Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities

Zhou, Lisha; Li, Hui; Zhang, Ying; Han, Shun; Xu, Hui

doi:10.1016/j.bjm.2016.01.001

Cited by 66 publications

(32 citation statements)

References 30 publications

(48 reference statements)

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“…Studies on Sphingomonas that we have seen are mainly distributed in the eld of environmental science. Sphingomonas are often isolated from petroleum contaminated soils due to their unique abilities to degrade polycyclic aromatic hydrocarbons (PAHs) [28] , which has been recognized as a de nite carcinogen in the world. While another study [29] suggests that both HPV infection and PAHs are critical factors in the development of cervical cancer.…”

Section: Discussionmentioning

confidence: 99%

Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

Zhang

et al. 2020

Preprint

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Background: High-risk human papilloma virus (hrHPV) is regarded as the main causal factor of cervical precancer and cancer when persistent infection is left untreated. Previous studies have declared that HPV is associated with microecological environment of lower genital tract but haven’t discriminate vaginal microbiota from that of cervix. Objective: To analyze the distinction between vaginal and cervical microbiota in high-risk HPV(+) Chinese women. Methods: One hundred participants were recruited including 20 healthy women with HPV (-), 32 with other hrHPV (+), 38 subjects with HPV16/18 (+) and 10 with cervical carcinoma, demographics of whom were collected and analyzed. Vaginal and cervical microbiota were separately tested through next-generation sequencing technology (NGS) targeting the variable region (V3-V4) of bacterial ribosome 16S rRNA gene. Results: 1. Analysis of demographics demonstrated that hrHPV infected women tend to be accustomed to vaginal douching (p =0.001), show more frequent usage of sanitary pads (p =0.007), have more sex partners (p =0.047), be more sexually active (p =0.025), have more diverse ways of contraception (p =0.001) and history of vaginitis (p =0.002). 2. NGS identified microbial diversity of cervical microbiota was much higher than that of vagina with significantly increased Proteobacteria and decreased Lactobacillus. Variation of cervical microbiota of hrHPV(+) subjects partly similar to vaginal microbiota but had its unique features. Sphingomonas of α-Proteobacteria, almost invisible in the vagina, was more frequent at normal cervix whereas decreased remarkably at hrHPV(+) cervix. Reversely, γ-Proteobacteria showed a significant positive correlation with HPV16/18 and cervical cancer. BV related anaerobes like Gardnerella, Prevotella, Atopobium and Sneathia showed similar changes in both vaginal and cervical microbiota of hrHPV(+) women and did not exhibit cervical specificity. Conclusions: Cervical microbiota has its uniqueness from that of vagina in bacterial communities presenting a higher proportion of Proteobacteria, of which Sphingomonas is potentially predictive of a health guardian of hrHPV while Pseudomonas the opposite.

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Section: Discussionmentioning

confidence: 99%

Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

Zhang

et al. 2020

Preprint

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“…Members of this genus are able to degrade polycyclic aromatic hydrocarbons and are frequently isolated from petroleum-contaminated soils; they are important for in situ bioremediation (Balkwill et al, 1997;Zhou et al, 2016). The genus Chitinophaga was also found under the Atlantic Forest vegetation.…”

