The effect of heavy metal contamination on the bacterial community structure at Jiaozhou Bay, China

Abstract

In this study, determination of heavy metal parameters and microbiological characterization of marine sediments obtained from two heavily polluted sites and one low-grade contaminated reference station at Jiaozhou Bay in China were carried out. The microbial communities found in the sampled marine sediments were studied using PCR-DGGE (denaturing gradient gel electrophoresis) fingerprinting profiles in combination with multivariate analysis. Clustering analysis of DGGE and matrix of heavy metals displayed similar occurrence patterns. On this basis, 17 samples were classified into two clusters depending on the presence or absence of the high level contamination. Moreover, the cluster of highly contaminated samples was further classified into two sub-groups based on the stations of their origin. These results showed that the composition of the bacterial community is strongly influenced by heavy metal variables present in the sediments found in the Jiaozhou Bay. This study also suggested that metagenomic techniques such as PCR-DGGE fingerprinting in combination with multivariate analysis is an efficient method to examine the effect of metal contamination on the bacterial community structure.

Next-generation sequencing showing potential leachate influence on bacterial communities around a landfill in China

“…pH was also shown to significantly influence the bacterial community of leachate. The findings from this paper are consistent with previous results that show that heavy metals influence the bacterial community of landfill (Yao et al, 2017).…”

“…Previous studies on heavy metal influence towards microbial communities were performed using PCR-DGGE and GS 454 FLX pyrosequencing (Muller et al, 2001, Yao et al, 2017, Vishnivetskaya et al, 2011. Next generation sequencing (NGS) methods can assist in the identification of very rare taxa in the landfill samples (Köchling et al, 2015, Song et al, 2015a.…”

“…Previous studies have indicated a diverse range of heavy metal concentrations in leachates (Song et al, 2015b, Zhang et al, 2013a. Heavy metals have been previously shown to directly influence the bacterial community composition of various environments (Muller et al, 2001, Vishnivetskaya et al, 2011, Sandaa et al, 1999, Mor et al, 2006, Yao et al, 2017. Long term studies have shown a strong influence of mercury towards the bacterial community of a river basin and soil (Muller et al, 2001).…”

Aquatic microbial diversity associated with faecal pollution of Norwegian waterbodies characterized by 16S rRNA gene amplicon deep sequencing

“…This can be related to various stress factors accompanying faecal water contamination that affect the basic ecological processes of ecosystems, leading to suppression of some microbes and enrichment of others, disruption in natural processes of biodegradation, water flow and nutrient availabilities. A negative response of the aquatic microbial community to the combined impacts of organic and inorganic pollutants, and variations in temperature, oxygen, salinity, acidification, nutrient and organic matter variabilities have been widely observed (Van Horn et al , ; Pratt et al , ; Mahmoudi et al , ; Ibekwe et al , ; Mesa et al , ; Yao et al , ).…”

Kapasitas Hidrolisis Bakteri Pendegradasi Selulosa Dari Ekosistem Mangrove

“…Komunitas bakteri selulolitik diketiga kategori mangrove dimungkinkan memiliki perbedaan dengan keberadaan logam berat yang berpotensi menjadi penyebab seleksi bakteri. Yao et al, (2017)…”