Ligninolytic fungus Polyporus sp. S133 mediated metabolic degradation of fluorene

Abstract

This study aimed to investigate the impact of nonionic surfactants on the efficacy of fluorine degradation by Polyporus sp. S133 in a liquid culture. Fluorene was observed to be degraded in its entirety by Polyporus sp. S133 subsequent to a 23-day incubation period. The fastest cell growth rate was observed in the initial 7 days in the culture that was supplemented with Tween 80. The degradation process was primarily modulated by the activity of two ligninolytic enzymes, laccase and MnP. The highest laccase activity was stimulated by the addition of Tween 80 (2443 U/L) followed by mixed surfactant (1766 U/L) and Brij 35 (1655 U/L). UV–vis spectroscopy, TLC analysis and mass spectrum analysis of samples subsequent to the degradation process in the culture medium confirmed the biotransformation of fluorene. Two metabolites, 9-fluorenol (λmax 270, tR 8.0 min and m/z 254) and protocatechuic acid (λmax 260, tR 11.3 min and m/z 370), were identified in the treated medium.

Enhancement of eritadenine production using three carbon sources, immobilization and surfactants in submerged culture with shiitake mushroom (Lentinula edodes) (Berk.) Singer)

“…Similarly to others, surfactants have specific effects on fungal metabolism (Jakovljevi et al, 2014;Lazim et al, 2016). In this study, surfactant Tween 20 enhanced significantly eritadenine yield, as shown in Table 1.…”

“…(Hadibarata and Kristanti, 2013). Probably, surfactants enable mycelial cells to absorb nutrients at a faster rate promoting better cell multiplication (Lazim et al, 2016), which can be happening here for shiitake. The present results with tweens found an interesting tendency: Tween 20 promotes more metabolites, but not the growth, whilst tween 80 promotes good growth, but few metabolite production.…”

Surfactant‐aided mycoremediation of soil contaminated with polycyclic aromatic hydrocarbon (PAHs): progress, limitation, and countermeasures

“…In this aspect, the addition of a surfactant was reported to have a positive effect in making the hydrophobic cellulose more easily available for the enzymatic hydrolysis. 38 In another study, the presence of soybean oil was found to enhance the growth of fungi -P. ostreatus, 126 Grifola frondosa, 127 T. hirsuta, 128 Polyporus sp 129 during soil remediation in the presence of PAHs. On the other hand, in the real soil matrix, a variety of microorganisms were reported to have the ability to metabolize the surfactant as a carbon source, which often leads to unnecessary nutrient competition to the bioaugmented fungi.…”

Development of activated carbon from Eichhornia Crassipes via chemical activation and its application to remove a synthetic dye

“…The conventional wastewater treatment includes biological, chemical and physical treatment processes. Various conventional treatment was proposed to eliminate dyes from environment such as filtration, sedimentation, aerobic and anaerobic techniques, oxidation, and other physical and chemical process [6][7][8][9][10][11][12][13]. Although, those conventional methods showed their ability for eliminating dye at small scale, it can't be implemented in big scale or industrial scale due to high cost and energy utilization, generate a huge amount of by-product, and not ecofriendly.…”