Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12

Portal, Thayara Morais; Siqueira, Jones Anderson Monteiro; Costa, Larissa Kelly Cunha; Lima, Ian Carlos Gomes de; Lucena, Maria Silvia Sousa de; Bandeira, Renato da Silva; Linhares, Alexandre da Costa; Luz, Cláudia Regina Nunes Eloi da; Gabbay, Yvone Benchimol; Resque, Hugo Reis

doi:10.1016/j.bjm.2016.04.008

Cited by 13 publications

(3 citation statements)

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“…However, a research conducted by Siqueira et al [ 51 ] demonstrated a good performance using the same EIA kit, with a sensitivity of 92% and a specificity of 83.3%. This methodology has already been used in other studies, carried out in the Amazon region, establishing higher positive rates [ 44 , 52 , 53 ]. Uncommon recombinants strains (GII.P21/GII.2, GII.13/GII.17, GII.P21/GII.3) and emergent variants (GII.4_Sydney) were also detected by EIA [ 50 , 54 ].…”

Section: Discussionmentioning

confidence: 99%

Molecular epidemiology and temporal evolution of norovirus associated with acute gastroenteritis in Amazonas state, Brazil

et al. 2018

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BackgroundGlobally, Norovirus (NoV) is considered the most common cause of diarrheal episodes across all age groups. Despite its wide genetic diversity, the GII.4 strain is the most predominant and has been associated with epidemics worldwide. In this study, we characterized sporadic cases of diarrhea from NoV-positive children, during a five-year period (2010–2014).MethodsA total of 250 NoV-positive samples identified by an enzyme immunoassay (EIA) were subjected to RT-PCR and partial nucleotide sequencing for polymerase and capsid genes. Phylogenetic analysis was performed to identify NoV genotypes using the binary classification. In addition, sequences from the P2 subdomain (capsid) gene of GII-4 variants were characterized by evolutionary analyses, using the MCMC method implemented in the BEAST package. A 3D structure was built using protein modeling.ResultsPhylogenetic analysis demonstrated a predominance of genotype GII.4 (52.4% - 99/189), variants New Orleans_2009 and Sydney_2012 followed by GII.P7/GII.6 with 6.3% (12/189). Amino acid analyses of the GII.4 strains showed several important amino acid changes. A higher evolutionary rate was found, 7.7 × 10− 3 in the Sydney variant and 6.3 × 10− 3 in the New Orleans. Based in evolutionary analysis the time to the most recent common ancestor (TMRCA) has been calculated as estimates of the population divergence time. Thus, TMRCA for New Orleans and Sydney variant were 2008.7 and 2010.7, respectively. Also, we observed a lineage of transition between New Orleans and Sydney.ConclusionThis study describes the different strains of norovirus isolated from Amazonas state in Brazil during a five-year period. Considering that NoV are capable of changing their antigenic epitopes rapidly, a continuous surveillance is important to monitor the occurrence and changes of the NoV in the community through epidemiological studies. These results contribute to the understanding of NoV molecular epidemiology and its evolutionary dynamics in Amazonas state, Brazil.Electronic supplementary materialThe online version of this article (10.1186/s12879-018-3068-y) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Molecular epidemiology and temporal evolution of norovirus associated with acute gastroenteritis in Amazonas state, Brazil

et al. 2018

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“…The nonenveloped Norovirus (NV; Caliciviridae) is composed of a single strand of RNA (27–40 nanometers diameter) [ 1 ]. NV can be divided into seven genotypes (GI, GII, GIII, GIV, GV, GVI, and GVII) based on complete amino acid sequence analysis for the capsid protein; each genotype can be divided into several subtypes [ 2 , 3 ].…”

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confidence: 99%

Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip

Zhang

Peng

Jiang

et al. 2018

BioMed Research International

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This study was designed to prepare and evaluate the sensitivity and specificity of a Norovirus GI and GII fluorescent particles combined detection test strip method. Using selected chromatographic materials and antibodies specific to Norovirus GI and GII, the Norovirus GI and GII fluorescent particles combined detection test strip (tested method) was prepared as a conventional double antibody sandwich. The samples assayed included cultured rotavirus and 465 specimens from patients with symptoms of gastrointestinal infection. Norovirus was detected using the tested method and a reference method (CerTest Norovirus GI-GII test card). The results indicated that the sensitivity of the tested method was 4 (for GI detection) or 8 times (for GII detection) greater than the reference method. Neither of the two methods cross-reacted with rotavirus and so on. For specimens, 29 were found to be negative by the reference method and positive by the tested method, and 8 were found to be negative by the tested method and positive by the reference method. Furthermore, a retesting of these samples by qPCR showed that 28 of the 29 were positive, and 3 of the 8 were positive. In summary, the Norovirus GI and GII fluorescent particles combined detection test strip was successfully prepared and had good detection performance.

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“…In Brazil, the most common genotypes identified are GI.1, GI.2 and GII.1. 5 , 6 , 7 , 8 Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Phylogenetic analysis was performed for comparison with other previously reported genogroups/genotypes.…”

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Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil

Cilli

Luchs

Leal

et al. 2019

Mem. Inst. Oswaldo Cruz

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Human sapoviruses (HSaV) are considered important causative agents of acute gastroenteritis in humans worldwide. However, knowledge of the genetic characteristics of the whole genome of HSaV in Brazil is limited. Here we report the complete genome sequences of six HSaVs GI.2 and two GI.3 strains obtained from children with acute gastroenteritis in the Northern region of Brazil. Next generation sequencing was used to obtain the full genome and molecular characterization of the genome was performed. Phylogenetic analysis of the genome was also performed. Only one complete HSaV GI.2 genome characterization in the country precedes that of the present study. This is the first complete genome sequence of genotype GI.3 in Brazil. The data obtained in this investigation can contribute to the augmentation of the database on the molecular diversity of HSaVs strains circulating in Brazil, and to the improvement of current typing protocols.

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Caliciviruses in hospitalized children, São Luís, Maranhão, 1997–1999: detection of norovirus GII.12

Cited by 13 publications

References 41 publications

Molecular epidemiology and temporal evolution of norovirus associated with acute gastroenteritis in Amazonas state, Brazil

Molecular epidemiology and temporal evolution of norovirus associated with acute gastroenteritis in Amazonas state, Brazil

Preparation and Evaluation of Combined Detection of Norovirus GI and GII: An Innovative Fluorescent Particles Test Strip

Human sapovirus GI.2 and GI.3 from children with acute gastroenteritis in northern Brazil

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