Curli fimbria: an Escherichia coli adhesin associated with human cystitis

Cordeiro, Melina Aparecida; Werle, Catierine Hirsch; Milanez, Guilherme Paier; Yano, Tomomasa

doi:10.1016/j.bjm.2016.01.024

Cited by 34 publications

(28 citation statements)

References 21 publications

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“…The presence of ompA in several E. coli pathotypes has been reported [41]; therefore, a PCR assay for the detection of this gene was performed in a collection of E. coli strains isolated from different sources, and a prevalence of 55% was observed. Similar studies also reported a prevalence of 93.3% in several pathotypes [42]; in Egypt, E. coli strains associated with UTIs had a prevalence of 17.2% [43]. These results show that OmpA is not an exclusive factor of a particular E. coli pathotype.…”

Section: Discussionsupporting

confidence: 75%

Effect and Analysis of Bacterial Lysates for the Treatment of Recurrent Urinary Tract Infections in Adults

et al. 2020

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Urinary tract infection (UTI) is a relevant public health problem, economically and socially affecting the lives of patients. The increase of antimicrobial bacterial resistance significantly hinders the treatment of UTIs, raising the need to search for alternative therapies. Bacterial lysates (BL) obtained from Escherichia coli and other pathogens have been used to treat different infectious diseases with promising results. This work aims to evaluate the effect and composition of an autologous BL for the treatment and control of recurrent UTIs in adults. The results show remission in 70% of the patients within the first three months after the administration of BL, while the infection is maintained under control for 6–12 months. The analysis by liquid chromatography–mass spectrometry (LC-MS) of the BL fractions recognized by the sera of patients shows the presence of cytosolic proteins, fimbriae, OMPs, and LPS. Our study demonstrates that the autologous BL contributed to the treatment and control of recurrent UTIs in adults, and its composition shows that different surface components of E. coli are potential immunogens that could be used to create a polyvalent protective vaccine.

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Section: Discussionsupporting

confidence: 75%

Effect and Analysis of Bacterial Lysates for the Treatment of Recurrent Urinary Tract Infections in Adults

et al. 2020

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“…Consistent with this study, Humphries and colleagues observed seroconversion to various fimbrial antigens, including CsgA, following infection with S. Typhimurium (58). In a study of 31 clinical isolates of uropathogenic E. coli (UPEC) from patients with reoccurring urinary tract infections (UTIs), all clinically isolated strains were positive for csgA expression (59). The authors determined that curli is an integral factor involved in cell adhesion during UTI and during UPEC-induced cystitis (59).…”

Section: Amyloid Curlimentioning

confidence: 72%

“…In a study of 31 clinical isolates of uropathogenic E. coli (UPEC) from patients with reoccurring urinary tract infections (UTIs), all clinically isolated strains were positive for csgA expression (59). The authors determined that curli is an integral factor involved in cell adhesion during UTI and during UPEC-induced cystitis (59).…”

Section: Amyloid Curlimentioning

confidence: 99%

Curli-Containing Enteric Biofilms Inside and Out: Matrix Composition, Immune Recognition, and Disease Implications

Tursi

Tükel

2018

Microbiol Mol Biol Rev

111

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SUMMARYBiofilms of enteric bacteria are highly complex, with multiple components that interact to fortify the biofilm matrix. Within biofilms of enteric bacteria such asEscherichia coliandSalmonellaspecies, the main component of the biofilm is amyloid curli. Other constituents include cellulose, extracellular DNA, O antigen, and various surface proteins, including BapA. Only recently, the roles of these components in the formation of the enteric biofilm individually and in consortium have been evaluated. In addition to enhancing the stability and strength of the matrix, the components of the enteric biofilm influence bacterial virulence and transmission. Most notably, certain components of the matrix are recognized as pathogen-associated molecular patterns. Systemic recognition of enteric biofilms leads to the activation of several proinflammatory innate immune receptors, including the Toll-like receptor 2 (TLR2)/TLR1/CD14 heterocomplex, TLR9, and NLRP3. In the model ofSalmonella entericaserovar Typhimurium, the immune response to curli is site specific. Although a proinflammatory response is generated upon systemic presentation of curli, oral administration of curli ameliorates the damaged intestinal epithelial barrier and reduces the severity of colitis. Furthermore, curli (and extracellular DNA) of enteric biofilms potentiate the autoimmune disease systemic lupus erythematosus (SLE) and promote the fibrillization of the pathogenic amyloid α-synuclein, which is implicated in Parkinson's disease. Homologues of curli-encoding genes are found in four additional bacterial phyla, suggesting that the biomedical implications involved with enteric biofilms are applicable to numerous bacterial species.

