Diarrheagenic Escherichia coli

Gomes, Tânia A. T.; Elias, Waldir P.; Scaletsky, Isabel C. A.; Guth, Beatriz E. C.; Rodrigues, Juliana Falcão; Piazza, Roxane M. F.; Ferreira, Luís Carlos de Souza; Martinez, Marina Baquerizo

doi:10.1016/j.bjm.2016.10.015

Cited by 367 publications

(392 citation statements)

References 417 publications

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“…Diarrhoea is an important cause of mortality and morbidity in different areas of the world (developed and developing countries) and among all age groups [15].The epidemiology of enteric pathogens that cause diarrhoeasuggests that most infections are acquired from food, water,and hand contact and many diarrheal diseases can be prevented by simple rules of personal hygiene and safer foodpreparation [15,16]. In this study, from all stool samples analysed; DEC were detected in 14.7 %of cases, of which 8.5 % were from children under five yearsand 6.2 % from adults.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of <i>Escherichia coli</i> virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Somda¹,

Bonkoungou²,

Zongo³

et al. 2017

Af J Clin Exp Micro

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Objective: Diarrhoeagenic E. coli (DEC) strains are important causes of diarrhoea in the developing world and, to a lesser extent, inthe developed world. In this study, we investigated the prevalence of the virulence genes specific for five major pathogroups of diarrheagenic Escherichia coli (DEC) in primary cultures from diarrhoeagenic patients in Burkina Faso.Methodology: From September 2016 to Mars 2017, a total of 211 faecal samples from diarrhoeagenic patients from urban hospitals of Ouagadou, Burkina Faso have been analysed. A 16-plex PCR was used to detect simultaneously, the five major DEC pathotypes (enteropathogenic E. coli (EPEC), enterotoxigenic E. coli (ETEC), Shiga toxin-producing E. coli (STEC), enteroaggregative E. coli (EAEC) and enteroinvasive E. coli (EIEC)). Results: At least one diarrhoeagenic E. Coli pathotype was detected in 31 samples (14.7%) in children and adults with diarrhoea. EAEC was the most common pathotype detected 9.5% (20/211), followed by EIEC2.4% (05/211) and STEC 0.5% (01/211). More than one DEC pathotype were detected in 2.4% (05/211) patients. EPEC and ETEC were not detected in single infection but in co-infection with others pathotypes. Conclusion: DEC, especially enteroaggregative, may be important responsible of diarrhoeas in Burkina Faso from all ages patient.Key Words: Diarrhoeagenic Escherichia coli, 16-plex PCR, Burkina Faso, human diarrhoeas stool. PREVALENCE DES GENES DE VIRULENCE D'ESCHERICHIA COLIISOLEES DES SELLES DIARRHEIQUES CHEZ LES PATIENTS

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Section: Discussionmentioning

confidence: 99%

Prevalence of <i>Escherichia coli</i> virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Somda¹,

Bonkoungou²,

Zongo³

et al. 2017

Af J Clin Exp Micro

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“…Severe symptoms may even lead to death [1][2][3][4]. Research into EHEC has mainly explored the development of attaching and effacing (A/E) intestinal lesions and the secretion of Shiga toxin in EHEC infection [5][6][7]. The protein EspF is one of the multifunctional effectors of A/E lesions induced by EHEC and enteropathogenic E. coli (EPEC).…”

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confidence: 99%

Screening for host proteins interacting with Escherichia coli O157:H7 EspF using bimolecular fluorescence complementation

Hua

Wang

et al. 2018

Future Microbiology

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Aim:To screen host proteins that interact with enterohemorrhagic Escherichia coli O157:H7 EspF. Materials & methods: Flow cytometry and high-throughput sequencing were used to screen interacting proteins. Molecular function, biological processes and Kyoto Encyclopedia of Genes and Genomes pathways were studied using the DAVID online tool. Glutathione S-transferase pull down and dot blotting were used to verify the interactions. Results: 293 host proteins were identified to associate with EspF. They were mainly enriched in RNA splicing (p = 0.005), ribosome structure (p = 0.012), and involved in 109 types of signaling pathways. SNX9 and ANXA6 were confirmed to interact with EspF. Conclusion: EspF interacts with ANXA6; they may form a complex to manipulate the process of phagocytosis; EspF plays a highlighted pathogenic role in enterohemorrhagic E. coli infection process. Enterohemorrhagic Escherichia coli (EHEC) O157:H7 is an important food-borne causative agent in sporadic outbreaks of illness such as diarrhea, hemorrhagic colitis and hemolytic-uremic syndrome. Severe symptoms may even lead to death [1][2][3][4]. Research into EHEC has mainly explored the development of attaching and effacing (A/E) intestinal lesions and the secretion of Shiga toxin in EHEC infection [5][6][7]. The protein EspF is one of the multifunctional effectors of A/E lesions induced by EHEC and enteropathogenic E. coli (EPEC). EspF participates in a number of damage processes in host cells, targeting mitochondria and nucleoli [6,8] and disrupting the tight junctions (TJs) of intestinal epithelial cells [9], leading to cytoskeletal rearrangements, actin polymerization and pedestal formation [10]. EspF thus emerges as the 'Swiss army knife' of a bacterial pathogen [11,12]. EHEC O157:H7 employs a type-III secretion system to export toxic factors and effector proteins to host cells after adhering to the brush border of epithelial cells [5,[13][14]. The production of virulence factors, subsequent invasion and diffusion, and the interaction of effector proteins with host proteins are key steps in EHEC O157:H7 pathogenesis. The mechanism by which EspF breaks through the intestinal epithelial barrier into host cells, the host proteins that EspF interacts with, and how cellular damage, and even apoptosis, result remain unclear. Studying the pathogen-host interaction process will increase the emerging knowledge about EHEC O157:H7 pathogenesis.The EspF protein has been shown to induce apoptosis after 'injection' into host cells [1]. The N-terminal (1-73 amino acids [aa]) of this approximately 27 kDa protein contains a secretory signal (1-20 aa), a mitochondrialtargeting signal (1-24 aa) and a nucleolar-targeting domain (21-74 aa); 73-248 aa consists of four proline-rich repeats (PRRs), each containing a eukaryotic cell sorting nexin 9 (SNX9) protein-binding site SH3 motif, an

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“…In order to identify different groups of E. coli, their associated specific primers were utilized, including bfp and eaeA for EPEC; lt and st for ETEC; ipaH and virF for EIEC; stx1, stx2, and eaeA for EHEC; and agg and aap for EAEC ( E. coli strains that were positive for aggR and/or aap genes were interpreted as being EAEC and those positive for stx1 and stx2, as STEC. The EIEC were those positive for virF and/or ipaH [7,8].…”

Section: Pcr To Detect Diarrheagenic E Coli Virulence Genesmentioning

confidence: 99%

Characterization of diarrheagenic E. coli causing a diarrheal outbreak in the south of Iran, Summer 2015

Pouladfar¹,

Arastehfar²,

Aminshahidi³

et al. 2017

APJTD

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Diarrheagenic Escherichia coli

Cited by 367 publications

References 417 publications

Prevalence of <i>Escherichia coli</i> virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Prevalence of <i>Escherichia coli</i> virulence genes in patients with diarrhoea in Ouagadougou, Burkina Faso

Screening for host proteins interacting with Escherichia coli O157:H7 EspF using bimolecular fluorescence complementation

Characterization of diarrheagenic E. coli causing a diarrheal outbreak in the south of Iran, Summer 2015

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