Co-transmission of Rahnella aquatilis between hospitalized patients

Abstract

Rahnella aquatilis is an environmental Gram-negative bacillus that is rarely reported as human pathogen, being mainly associated with infections in immunocompromised patients. Herein we describe two cases of R. aquatilis isolates recovered from endotracheal aspirate cultures of different patients in a tertiary hospital located in the city of São Paulo, Brazil. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry and 16S rDNA gene sequencing were performed to confirm bacterial identification after the isolates being erroneously identified as Pantoea spp. by automated system. Both isolates showed the same PFGE pattern and presented the β-lactamase encoding gene blaRAHN-1, responsible for resistance to cephalothin. The isolates were susceptible to broad-spectrum cephalosporins, carbapenems, fluoroquinolones, aminoglycosides, and polymyxin B. This report shows the presence and transmission of uncommon bacteria in the nosocomial environment and alerts us about the need for new tools of correct microbiologic diagnosis.

Identification ofatpDas an optimal reference gene to explore antibiotic resistance and stress tolerance inRahnella aquatilis

“…; Martins et al . ). Many isolates of R. aquatilis can promote plant growth and health, produce the polysaccharides levan and lactan, and bio‐remediate heavy metal pollution (Matsuyama et al .…”

“…Recently, a few clinical isolates of R. aquatilis have been found to be associated with human infections and resistant to antimicrobial agents (Martins et al . ). This has caused public concerns on the emergence of R. aquatilis as a clinically significant pathogen, especially in immunocompromised patients (Martins et al .…”

“…This has caused public concerns on the emergence of R. aquatilis as a clinically significant pathogen, especially in immunocompromised patients (Martins et al . ). Consequently, there has been a growing interest in investigations of antimicrobial resistance and environmental adaptation in R. aquatilis .…”

Bacteriuria with Unusual Uropathogens in Diabetics in Thi-Qar Province

“…The Rahnella genus comprise three species: R. aquatilis, R. genomospecies 2, and R. genomospecies 3, which can only be distinguished using molecular techniques (Martins et al, 2015). As it been described by (Al-Bayatti et al, 2012) ,that drinking water of Tigris river source may contaminated by Rahnella aquaitilis and possible transferred to human by using the water source.…”