Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Ferreira, Michele Berger; de‐Paris, Fernanda; Paiva, Rodrigo Minuto; Nunes, Luciana Neves

doi:10.1016/j.bjid.2018.09.005

Cited by 12 publications

(13 citation statements)

References 40 publications

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“…In contrast to culture which is a considered as a gold standard tool, 77.3% more detection capacity was offered by PCR [21]. In agreement to our study, other studies in Brazil (3.8% vs. 29.2%) [22], Iran (27.7% vs. 43.8%) [23], China (0% vs. 50.7%) [24] and Jimma, Ethiopia (3% vs. 33%) were also indicated the sensitivity of PCR over culture [25]. � Normal values: WCC = 0-5/μL, CSF Protein = 40-100 mg/dl and CSF glucose = 40-75 mg/dl [13,14].…”

Section: Discussionsupporting

confidence: 91%

Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia

et al. 2020

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Meningitis is one of the top ten causes of death among Ethiopian infants. Group B streptococcus (GBS) has emerged as a leading cause of meningitis in neonates and young infants, resulting in high mortality. Despite this, there is no report on GBS associated meningitis in Ethiopia where infant meningitis is common. Hence, the aim of this study was to determine the proportion of GBS associated meningitis among Ethiopian infants. PCR was prospectively used to detect GBS in culture-negative cerebrospinal fluid (CSF) samples, which were collected from infants suspected for meningitis, at Tikur Anbessa specialized hospital, Ethiopia, over a one-year period. GBS was detected by PCR in 63.9% of culture-negative CSF samples. Out of the 46 GBS positive infants, 10.9% (n = 5) of them died. The late onset of GBS (LOGBS) disease was noted to have a poor outcome with 3 LOGBS out of 5 GBS positive samples collected from patients with the final outcome of death. PCR was advantageous in the identification of GBS in culture-negative CSF samples. GBS was detected in 64% of the CSF samples from infants with meningitis compared with zero-detection rate by culture.

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Section: Discussionsupporting

confidence: 91%

Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia

et al. 2020

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“…Two studies, from Iran and Brazil, were identified, 12,14 both favoring a PCR approach over microbiological cultures, demonstrating higher detection rates. Ferreira et al 16 also found that of 130 specimens, 23 (18%) were reported positive by conventional PCR, 38 (29%) by real‐time PCR, and 5 (3.8%) by culture. The real‐time PCR assays had a sensitivity of 100% and specificity of 74% when compared with the cultures.…”

Section: Resultsmentioning

confidence: 93%

A scoping review of maternal antibiotic prophylaxis in low‐ and middle‐income countries: Comparison to WHO recommendations for prevention and treatment of maternal peripartum infection

Jury

Thompson

Hirst

2021

Intl J Gynecology & Obste

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Background: Sepsis is a leading cause of maternal death. Antimicrobials save lives, but inappropriate overuse increases risk of antimicrobial resistance. Objective: A scoping review comparing peripartum prophylactic antimicrobial use in low-and middle-income countries (LMICs) with WHO recommendations for prevention and treatment of maternal peripartum infection. Search strategy: Medline, Embase, Global Health, LILACS and the WHO Library databases were searched. Selection criteria: Publications from LMICs since 2015 describing maternal prophlyactic antibiotics for group B streptococcus (GBS), preterm-prelabor rupture of membranes (PPROM), cesarean section, manual placental removal, and third/fourthdegree perineal tears.Data collection and analysis: Publications were screened, and duplicates were removed. A scoping review was conducted using PRISMA guidelines. Owing to study heterogeneity, a narrative synthesis was performed.Main results: Of 1886 studies, 27 studies from 13 countries involving 43 774 women met the eligibility criteria. Polymerase chain reaction screening for GBS is feasible, though limited financially. In PPROM, up to 42% of GBS isolates demonstrated erythromycin resistance. Evidence around cesarean section antimicrobial prophylaxis largely supports WHO recommendations; however, prolonged or multidrug regimens were reported. Conclusion:There is limited evidence to challenge current WHO recommendations to prevent peripartum infection in LMICs. However, implementation challenges exist.Given the emergence of antimicrobial resistance, research is needed to ensure that peripartum prophylactic antimicrobial choices remain effective.

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“…Currently, bacterial culture is the gold standard for the identification of GBS infection, but it is timeconsuming with limited sensitivity and vulnerable to interference [18]. Also, real-time qPCR requires expensive equipment and only quantifies nucleic acid relatively.…”

Section: Discussionmentioning

confidence: 99%

Development of a droplet digital PCR method for detection of Streptococcus agalactiae

Zeng

Chen

et al. 2020

BMC Microbiol

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Background: Streptococcus agalactiae (GBS) is the causative pathogen of puerperal sepsis in pregnant women and pneumonia, sepsis and meningitis in infants. Infection of GBS is responsible for the increased morbidity in pregnant women and the elderly, and bring challenges to clinical diagnosis and treatment. However, culture-based approaches to detect S.agalactiae is time-consuming with limited sensitivity. Besides, real-time quantitative PCR demands expensive instruments with tedious steps. Thus, we aim to establish a new detection method for more accurate and rapid detection of S.agalactiae. Results: The ddPCR primer targeted the CpsE gene showed better amplified efficiency in the reaction. The limit of detection for GBS DNA with ddPCR was able to reach 5 pg/μL. Moreover, no positive amplified signals could be detected in the reactions which served 11 non-GBS strains DNA as templates. Furthermore, the coefficient of variation of this method was 4.5%, indicating excellent repeatability of ddPCR assay. Conclusions: In our study, ddPCR was performed as a rapid detection of S.agalactiae with high sensitivity and specificity. This technique can promote the accuracy of the diagnosis of GBS infection and provide a scientific basis for clinical treatment.

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Assessment of conventional PCR and real-time PCR compared to the gold standard method for screening Streptococcus agalactiae in pregnant women

Cited by 12 publications

References 40 publications

Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia

Enhanced identification of Group B streptococcus in infants with suspected meningitis in Ethiopia

A scoping review of maternal antibiotic prophylaxis in low‐ and middle‐income countries: Comparison to WHO recommendations for prevention and treatment of maternal peripartum infection

Development of a droplet digital PCR method for detection of Streptococcus agalactiae

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