Heterologous expression of a rice metallothionein isoform ( OsMTI-1b ) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance

Ansarypour, Zahra; Shahpiri, Azar

doi:10.1016/j.bjm.2016.10.024

Cited by 31 publications

(14 citation statements)

References 44 publications

(63 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The Cys-rich metal-chelating MTs play an important role in metal homeostasis in many organisms [11,[35][36][37][38][39]. Four types of MTs have been characterized in plants [6,14].…”

Section: Discussionmentioning

confidence: 99%

Characterization analysis and heavy metal‐binding properties of CsMTL3 in Escherichia coli

Liu

et al. 2018

FEBS Open Bio

View full text Add to dashboard Cite

Members of the metallothionein ( MT ) superfamily are involved in coordinating transition metal ions. In plants, MT family members are characterized by their arrangement of Cys residues. In this study, one member of the MT superfamily, Cs MTL 3 , was characterized from a complementary DNA ( cDNA ) library from young cucumber fruit; Cs MTL 3 is predicted to encode a 64 amino acid protein with a predicted molecular mass of 6.751 kDa. Phylogenetic analysis identified it as a type 3 family member as the arrangement of N‐terminal Cys residues was different from that of MT‐like 2. Heterologous expression of Cs MTL 3 in Escherichia coli improved their heavy metal tolerance, particularly to Cd 2+ and Cu 2+ , and led to increased uptake of Cd 2+ and Cu 2+ ; increased uptake was also observed for cells expressing Arabidopsis thaliana metallothionein 3 (At MT 3) and phytochelatin‐like ( PCL ), with greatest uptake in PCL ‐expressing cells. These findings demonstrate that Cs MTL 3 can improve metal tolerance, especially for Cd 2+ ions, when heterologously expressed in E. coli , and suggest that the composition and arrangement of N‐terminal Cys residues are associated with binding capacity and preference for different metal ions.

show abstract

“…The Cys-rich metal-chelating MTs play an important role in metal homeostasis in many organisms [11,[35][36][37][38][39]. Four types of MTs have been characterized in plants [6,14].…”

Section: Discussionmentioning

confidence: 99%

Characterization analysis and heavy metal‐binding properties of CsMTL3 in Escherichia coli

Liu

et al. 2018

FEBS Open Bio

View full text Add to dashboard Cite

show abstract

“…Abiotic stresses like HMs induce excessive accumulation of ROS in plants, and cause damages to the cellular macromolecules such as proteins, leading to metabolic and physiological disorders in cells or even cell death (Hasan et al, 2016 ). Interestingly, MTs have been proposed as an alternative tool by which plants protect themselves from stress-induced oxidative damage (Figure 1 ; Hassinen et al, 2011 ; Ansarypour and Shahpiri, 2017 ). Although many reports have indicated the roles of MTs in abiotic stress tolerance as ROS scavengers, the mechanisms through which MTs mediate ROS homeostasis remain unclear (Hassinen et al, 2011 ).…”

Section: Heavy Metals (Hms) Detoxificationmentioning

confidence: 99%

Responses of Plant Proteins to Heavy Metal Stress—A Review

et al. 2017

View full text Add to dashboard Cite

Plants respond to environmental pollutants such as heavy metal(s) by triggering the expression of genes that encode proteins involved in stress response. Toxic metal ions profoundly affect the cellular protein homeostasis by interfering with the folding process and aggregation of nascent or non-native proteins leading to decreased cell viability. However, plants possess a range of ubiquitous cellular surveillance systems that enable them to efficiently detoxify heavy metals toward enhanced tolerance to metal stress. As proteins constitute the major workhorses of living cells, the chelation of metal ions in cytosol with phytochelatins and metallothioneins followed by compartmentalization of metals in the vacuoles as well as the repair of stress-damaged proteins or removal and degradation of proteins that fail to achieve their native conformations are critical for plant tolerance to heavy metal stress. In this review, we provide a broad overview of recent advances in cellular protein research with regards to heavy metal tolerance in plants. We also discuss how plants maintain functional and healthy proteomes for survival under such capricious surroundings.

show abstract

“…In other studies, enhanced tolerance towards ROS or compounds that induce ROS production in non-hypersensitive to oxidative stress yeast cells expressing pMTs was observed (Ansarypour & Shahpiri, 2017;Nishiuchi, Liu, & Takano, 2007;Samardžić et al, 2010;Xue et al, 2009). However, both our and Hegelund et al (2012) results indicated that in yeast mutant strains that are oversensitive to oxidative stress, yap-1Δ and skn7Δ, respectively, expression of pMTs cannot complement or even intensifies the loss of H 2 O 2 tolerance.…”

Section: Potential Of Bnmt1-4 To Scavenge Reactive Oxygen Species Imentioning

confidence: 76%

Potential involvement of rapeseed (Brassica napus L.) metallothioneins in the hydrogen peroxide‐induced regulation of seed vigour

Mierek‐Adamska

Kotowicz

Goc

et al. 2019

J Agronomy Crop Science

View full text Add to dashboard Cite

Germination is a crucial process in crop plant life cycle that determines future yield. Hydrogen peroxide plays a significant role in both dormancy release and seed ageing. The potential of the rapeseed metallothioneins (BnMT1‐4) as reactive oxygen species scavengers was investigated. In the presence of H2O2, bacterial cells expressing BnMT1‐4 had higher growth rate than control cells. However, such effect was not observed in an oxidative stress oversensitive yap‐1Δ yeast mutant strain expressing BnMT1‐4. Next, it was shown that H2O2, up to 100 and 10 mM, promotes rapeseed germination rate and seedling growth, respectively. Moreover, it was demonstrated that in seeds germinating in the presence of H2O2, the expression of seed‐specific BnMT4 decreases to a high extent in a dose‐ and time‐dependent manner. It was shown that the decline in BnMT4 mRNA level, accelerated by up to 10 mM H2O2 treatment, correlates positively with increased rapeseed germination and early growth, underscoring the plausible role of BnMT4 as a H2O2 scavenging protein that level has to be downregulated in conditions where the compound is needed. Finally, it was demonstrated that H2O2 could be used as a priming agent of rapeseeds.

show abstract

Heterologous expression of a rice metallothionein isoform ( OsMTI-1b ) in Saccharomyces cerevisiae enhances cadmium, hydrogen peroxide and ethanol tolerance

Cited by 31 publications

References 44 publications

Characterization analysis and heavy metal‐binding properties of CsMTL3 in Escherichia coli

Characterization analysis and heavy metal‐binding properties of CsMTL3 in Escherichia coli

Responses of Plant Proteins to Heavy Metal Stress—A Review

Potential involvement of rapeseed (Brassica napus L.) metallothioneins in the hydrogen peroxide‐induced regulation of seed vigour

Contact Info

Product

Resources

About