Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study

A substantial proportion of keratoconjunctivitis is not associated with a detectable adenovirus. The clinical course of those with adenovirus D keratoconjunctivitis is significantly more severe than those with non-adenovirus D species infections or adenovirus-negative keratoconjunctivitis; high viral load at presentation and non-United States origin of participants is associated with poorer clinical outcome.

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“…This study’s adenoviral positive rate by PCR in suspected viral conjunctivitis is similar to our 78% adenoviral PCR positive rate. 23…”

Section: Discussionmentioning

confidence: 99%

Determinants of Outcomes of Adenoviral Keratoconjunctivitis

Lee

Akileswaran

et al. 2018

Ophthalmology

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“…Totally, 88 symptomatic patients were considered for this study. The samples were collected from the lower palpebral conjunctiva using sterile Dacron swab, which was subsequently introduced in a transport media (DMEM) supplemented with antibiotics and antifungal (15). Specimens were stored at −70°C for further processing.…”

Section: Methodsmentioning

confidence: 99%

Frequency of adenovirus serotype 8 in patients with Keratoconjunctivitis, in Ahvaz, Iran

Shafiei

Makvandi

Teimoori

et al. 2019

IJM

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Background and Objectives: Adenoviral keratoconjunctivitis is an extremely frequent ophthalmological disease caused by various serological subtypes of human adenovirus (HAdV) worldwide. Adenoviruses serotypes 8, 11, 19, 37 frequently cause epidemic keratoconjunctivitis (EKC). This study was conducted to evaluate the frequency of adenovirus serotypes in patients with EKC in Ahvaz , Iran. Materials and Methods: Eighty- eight ocular swabs were collected from patients with EKC. The specimens were analyzed for detection of adenovirus by standard PCR. The PCR products were further sequenced and analyzed to determine the serotypes. Results: The study population consisted of 49/88 (55.7%) males and 39/88 (44.3%) females. Among them 25 (51.02%) males and 22 (56.41%) females were positive for HAdV serotype 8 (p= 0.488). Overall forty-seven (53.4%) samples were positive for AdV serotype 8 while forty- one patients (46.59%) were negative for the adenovirus serotypes. Conclusion: The results of this study revealed predominanance of HAdV 8 with high prevalence of 53.4% among patients with Keratoconjunctivitis. Forty- one patients (46.59%) were negative for adenovirus. Still, the role for other related viruses such as enteroviruses need to be investigated in patients with EKC.

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“…Enteroviruses (EVs) and adenoviruses are the main etiologic agents of AHC [1, 2]. In particular, coxsackievirus A24 (CV-A24, member of Enterovirus C species) and enterovirus D70 (member of Enterovirus D species) have been associated with large AHC outbreaks in the past.…”

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confidence: 99%

Coxsackievirus A24 Variant Associated with Acute Haemorrhagic Conjunctivitis Cases, French Guiana, 2017

et al. 2017

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In 2017, numerous cases of acute haemorrhagic conjunctivitis (AHC) were reported in the Caribbean and in South America. Preliminary reports identified adenoviruses and enteroviruses in some patient samples but, until now, none of the etiologic agents have been fully characterized. We report the full-length genomic sequences of 4 coxsackievirus A24 (CV-A24) isolates collected from AHC patients in French Guiana during this outbreak (May and June 2017). These isolates are very closely related and belong to the genotype IV of CV-A24 variant, which consists of strains sampled worldwide during AHC outbreaks in the 2000s and 2010s. No recombination events were detected within the genomic sequences, indicating that members of this genotype have continuously circulated worldwide for more than 10 years without undergoing recombination with other enteroviruses. This unusual trait could be due to their ocular tropism that could impede genetic exchanges between these viruses and other enteroviruses, which replicate mainly in the gut.

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Molecular identification of viral agents associated with acute conjunctivitis: a prospective controlled study

Cited by 12 publications

References 14 publications

Determinants of Outcomes of Adenoviral Keratoconjunctivitis

Determinants of Outcomes of Adenoviral Keratoconjunctivitis

Frequency of adenovirus serotype 8 in patients with Keratoconjunctivitis, in Ahvaz, Iran

Coxsackievirus A24 Variant Associated with Acute Haemorrhagic Conjunctivitis Cases, French Guiana, 2017

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