Incidence of virulence determinants in clinical Enterococcus faecalis and Enterococcus faecium isolates collected in Bulgaria

Strateva, Tanya; Atanasova, Д.; Savov, Encho; Petrova, Guergana; Mitov, Ivan

doi:10.1016/j.bjid.2015.11.011

Cited by 85 publications

(78 citation statements)

References 34 publications

(58 reference statements)

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“…There is the similarity of hyaluronidase enzyme in other bacteria such as Streptococcus pyogenes, S. aureus, and Streptococcus pneumonia (11,14). In France, the virulence determinants have evaluated regarding glycopeptide resistance.…”

Section: Discussionmentioning

confidence: 99%

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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Background: The enterococci are responsible for infections including bacteremia and endocarditis which are usually resistant to multiple antibiotics. This nosocomial agent probably harbors putative virulence factors which increases their capability to colonize hospitalized patients. Objectives: This study was aimed in order to find the frequency of various virulence factors in enterococci and their relationship with multidrug resistance (MDR). Methods: The samples were collected from different hospital wards including; Intensive care unit (ICU), cardiac care unit (CCU), pediatrics department, internal wards, and transplantation. The isolates were detected by biochemical tests and in order to determine the antibiotic susceptibility pattern, disk diffusion agar (kirby-bauer) was accomplished. Then, MICs (Minimum inhibitory concentrations) of vancomycin were determined by E-test strips. For molecular examinations and detection of drug resistance genes, the simple polymerase chain reaction was used. The multiplex PCR was used in order to detect virulence factors. Results: Total of 85 isolates were obtained from one university teaching hospital in southeast of Iran. Approximately 95% of isolated which were from urine specimens and 34% of isolates were collected from pediatrics units at hospital. Tetracycline resistance (48%) has been observed with a high frequency and related to the tetM gene. Furthermore, eighteen isolates were recognized as MDR strains that carried vanA, aac (6)-Ie-aph (2)-Ia, ermB, and tetM genes. Among virulence factor genes, asa1 (69%) and gelE (55%) are more frequently observed in both species. In general, we found Enterococcus faecalis strains more prevalent. Also, E. faecium was related to antibiotic resistance genes in nosocomial infection. Conclusions:The data was indicated a high prevalence of multiple antibiotics resistance genes with virulence determinants in enterococci and also considered resistant isolate in pediatrics unit. The current results can be recommended in order to change new strategies for antibiotic therapy, because this serious pathogen is important for treatment and eradication in hospitals. Furthermore, the biofilm formation was regulated and constructed by virulence determinants that could be a candidate for enterococcal treatment.

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Section: Discussionmentioning

confidence: 99%

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Shahraki

Mousavi

2017

Jundishapur J Microbiol

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“…Several virulence factors including enterococcal surface protein (Esp), hyaluronidase (Hyl), gelatinase (GelE), aggregation substance (AS) proteins (Asa1), collagen-binding protein (Ace), and cytolysin (CylA) contribute to the adherence, colonization, evasion of the host immune response, extracellular production of enzymes, pathogenicity, and severity of the infection [56]. Moreover, treatment of enterococcal infections has been clinically challenging due to increasing resistance to a broad range of antimicrobial agents, including β‑lactams, macrolides, fluroquinolones, glycopeptides, and aminoglycosides [47].…”

Section: Introductionmentioning

confidence: 99%

High Incidence of Virulence Factors Among ClinicalEnterococcus faecalisIsolates in Southwestern Iran

et al. 2017

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BackgroundOver the past two decades, enterococci have emerged as an important agent responsible for hospital acquired infection. Several virulence factors contribute to the adherence, colonization, evasion of the host immune response, and pathogenicity and severity of the infection. Enterococcus faecalis is the most common and virulent species causing infections in hospitalized patients. The aim of the present study was to examine the prevalence of genes encoding virulence factors and antimicrobial resistance patterns of E. faecalis strains isolated from hospitalized patients in Shiraz, south west of Iran.Materials and MethodsA total of 51 E. faecalis isolates from the urine, blood, pleural fluid, peritoneal fluid, eye discharge, endotracheal tube (ETT) and transjugular intrahepatic portosystemic shunt (TIPS) specimens of patients were identified by phenotypic and genotypic methods. Antimicrobial sensitivity tests and detection of virulence factors were performed using standard methods.ResultsThe efa and asa1 were the most frequently detected gene (100%) among the isolates, followed by esp (94.1%), ace (90.2%), gelE (80.4%), cylA (64.7%), and hyl (51%). More than half of the isolates (52.9%) were high level gentamicin resistant (HLGR). Vancomycin resistance was observed among 23 (45.1%) isolates. The lowest antimicrobial activity was related to erythromycin (3.9%), tetracycline (5.9%) and ciprofloxacin (9.8%). No isolate was found resistant to fosfomycin and linezolid.ConclusionOur data indicated a high incidence of virulence factors among E. faecalis strains isolated from clinical samples. Colonization of drug resistant virulent isolates in hospital environment may lead to life threatening infection in hospitalized patients. Therefore, infection control procedures should be performed.

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“…Dicho microorganismo está principalmente relacionado con los casos de fracaso endodóntico, ya que presenta características morfológi-cas y factores de virulencia que le permiten sobrevivir en condiciones extremas dentro del conducto radicular. 13,14 El propósito de este estudio fue evaluar la microfiltración bacteriana de E. faecalis en tres técnicas de obturación endodóntica: la compactación lateral, la compactación vertical caliente (System B y Obtura II) y la técnica de cono único, buscando la técnica que proporcione mejor sellado del conducto radicular para nuestros pacientes.…”

Section: Preparation Of Samplesunclassified

“…This microorganism is linked to endodontic failure due to its morphological characteristics and virulence which allow it to survive in extreme conditions within the root canal. 13,14 The purpose of this study was to evaluate bacterial microleakage of E. faecalis in three endodontic obturation techniques: lateral compaction, warm vertical compaction (System B and Obtura II), and single cone, searching for the technique that provides patients with the best possible root canal sealing.…”

Section: Introductionmentioning

confidence: 99%

Coronal Microleakage Of Enterococcus Faecalis In Three Types Of Endodontic Filling (Warm Vertical Compaction, Lateral Compaction, And Single Cone)

Gomez

Reyes

Pérez

et al. 2017

Rev. Fac. Odontol. Univ. Antioq.

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RESUMEN. Introducción: un adecuado sellado de la obturación endodóntica es crucial para el éxito del tratamiento, ya que impide la entrada de microorganismos y/o el crecimiento de los mismos en caso de persistir en la anatomía radicular. El propósito de este estudio fue determinar CORONAL MICROLEAKAGE OF ENTEROCOCCUS FAECALIS IN THREE TYPES OF ENDODONTIC FILLING (WARM VERTICAL COMPACTION, LATERAL COMPACTION, AND SINGLE CONE) MICROFILTRACIÓN CORONAL DE ENTEROCOCCUS FAECALIS EN TRES TIPOS

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Incidence of virulence determinants in clinical Enterococcus faecalis and Enterococcus faecium isolates collected in Bulgaria

Cited by 85 publications

References 34 publications

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

Determination of Virulence Factors in Clinical Multidrug Resistance Enterococci Isolates at Southeast of Iran

High Incidence of Virulence Factors Among ClinicalEnterococcus faecalisIsolates in Southwestern Iran

Coronal Microleakage Of Enterococcus Faecalis In Three Types Of Endodontic Filling (Warm Vertical Compaction, Lateral Compaction, And Single Cone)

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