Selection, isolation, and identification of fungi for bioherbicide production

Souza, Angélica Rossana Castro de; Baldoni, Daiana Bortoluzzi; Lima, Jessica; Porto, Vitória; Marcuz, Camila; Machado, Carolina; Ferraz, Rafael Camargo; Kuhn, Raquel C.; Jacques, Rodrigo Josemar Seminoti; Guedes, Jerson Vanderlei Carús; Mazutti, Márcio A.

doi:10.1016/j.bjm.2016.09.004

Cited by 56 publications

(32 citation statements)

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“…Several groups have recently isolated and identified Diaporthe species that have potential for use as microbial bioherbicides. [31][32][33] Bastos et al 34 have shown enhanced efficacy of herbicidal effect of the fermented broths of Diaporthe species by formulation with wileyonlinelibrary.com/journal/ps adjuvants. None of these studies have related the activity of these microbes to the production of specific phytotoxins.…”

Section: N-(tert-butyl)-2-chloro-6-(2-hydroxy-2-methylpropyl)-3-methyl-mentioning

confidence: 99%

Isolation of a phytotoxic isocoumarin from Diaporthe eres‐infected Hedera helix (English ivy) and synthesis of its phytotoxic analogs

Meepagala

Briscoe

Techen³

et al. 2017

Pest Management Science

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BACKGROUND: The fungus Diaporthe eres was isolated from a fungal pathogen-infected leaf of Hedera helix (English ivy) exhibiting necrosis. It is hypothesized that the causative fungus produces phytotoxins as evidenced by necrotic lesions on the leaves. RESULTS:The fungus was isolated and grown in Czapek Dox broth culture medium and potato dextrose broth culture medium and identified as Diaporthe eres. The ethyl acetate extracts of the culture broths were phytotoxic to lettuce (Lactuca sativa) and bentgrass (Agrostis stolonifera). 3,4-Dihydro-8-hydroxy-3,5-dimethylisocoumarin (1) and tyrosol (2) were isolated and identified as the phytotoxic constituents. Six analogs of 3,4-dihydro-isocoumarin were synthesized and shown to be phytotoxic. The synthesized 3,4-dihydro-8-hydroxy-3,7-dimethylisocoumarin and 3,4-dihydro-8-hydroxy-3,3,7-trimethylisocoumarin were two-to three-fold more phytotoxic than the naturally occurring 1 in a Lemna paucicostata growth bioassay.CONCLUSION: Synthesis and herbicidal activities of the several new analogs of 1 are reported for the first time. These promising molecules should be used as templates for synthesis and testing of more analogs.

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Section: N-(tert-butyl)-2-chloro-6-(2-hydroxy-2-methylpropyl)-3-methyl-mentioning

confidence: 99%

Isolation of a phytotoxic isocoumarin from Diaporthe eres‐infected Hedera helix (English ivy) and synthesis of its phytotoxic analogs

Meepagala

Briscoe

Techen³

et al. 2017

Pest Management Science

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“…However, these compounds have the potential to provide a competitive advantage for seedling growth through infection and growth retardation of weed seedlings. Therefore, isolation and structural characterization and mode of action of phytotoxins produced by pathogenic fungi for weeds, including aquatic invasive plants, should be investigated [41].…”

Section: Resultsmentioning

confidence: 99%

Biological Potencial of Colletotrichum typhae H.C Greene mycoherbicide for Typha domingensis Pers