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“…In addition to fostering biofilm development, curli activation has been demonstrated to dampen the inflammatory response (35) and thus may aid in establishing chronic GB infection by helping to provide an environment conducive to bacterial growth and biofilm formation. Although curli expression in Salmonella and E. coli has been detected in the host (10,11,(36)(37)(38), the subject is still hotly debated (8), as it had been thought to be expressed only at 28°C to 30°C (29,39,40). Our work could potentially point to a new avenue of study regarding the role of curli fimbriae in vivo, as past studies in Salmonella have not examined bile and biofilms as factors contributing to curli activation.…”

Section: Discussionmentioning

confidence: 91%

Human Bile-Mediated Regulation of Salmonella Curli Fimbriae

et al. 2019

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Typhoid fever is caused primarily by Salmonella enterica serovar Typhi. Approximately 3% to 5% of individuals infected with S. Typhi become chronic carriers with the gallbladder (GB) as the site of persistence, as gallstones within the GB are a platform on which the bacteria form a biofilm. S. Typhi is a human-restricted pathogen; therefore, asymptomatic carriers represent a critical reservoir for further spread of disease. To examine the dynamics of the Salmonella biofilm during chronic carriage, the human gallstone (GS) environment was simulated by growing biofilms on cholesterol-coated surfaces in the presence of bile, and the transcriptional profile was determined. Some of the most highly activated genes corresponded to the curli fimbria operon, with the major structural component csgA upregulated >80-fold. The curli protein polymer is a major component of the extracellular matrix (ECM) in Salmonella biofilms. The upregulation of curli fimbriae by human bile was validated through reverse transcription-quantitative PCR (qRT-PCR), microscopy, and Western blotting. Interestingly, this activation appears human specific, as qRT-PCR showed repression of csgA in biofilms grown in mouse or ox bile. Comparative transcriptional studies of the two divergent csg operons suggest an early activation of both operons in minimal medium complemented with glucose that quickly diminishes as the biofilm matures. However, in the presence of human bile, there is a modest activation of both operons that steadily increases as the biofilm matures. Understanding the effect of the GB environment on key biofilm-associated factors can help target antibiofilm therapeutics or other preventative strategies to eradicate chronic carriage. IMPORTANCE Typhoid fever is caused by Salmonella enterica serovar Typhi, and 3% to 5% of patients become chronic gallbladder (GB) carriers (also known as “Typhoid Marys”). We have previously demonstrated a role for Salmonella biofilm formation on gallstones as a primary mechanism of carriage. In this study, we found that the important biofilm extracellular matrix component curli fimbria is induced in an in vitro human GB model system. This induction is specific to human bile and increases as the biofilm matures. We also found that the biofilm and curli regulator CsgD play a key role in this observed induction. This work further enhances our understanding biofilm-mediated chronic carriage and provides a potential target for eliminating persistent GB infection by S. Typhi.

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Curli fimbria: an Escherichia coli adhesin associated with human cystitis

Cited by 34 publications

References 21 publications

Effect and Analysis of Bacterial Lysates for the Treatment of Recurrent Urinary Tract Infections in Adults

Effect and Analysis of Bacterial Lysates for the Treatment of Recurrent Urinary Tract Infections in Adults

Curli-Containing Enteric Biofilms Inside and Out: Matrix Composition, Immune Recognition, and Disease Implications

Human Bile-Mediated Regulation of Salmonella Curli Fimbriae

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