The improvement of competitive saprophytic capabilities of Trichoderma species through the use of chemical mutagens

Abstract

The antagonistic potential of Trichoderma strains was assayed by studying the effect of their culture filtrate on the radial growth of Sclerotium rolfsii, the causal agent of chickpea collar rot. Trichoderma harzianum-1432 (42.2%) and Trichoderma atroviride (40.3%) were found to be strong antagonists. To enhance their antagonistic potential, mutagenesis of these two selected strains was performed. Two mutants, Th-m1 and T. atroviride m1, were found to be more effective than their parent strains. The enzymatic activities of the selected parent and mutant strains were assayed, and although both mutants were found to have enhanced enzymatic activities compared to their respective parent strains, Th-m1 possessed the maximum cellulase (5.69 U/mL) and β-1,3-glucanase activity (61.9 U/mL). Th-m1 also showed high competitive saprophytic ability (CSA) among all of the selected parent and mutant strains, and during field experiments, Th-m1 was found to successfully possess enhanced disease control (82.9%).

Host-Induced Gene Silencing of Rice Blast Fungus Magnaporthe oryzae Pathogenicity Genes Mediated by the Brome Mosaic Virus

“…Chemical mutagenesis and Agrobacterium T-DNA or transposons insertions are the most known strategies for studying loss-of-function in fungi, which have been widely used and are also a pivotal choice for the model fungi M. oryzae [ 37 , 38 , 39 ]. By contrast, RNAi is a reverse genetics tool for studying functional genomics that presents many advantages and avoids many disadvantages of traditional approaches to functional analyses in M. oryzae [ 24 , 30 , 35 ]: (1) it is rapid; (2) it can avoid time-consuming stable fungi transformation; and (3) it can work for the studies of M. oryzae .…”

Chitosan and nematophagous fungi for sustainable management of nematode pests

“…are generalist fungi found in soil and roots. They can act as saprophytes (Rashmi et al, 2016), endophytes (Tseng et al, 2020), and pathogens of fungi and nematodes (Sahebani and Hadavi, 2008). This versatility allows them to survive well in the rhizosphere.…”

Pathogenicity and biological control of Bayoud disease by Trichoderma longibrachiatum and Artemisia herba-alba essential oil

“…where C is radial growth measurement of the pathogen in the control and T is the radial growth of the pathogen in the presence of T. longibrachiatum (Benabbes et al, 2015b;Rashmi et al, 2016).…”

“…Furthermore, Trichoderma species are freeliving fungi that are highly interactive in root, soils, and foliar environments, from their effective antagonistic capacity, these species are used as potential candidates for biological control of plant diseases (Kredics et al, 2003;Reino et al, 2008). Some strains of Trichoderma colonize the root surfaces and penetrate the epidermis, which improves root growth, productivity, and resistance to biotic and abiotic stress and assimilation of nutrients (Harman et al, 2004).…”