice. We found that RSD combined with solid agricultural wastes is better than that of liquid easily degradable compounds, may improve soil quality and microbial community structure, inhibit the proliferation of pathogenic bacteria, and contribute to the growth of replanted crops. Thus, RSD combined with solid agricultural wastes is more effective than liquid easily degradable compounds.

show abstract

“…First, C. globosum was grown in 50‐ml PDB and shaken at 28°C for 8 days. Then, the liquid culture was extracted with an equal volume of acetone [21]. Furthermore, the organic phase was concentrated by a vacuum evaporator until dry, under reduced pressure at 55°C.…”

Section: Methodsmentioning

confidence: 99%

Conventional detection and quantification real‐time PCR of the pks‐1 gene of Chaetomium globosum

Gherbawy

El‐Dawy

2020

J Basic Microbiol

View full text Add to dashboard Cite

Chaetomium globosum is known as a potential biocontrol indicator against various soil and seedborne pathogens. Precise data are necessary for population monitoring of C. globosum for its effective use in agriculture. A sequence-characterized amplified region marker has been applied for the detection of this biocontrol agent, which will help to detect C. globosum at the site of its application. Out of 17 isolates of C. globosum, only 8 isolates of C. globosum amplified a monomorphic band of 1,900 bp. C. globosum is known for producing chaetoglobosin A. The pks-1 gene is unique in C. globosum in that it is involved in chaetoglobosin A production, melanin formation, and sporulation. Real-time PCR of pks-1 was used to compare the expressions of the pks-1 gene and chaetoglobosin A biosynthesis and sporulation. It was found that the sporulation of C. globosum was associated with the levels of pks-1 gene expression; Cg2 isolate showed high expression of the pks-1 gene, 41.21%, and also produced a great number of spores and perithecia. The association between the pks-1 gene expression and chaetoglobosin A production was estimated. The Pks-1 gene was expressed by all C. globosum isolates except one isolate, C1, which is another species of Chaetomium. In addition, all C. globosum isolates produced chaetoglobosin A with different concentrations and did not express the same levels of pks-1. This finding may be a result of the solvent type used in the extraction. K E Y W O R D SChaetomium globosum, SCAR marker, pks-1 gene

show abstract

New production process of the antifungal chaetoglobosin A using cornstalks

Cited by 13 publications

References 28 publications

The Architecture of Metabolism Maximizes Biosynthetic Diversity in the Largest Class of Fungi

The Architecture of Metabolism Maximizes Biosynthetic Diversity in the Largest Class of Fungi

Effects of reductive soil disinfestation combined with different types of organic materials on the microbial community and functions

Conventional detection and quantification real‐time PCR of the pks‐1 gene of Chaetomium globosum

Contact Info

Product

Resources

About