Maia

Melo

Barreto

et al. 2018

Preprint

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24 The anthropic interference in aquatic ecosystems, favors the disordered colonization of T. domingensis, 25 damaging the production of hydroelectric power and river traffic. Thus, the objective of this study was 26 to evaluate the potential of C. typhae as a mycoherbicide in the control of T. domingensis, in vitro and 27 in greenhouse. 107 samples of symptomatic T. domingensis leaves were collected in flooded areas of 28 rivers in Brazil, with identification and isolation of the collected fungal species. The concentration of 29 inoculum was determined to evaluate the incidence and severity of the disease, the influence of 30 temperature on mycelial growth and conidia germination, the effect of temperature and leaf wetness 31 period on T. domingensis infection by C. typhae and the host range test. The growth of the colonies of 32 C. typhae was higher at 25 to 30 ºC, there was no interference of the photoperiod on germination of the 33 spores, but the highest percentage of germination occurred at 17.39 ºC. The influence of environmental 2 34 conditions on infection of inoculated leaves of T. dominguensis indicated that at 15 ºC and the period of 35 leaf wetness of 48 hours promoted the highest incidence of the disease, as well as the severity for the 36 same period of leaf wetness. The specificity test showed that C. typhae is specific and pathogenic to T. 37 domingensis. Being this the first report of the occurrence of this pathogen in aquatic macrophytes of 38 this species and in T. domingensis in Brazil. 39 Keywords: Biological control; Aquatic macrophytes, Leaf wetness 40 41 Introduction 42 Typha domingensis Pers. is an invasive macrophyte found in the Americas, Europe, Africa, Asia and 43 Oceania, being considered as a native species of South America, occurring throughout Brazil [1]. It is 44 propagated either by seeds or vegetatively, by rhizomes, with vigorous growth by the decomposition 45 and assimilation of organic matter as a source of nutrients, reaching about seven tons of rhizomes per 46 hectare [2]. Thus, T. domingensis is used as a biological filter for urban sewage, industrial effluents rich 47 in heavy metals and erosion control in drainage channels and reservoir banks [3]. 48However, anthropic interference in aquatic ecosystems favors the colonization of T. domingensis, 49 which may hinder the production of hydroelectric power, river traffic and agricultural irrigation [4]. In 50 the United States, this macrophyte accounts for the degradation of almost 12.000 ha -1 of Florida 51 marshes, due its aggressive growth in response to eutrophication by nitrates and phosphates from 52 agricultural and urban waste and frequent fires [5]. In Brazil, it is estimated that the intense growth of 53 T. domingensis in reservoirs of the country's hydroelectric dams extends to about 300 ha -1 in the water 54 mirror. These changes contribute to the reduction of water quality and biodiversity patterns in 55 environments colonized by this species [6]. 56Up to a certain limit, the development of aquatic vegetation c...

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“…The strain used in this study was Diaporthe sp., previously isolated by Souza et al (2016). The culture was maintained in a potato dextrose agar (PDA) at 4°C and subcultured every 15 days.…”

Section: Microorganism and Inoculummentioning

confidence: 99%

“…has shown interesting results (Souza et al 2015(Souza et al , 2016Pes et al 2016;Briscoe 2014). Species of Diaporthe has been studied for production of molecules with antibacterial (Specian et al 2012), antifungal (Prada et al 2009) and for control of Phyllosticta citricarpa in citrus (Santos et al 2016).…”

Section: Introductionmentioning

confidence: 97%

Solid-state fermentation for production of a bioherbicide from Diaporthe sp. and its formulation to enhance the efficacy

et al. 2017

Self Cite

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In this study, a bioherbicide was produced by solid-state fermentation (SSF) using Diaporthe sp. Adjuvants were employed in a formulation to enhance the herbicidal activity towards the target (Cucumis sativus). The study was divided into two steps: (1) the fermentation condition for bioherbicide production was assessed; (2) evaluation of different formulations containing palm oil, Tween Ò 80 and Span Ò 80, in order to increase phytotoxicity. In step 1, the maximum herbicidal activity (1.23% of the leaves had lesions) was obtained at 25°C, moisture content of 50 wt%, supplemented with 10 wt% of corn steep liquor and soybean bran and inoculum density of 15 wt%. In step 2, the formulation containing 8.2 wt% of palm oil, 8.2 wt% of Tween Ò 80 and Span Ò 80, resulting in an HLB of 12.8 showed the highest phytotoxicity on the leaves. At this condition, dry matter and height of target were reduced about 36% in comparison with control. Diaporthe sp. has the potential to produce molecules with herbicidal activity and the use of adjuvants enhanced three times its efficiency.

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Selection, isolation, and identification of fungi for bioherbicide production

Cited by 56 publications

References 35 publications

Isolation of a phytotoxic isocoumarin from Diaporthe eres‐infected Hedera helix (English ivy) and synthesis of its phytotoxic analogs

Isolation of a phytotoxic isocoumarin from Diaporthe eres‐infected Hedera helix (English ivy) and synthesis of its phytotoxic analogs

Biological Potencial of Colletotrichum typhae H.C Greene mycoherbicide for Typha domingensis Pers

Solid-state fermentation for production of a bioherbicide from Diaporthe sp. and its formulation to enhance the efficacy

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