Section: Discussionmentioning

confidence: 99%

Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant

Castro

Souza

Rufini

et al. 2017

Rev. Bras. Ciênc. Solo

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How to cite: Castro JL, Souza MG, Rufini M, Guimarães AA, Rodrigues TL, Moreira FMS. Diversity and efficiency of rhizobia communities from iron mining areas using cowpea as a trap plant. Rev Bras Cienc Solo. 2017;41:e0160525. https://doi.org/10.1590/18069657rbcs20160525Copyright: This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided that the original author and source are credited. Division -Soil Processes and Properties ABSTRACT:Mining is an important economic activity. However, its impact on environment must be accessed, mainly on relevant processes for their sustainability. The objective of this study was to evaluate the diversity and efficiency of symbiotic nitrogen fixing bacterial communities in soils under different types of vegetation in the Quadrilátero Ferrífero: ironstone outcrops, Atlantic Forest, neotropical savanna, and a rehabilitated area revegetated with grass. Suspensions of soil samples collected under each type of vegetation were made in a saline solution to capture rhizobia communities that were then inoculated on cowpea [Vigna unguiculata (L.) Walp.], which was used as a trap plant. The symbiotic efficiency of the communities was evaluated in a greenhouse experiment and the data obtained were correlated to the chemical and physical properties of the soils under each type of vegetation. At the end of the experiment, the bacteria present in the nodules were isolated to evaluate their diversity. The highest numbers of nodules occurred in the treatment inoculated with soil samples from rehabilitated area revegetated with grass and neotropical savanna vegetation, and the lowest numbers were observed in the treatment inoculated with soil samples from ironstone outcrops and Atlantic Forest. In relation to root dry matter, the treatment inoculated with soil samples from Neotropical savanah was superior to those inoculated with soil samples from the other areas; already, in relation to the shoot dry matter, no significant difference among the treatments was observed. The soil properties with the greatest influence on the microbial communities were Al 3+ content, considered as high in the Atlantic Forest and neotropical savanna vegetation, as intermediate in the iron outcrops, and as very low in the rehabilitated area revegetated with grass; organic matter, considered as very high in the ironstone outcrops and neotropical savanna, as high in the Atlantic Forest, and as low in the rehabilitated area revegetated with grass; and the pH, with intermediate acidity level in the rehabilitated area revegetated with grass, high level of acidity in the iron outcrops and neotropical savanna, and very high acidity in the Atlantic Forest. After isolation of the nodules, 380 bacterial strains were obtained and separated into 27 groups by cultural characterization analysis. Genetic diversity was evaluated by the 16S rRNA gene partial sequencing of 156 strains, which identifie...

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“…Independent triplicates were performed for the six conditions, for a total of 18 pots. Each pot had 40 ryegrass seedlings [29].…”

Section: Soil Remediation Testmentioning

confidence: 99%

Impact of biochar on the metabolic networks of a PHE-degrading microbial community and its influencing mechanism on soil biogeochemical cycles

Li¹,

Li²,

Song³

et al. 2020

Preprint

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Background: Straw pyrolysis into biochar are beneficial for resource recovery and soil improvement. However, little is known about how biochar influences polycyclic aromatic hydrocarbons (PAHs) metabolic pathways and biogeochemical cycles in PAH-contaminated agricultural soil. Here we assessed the influence of biochar and bacterial inoculant on the soil physicochemical properties, the microorganisms involved in the metabolism of PAH and C, N, P and S cycling.Results: The addition of biochar and bacterial inoculant improved soil fertility and crop nutrition. The community metabolism of phenanthrene was revealed by modeling a gene network based on shotgun metagenomes. Biochar addition in soil promoted the abundance of various phenanthrene-degrading microorganisms involved in multiple steps of phenanthrene catabolism, thereby promoting phenanthrene degradation. Meanwhile, biochar addition increased nitrate reduction and degradation of relatively easily decomposable organic carbon, including cellulose, but it also inhibited lignin and chitin degradation and C and N fixation, while the addition of bacterial inoculant partially mitigated biochar’s inhibitory effects in element cycle and inhibited N2O emission, which alleviated the greenhouse effect.Conclusions: When bioremediating PAH-contaminated soil, recommendation is to use biochar combined with functional microorganism. This work contributes to expand the current knowledge of the treatment of contaminated soil and provide some empirical evidence for the treatment of contaminated soil by biochar and bacterial inoculant.

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Sphingomonas from petroleum-contaminated soils in Shenfu, China and their PAHs degradation abilities

Cited by 66 publications

References 30 publications

Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

Distinction Between Vaginal and Cervical Microbiota in High-risk Human Papilloma Virus Infected Women in China

Diversity and Efficiency of Rhizobia Communities from Iron Mining Areas Using Cowpea as a Trap Plant

Impact of biochar on the metabolic networks of a PHE-degrading microbial community and its influencing mechanism on soil biogeochemical cycles

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