Genome sequence of Prevotella intermedia SUNY aB G8-9K-3, a biofilm forming strain with drug-resistance

Moon, Ji-Hoi; Kim, Minjung; Lee, Jae Hyung

doi:10.1016/j.bjm.2016.04.030

Cited by 2 publications

(3 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…1 Prevotella intermedia mampu membentuk biofilm dan memiliki exopolysaccharides yang terdiri dari gula netral dan manosa dan merupakan komponen utama pembentukan biofilm dan memiliki kemampuan untuk menghindari sistem kekebalan manusia. 2 Prevotella intermedia dikenal sebagai bakteri periodontopatik yang aktivitas metaboliknya sering mengakibatkan percepatan perkembangan penyakit yang dimediasi biofilm oral. 3 Metode kolonisasinya dengan adhesi antar permukaan bakteri, protein pengikat pada permukaan sel yang memiliki kemampuan adhesi mikroba ditemukan pada Prevotella intermedia.…”

Section: Pendahuluanunclassified

“…10 Beberapa tanaman telah diteliti melalui aktivitas antibakteri terhadap beberapa bakteri patogen pada rongga mulut. 2 Terdapat komponen bioaktif pada tanaman yang memiliki efek anti bakteri. Penelitian yang dilakukan di Kampung Pabuaran (Desa Cibanteng), dan Kampung Gunung Leutik (Desa Benteng) terdapat beberapa spesies tanaman yang sering digunakan sebagai obat dalam mengatasi infeksi salah satunya adalah jeruk nipis.…”

Section: Pendahuluanunclassified

“…Pembuatan suspensi bakteri menggunakan standar Brown III dengan mengambil 4-5 ose bakteri dan dilarutkan dalam 10ml NaCL. 2 Pembuatan standar kekeruhan dengan larutan McFarland 0,5 dengan larutan H2SO4 1% sebanyak 9,95ml, dicampur dengan larutan BaCl2 1,175% sebanyak 0,05ml dalam tabung reaksi. Dikocok sampai larutan menjadi keruh, kekeruhan digunakan sebagai standar kekeruhan suspensi bakteri uji.…”

Section: Persiapan Bakteri Prevotella Intermediaunclassified

See 2 more Smart Citations

Aktivitas antibakteri ekstrak kulit jeruk nipis (Citrus aurantifolia) terhadap bakteri Prevotella intermediaAntibacterial activity of lime (Citrus aurantifolia) peel extract towards Prevotella intermedia

2021

View full text Add to dashboard Cite

Pendahuluan: Prevotella intermedia merupakan salah satu bakteri utama pada penyakit periimplantitis. Periimplantitis merupakan inflamasi jaringan lunak dan keras disekitar implan yang dapat dicegah menggunakan ekstrak tanaman antibakteri. Salah satunya yaitu kulit jeruk nipis, yang dapat menghambat proses inflamasi karena mengandung alkaloid, steroid, saponin, flavonoid, tanin sebagai senyawa antibakteri. Penelitian ini bertujuan untuk mengetahui peranan antibakteri kulit jeruk nipis dengan mengukur Konsentrasi Hambat Minimal (KHM) dan Konsentrasi Bunuh Minimal (KBM) ekstrak kulit jeruk nipis terhadap pertumbuhan bakteri Prevotella intermedia. Metode: Eksperimental laboratorium dengan rancangan penelitian post test only control group design. Pengujian KHM dan KBM dilakukan dengan metode dilusi, kulit jeruk nipis dimaserasi menggunakan pelarut etanol 70% sehingga didapatkan ekstrak kulit jeruk nipis dengan konsentrasi 0,78, 1,56, 3,125, 6,25, 12,5, 25, 50, dan 100% dengan chlorhexidine sebagai kontrol positif dan akuades sebagai kontrol negatif. Media kultur bakteri menggunakan Tripton Soya Agar. Hasil: Berdasarkan analisis statistik menggunakan Kruskal Wallis menunjukkan perbedaan penurunan jumlah koloni bakteri yang signifikan (p=0,0001) pada KBM dan KHM dari berbagai konsentrasi ekstrak kulit jeruk nipis terhadap pertumbuhan bakteri Prevotella intermedia, uji lanjutan Mann Whitney menunjukkan perbedaan penurunan jumlah koloni bakteri yang signifikan (p=0,021) antar masing-masing konsentrasi dan kelompok kontrol. Simpulan: Konsentrasi hambat minimal ekstrak kulit jeruk nipis terhadap bakteri Prevotella intermedia 12,5%, konsentrasi bunuh minimalnya 25%. Kata kunci: agen antibakteri; ekstrak jeruk nipis; Prevotella intermediaABSTRACTIntroduction: Prevotella intermedia is one of the main bacteria in periimplantitis. Periimplantitis is inflammation of the soft and hard tissues around the implant that can be prevented using antibacterial plant extracts. One of them is lime peel, which can inhibit the inflammatory process due to its alkaloids, steroids, saponins, flavonoids, and tannins as antibacterial compounds. This study was aimed to determine the antibacterial activity of lime peel by measuring the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of lime peel extract on the growth of Prevotella intermedia. Methods: Experimental laboratory with post-test only control group design. The MIC and MBC tests were performed by the dilution method. The lime peel was macerated using 70% ethanol solvent to obtain lime peel extract with concentrations of 0.78, 1.56, 3.125, 6.25, 12.5, 25, 50, and 100% with chlorhexidine as a positive control and aquadest as a negative control. Bacterial culture media using Tripton Soy Agar. Results: Based on statistical analysis using Kruskal Wallis showed a significant difference in the decrease of the number of bacterial colonies (p=0.0001) in MBC and MIC from various concentrations of lime peel extract on the growth of Prevotella intermedia bacteria. Furthermore, the Mann Whitney follow-up test showed differences in the decrease of the number of bacterial colonies, which was significant (p=0.021) between each concentration and control group. Conclusions: The minimum inhibitory concentration of lime peel extract towards the growth of Prevotella intermedia was 12.5%, with the minimum bactericidal concentration of 25%. Keywords: antibacterial agent; citrus extracts; Prevotella intermedia

