Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms

Sun, E; Liang, Ge-hong; Wang, Lining; Wei, Wenjie; Lei, Mingde; Song, Shiduo; Han, Renzhi; Wang, Yubao; Wei, Qi

doi:10.1016/j.bjid.2016.04.002

Cited by 30 publications

(27 citation statements)

References 38 publications

(51 reference statements)

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“…Recently, a systematic review reported that fluoroquinolones show comparable effects on the mortality of S. maltophilia infection compared with trimethoprim-sulfamethoxazole, supporting the use of fluoroquinolones in clinical S. maltophilia infections [39], although high rates of resistance are also increasingly being reported. In the current study, the newer fluoroquinolone levofloxacin showed better activity against S. maltophilia than ciprofloxacin (susceptibility rates: 77.9% versus 18.8%, respectively), confirming previous studies [9,37,38].…”

Section: Discussionsupporting

confidence: 89%

“…SXT is the antibiotic of choice for treating S. maltophilia infections, although in recent years, increasing rates of resistance, ranging from 16% to 45%, were reported, especially in CF patients [9,35,36]. Considering available clinical breakpoints, our results confirmed SXT is the most active compound against all the strains tested.…”

Section: Discussionsupporting

confidence: 71%

“…Significance level at Fisher's exact test: * p < 0.05; *** p < 0.001. Resistance profiles according to Magiorakos et al[24]: MDR, multidrug-resistant strains; XDR, extensively drug-resistant strains; PDR, pandrug-resistant strains.Antibiotics 2020,9,15 6 of 15…”

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confidence: 99%

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Clonal Diversity, Biofilm Formation, and Antimicrobial Resistance among Stenotrophomonas maltophilia Strains from Cystic Fibrosis and Non-Cystic Fibrosis Patients

et al. 2020

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The intrinsic antibiotic resistance of Stenotrophomonas maltophilia, along with its ability to form biofilm both on abiotic surfaces and host tissues, dramatically affects the efficacy of the antibiotic therapy. In this work, 85 S. maltophilia strains isolated in several hospital of central Italy and from several clinical settings were evaluated for their genetic relatedness (by pulsed-field gel electrophoresis, PFGE), biofilm formation (by microtiter plate assay), and planktonic antibiotic resistance (by Kirby–Bauer disk diffusion technique). The S. maltophilia population showed a high genetic heterogeneity: 64 different PFGE types were identified, equally distributed in cystic fibrosis (CF) and non-CF strains, and some consisted of multiple strains. Most of the strains (88.2%) were able to form biofilm, although non-CF strains were significantly more efficient than CF strains. CF strains produced lower biofilm amounts than non-CF strains, both those from respiratory tracts and blood. Non-CF PFGE types 3 and 27 consisted of strong-producers only. Cotrimoxazole and levofloxacin were the most effective antibiotics, being active respectively against 81.2% and 72.9% of strains. CF strains were significantly more resistant to piperacillin/tazobactam compared to non-CF strains (90% versus 53.3%), regardless of sample type. Among respiratory strains, cotrimoxazole was more active against non-CF than CF strains (susceptibility rates: 86.7% versus 75%). The multidrug resistant phenotype was significantly more prevalent in CF than non-CF strains (90% versus 66.7%). Overall, the multidrug-resistance level was negatively associated with efficiency in biofilm formation. Our results showed, for the first time, that in S. maltophilia both classical planktonic drug resistance and the ability of biofilm formation might favor its dissemination in the hospital setting. Biofilm formation might in fact act as a survival mechanism for susceptible bacteria, suggesting that clinical isolates should be routinely assayed for biofilm formation in diagnostic laboratories.

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Section: Discussionsupporting

confidence: 89%

Section: Discussionsupporting

confidence: 71%

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Clonal Diversity, Biofilm Formation, and Antimicrobial Resistance among Stenotrophomonas maltophilia Strains from Cystic Fibrosis and Non-Cystic Fibrosis Patients

et al. 2020

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“…Trimethoprim-sulfamethoxazole (STX) is the first-line antimicrobial combination for the treatment of S. maltophilia infections. However, the allergenic reaction, the intolerance, and the increasing rates of resistance limit the use of Trimethoprim-sulfamethoxazole [18,19]. S. maltophilia infections are also difficult to treat due to the ability of this bacterium to form highly structured and multilayered biofilms [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