show abstract

Section: Pendahuluanunclassified

Section: Persiapan Bakteri Prevotella Intermediaunclassified

See 1 more Smart Citation

Aktivitas antibakteri ekstrak kulit jeruk nipis (Citrus aurantifolia) terhadap bakteri Prevotella intermediaAntibacterial activity of lime (Citrus aurantifolia) peel extract towards Prevotella intermedia

2021

View full text Add to dashboard Cite

show abstract

Effects of urbanization and lifestyle habits on the intestinal microbiota of adolescents in eastern China

Zhao

Xie

et al. 2023

Front. Microbiol.

View full text Add to dashboard Cite

IntroductionOwing to urbanization, living habits have changed widely, leading to alterations in the intestinal microbiota of urban residents. However, there are few studies on the characteristics of intestinal microbiota of adolescents living in different urbanized areas in China.MethodsA total of 302 fecal samples collected from adolescent students in eastern China were examined. 16S rRNA high-throughput sequencing was used to identify the fecal microbiota. These data were combined with questionnaire survey results to investigate the effect of urbanization on the intestinal microbiota of adolescents in eastern China. Moreover, the role of lifestyle habits in this relationship was also evaluated.ResultsThe results revealed significant differences in the structure of the intestinal microbiota among adolescents living in regions with different levels of urbanization. Adolescents living in urban regions had a significantly higher proportion of Bacteroides (p < 0.001, FDR = 0.004), whereas those living in towns and rural regions had higher proportions of Bifidobacterium (p < 0.001, FDR < 0.001) and Prevotella (p < 0.05, FDR = 0.019). The diversity of the intestinal microbiota was higher in urban residents than in adolescents living in towns and rural regions (p < 0.05). In addition, the differences in intestinal microbiota across individuals living in cities, towns, and rural regions were related to dietary preferences, flavor preferences, and sleep and exercise durations. Adolescents who ate more meat had more Dorea (LDA = 3.622, p = 0.04), while the abundance of Escherichia–Shigella is higher among adolescents who ate more condiments (LDA = 4.285, p = 0.02). The abundance of Dialister was significantly increased in adolescents with longer sleep durations (LDA = 4.066, p = 0.03). Adolescents who exercised for a long duration had more Faecalibacterium than those who exercised for a shorter duration (LDA = 4.303, p = 0.04).DiscussionOur research has preliminarily demonstrated that there were differences in the composition of Gut microbiome in stool samples of adolescents living in different urbanized areas, and provide a scientific basis for the maintenance of a healthy intentional microbota in adolescences.

show abstract

Genome sequence of Prevotella intermedia SUNY aB G8-9K-3, a biofilm forming strain with drug-resistance

Cited by 2 publications

References 7 publications

<p>Aktivitas antibakteri ekstrak kulit jeruk nipis (Citrus aurantifolia) terhadap bakteri Prevotella intermedia</p><p>Antibacterial activity of lime (Citrus aurantifolia) peel extract towards Prevotella intermedia <p>

<p>Aktivitas antibakteri ekstrak kulit jeruk nipis (Citrus aurantifolia) terhadap bakteri Prevotella intermedia</p><p>Antibacterial activity of lime (Citrus aurantifolia) peel extract towards Prevotella intermedia <p>

Effects of urbanization and lifestyle habits on the intestinal microbiota of adolescents in eastern China

Contact Info

Product

Resources

About