Antibacterial and Antivirulence Activity of Glucocorticoid PYED-1 against Stenotrophomonas maltophilia

et al. 2020

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Stenotrophomonas maltophilia, an environmental Gram-negative bacterium, is an emerging nosocomial opportunistic pathogen that causes life-threatening infections in immunocompromised patients and chronic pulmonary infections in cystic fibrosis patients. Due to increasing resistance to multiple classes of antibiotics, S. maltophilia infections are difficult to treat successfully. This makes the search for new antimicrobial strategies mandatory. In this study, the antibacterial activity of the heterocyclic corticosteroid deflazacort and several of its synthetic precursors was tested against S. maltophilia. All compounds were not active against standard strain S. maltophilia K279a. The compound PYED-1 (pregnadiene-11-hydroxy-16α,17α-epoxy-3,20-dione-1) showed a weak effect against some S. maltophilia clinical isolates, but exhibited a synergistic effect with aminoglycosides. PYED-1 at sub-inhibitory concentrations decreased S. maltophilia biofilm formation. Quantitative real-time polymerase chain reaction (RT-qPCR) analysis demonstrated that the expression of biofilm- and virulence- associated genes (StmPr1, StmPr3, sphB, smeZ, bfmA, fsnR) was significantly suppressed after PYED-1 treatment. Interestingly, PYED-1 also repressed the expression of the genes aph (3′)-IIc, aac (6′)-Iz, and smeZ, involved in the resistance to aminoglycosides.

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“…Non-fermenting Gram-negative bacilli (NF-GNB) colonization and infection are frequent among critically ill patients. Antibiotic resistance is common in NF-GNB, resulting in inappropriate initial antibiotic treatment and poor outcomes 1,11,12,13 . Several outbreaks of infection have been described including a few reports of infections originating outside the hospital setting 14,15,16,17,18 .…”

Section: Discussionmentioning

confidence: 99%

Stenotrophomonas maltophilia isolates in a tertiary care hospital in Northeastern Brazil

Lemos

Paixão

Santos³

et al. 2019

BJHR

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Introduction: Stenotrophomonas maltophilia (S.maltophilia) has emerged as an important opportunistic, nosocomial pathogen capable of causing respiratory, bloodstream and urinary infections in a debilitated host. Prevention of acquisition and infection depends upon the application of modern infection-control practices. Methods: We conducted a retrospective and observational laboratory-based study on S. maltophilia to evaluate the distribution of these pathogen isolates between February 2008 and December 2012 in a 38-bed intensive care unit in northeastern Brazil. The study began by surveying all positive S. maltophilia cultures processed by the microbiology laboratory during the period of study. The cultures, originating from various types of biological material were processed by Vitek® System. The cultures were processed in protocol in accordance with CLSI-M100-S25. The incidence rate was calculated per 1,000 admissions. Results: A total of 100 sample isolates were tested positive for S. maltophilia. The isolates were obtained from 50.301 hospitalized patients (0.19%). A total of 8.572 positive cultures were detected in different biological materials. Ninety four (94%) isolates were from patients in adult intensive care. Seventy (70%) S. maltophilia isolates were from tracheal cultures. All S. maltophilia isolates were sensitive to Co-trimoxazole and levofloxacin. Conclusions: This study shows the importance of other analytical epidemiologic studies that correlate the presence of S. maltophilia incidence associated with the risk factor, and the outcomes, in our region.

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Antimicrobial susceptibility of hospital acquired Stenotrophomonas maltophilia isolate biofilms

Abstract: Antibiotics could act synergistically to combat the protection conferred to clinical isolates of Stenotrophomonas maltophilia by biofilms. Macrolide antibiotics may be effective where used in combination.

Cited by 30 publications

References 38 publications

Clonal Diversity, Biofilm Formation, and Antimicrobial Resistance among Stenotrophomonas maltophilia Strains from Cystic Fibrosis and Non-Cystic Fibrosis Patients

Clonal Diversity, Biofilm Formation, and Antimicrobial Resistance among Stenotrophomonas maltophilia Strains from Cystic Fibrosis and Non-Cystic Fibrosis Patients

Antibacterial and Antivirulence Activity of Glucocorticoid PYED-1 against Stenotrophomonas maltophilia

Stenotrophomonas maltophilia isolates in a tertiary care hospital in Northeastern Brazil

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