Detection of GB virus C/hepatitis G markers in Chinese voluntary blood donors

Xiao, Wei Dong; Lin, Fen; Sun, Pengfei; Ma, Li; Li, Changqing

doi:10.1016/j.bjid.2014.02.001

Cited by 4 publications

(7 citation statements)

References 7 publications

(5 reference statements)

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“…The prevalence of GBV-C infection is greater among patients undergoing hemodialysis compared with blood donors or healthy individuals in the same region. In several countries, the reported prevalence of GBV-C markers ranged from 0.2-24.6% in blood donors (33)(34)(35) and ranged from 3.9-26.5% in patients undergoing hemodialysis (1)(2)(3)(4)(5)36). In the present study, the overall prevalence of GBV-C infection was 57.1%, which is similar to the prevalence of 55% reported in Yogyakarta in 1996 (7).…”

Section: Discussionsupporting

confidence: 78%

Prevalence and genotypic distribution of GB virus C and torque teno virus among patients undergoing hemodialysis

Rinonce

Yano

Utsumi

et al. 2017

Molecular Medicine Reports

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Patients undergoing hemodialysis are at increased risk of infection with blood-borne viruses, including GB virus C (GBV-C) and torque teno virus (TTV). However, the prevalence and genotypic distribution of these viruses in the assessed patients undergoing hemodialysis remains unclear. The present study investigated these issues and the possibility of nosocomial transmission among patients undergoing hemodialysis in a unit in Yogyakarta, Indonesia. GBV-C RNA was detected in 92/161 patients (57.1%) by nested reverse-transcription polymerase chain reaction. Phylogenetic analysis of the 5'-untranslated region (UTR) classified the GBV-C isolates into genotypes 6 (85%), 2 (8%), 4 (6%), and 3 (1%). TTV DNA was detected in all patients by the amplification of the 5'-UTR and open reading frame-1 (ORF1) by nested and semi-nested polymerase chain reaction. Phylogenetic analysis based on the ORF1 revealed that genotype 1 was dominant (84%), followed by genotypes 2 (10%) and 3 (6%). The greater prevalence of GBV-C genotype 6 in patients undergoing hemodialysis compared with the general population and the identical sequences observed in multiple isolates provided strong evidence of patient-to-patient transmission. The prevalence of TTV in hemodialysis patients was similar to that observed in the general population, and only one pair of TTV isolates was identical. These results indicated that nosocomial infection was not the main cause of the high prevalence of TTV in patients undergoing hemodialysis. In conclusion, GBV-C and TTV infections are common in patients undergoing hemodialysis in Yogyakarta, Indonesia, and transmission is likely to be nosocomial in the case of GBV-C infection.

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Section: Discussionsupporting

confidence: 78%

Prevalence and genotypic distribution of GB virus C and torque teno virus among patients undergoing hemodialysis

Rinonce

Yano

Utsumi

et al. 2017

Molecular Medicine Reports

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“…Following a more detailed analysis of HPgV seroprevalence reported in Table 1 for each continent and country, we noted that the serologic assay positivity ranges from 4.9% (Mastouri et al, 2005) to 31.9% (Sathar et al, 1999) and the molecular assay positivity ranges from 5% (Omar et al, 2006) to 18.9% (Sathar et al, 1999) in Africa; in the Americas, no data about seroprevalence is available (with the exception of the 7.3% reported in a Canadian study on identity unlinked and short-term deferred donors (Giulivi et al, 2000)) and the molecular assay positivity ranges from 1.5% (Linnen et al, 1996) to 10% (Lampe et al, 1998); in Asia, the seroprevalence ranges from 1% (Ramezani et al, 2008) to 11% (Xiao et al, 2014) and the molecular assay positivity ranges from 0% (Arankalle et al, 2001;Desai et al, 2004;Kalkan et al, 2005;Barusruk et al, 2006;Ramezani et al, 2008;Deshpande et al, 2013) to 46.7% (Kar et al, 2000); in European countries, the seroprevalence ranges from 9.5% (Mercier et al, 1999) to 24.2% (Brojer et al, 1999) and the molecular assay positivity ranges from 1% (Minton et al, 1998) to 10% (Anastassopoulou et al, 2000); the 10.8% (Moaven et al, 1996) of seroprevalence rate was reported in healthy Australian blood donors and the molecular assay positivity ranges from 2.6% (Hyland et al, 1998) to 4% (Moaven et al, 1996) in the same population.…”

Section: Hpgv In Blood Donorsmentioning

confidence: 99%

“…Previous studies had shown a higher HPgV prevalence rate in blood donors with elevated ALT levels in comparison to those with normal ALT (Moaven et al, 1996;Björkman et al, 1998), but these diff erences were not statistically signifi cant. Moreover, more recent fi ndings suggest that there was no signifi cant diff erence in the activity of liver enzymes in HGV/HPgV RNA-positive donors compared to a control group of healthy donors (Hitzler et al, 2004;Xiao et al, 2014). Similar fi ndings were found in patients with documented post-transfusion HPgV-related hepatitis in whom the plasma ALT peak and the peak viral titers may be discordant, suggesting that HPgV may not be responsible for the rise in ALT levels (Uygun et al, 2000;Lyra et al, 2005;Stapleton et al, 2014), especially in individuals without risk factors for acquiring parenterally transmitted agents (Feucht et al, 1997).…”

Section: Hpgv In Blood Donorsmentioning

confidence: 99%

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The human pegivirus: A new name for an “ancient” virus. Can transfusion medicine come up with something new?

Marano¹,

Franchini²,

Farina³

et al. 2017

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Human pegivirus (HPgV, formerly called GB virus C/hepatitis G virus) is a poorly understood RNA virus of the Flaviviridae family. The HPgV infection is common worldwide and the virus is likely transmitted by blood products. At this time, no causal association between HPgV and human diseases has been identified. While waiting for new findings to better understand the Pegivirus genus, the aim of our narrative review is to discuss the currently available information on HPgV focusing on its prevalence in blood donors and its potential threat to transfusion safety.

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“…The prevalence of HPgV varies in different countries and among the various subjects. The HPgV prevalence was reported to be as low as 0.13% among a healthy blood donors in China, whereas 88.8% of the HIV-1 positive patients were infected with HPgV in Indonesia [8, 19]. In China, high prevalence of HPgV infection was seen among IDUs and MSM [4, 9, 20].…”

Section: Introductionmentioning

confidence: 99%

Effect of human pegivirus route of transmission on the genetic distribution of the virus: an institution based cross-sectional study

Taklual

Tang

2019

Virol J

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Introduction Human pegivirus (HPgV), formally called GB virus C (GBV-C), is a member of the pegivirus genus in Flaviviridae family. High prevalence of HPgV infection is seen among sex workers, blood transfusion recipients and intravenous drug users (IDUs). So far, there are seven genotypes and many subtypes identified in different countries. The predominant genotype in Asia including China is genotype 3, although genotype 7 has been reported recently in China. The aim of this study was to evaluate the effect of the transmission routes of HPgV infection on the genotype distribution of the virus, to determine the prevalence rate, and identify the dominant genotype among men who have sex with men (MSM) and IDUs co-infected with human immunodeficiency virus type one (HIV-1) in Guangzhou, China. Methods A total of 131 MSM and 70 IDUs co-infected with HIV-1 were randomly selected in Guangdong Dermatology Hospital. HPgV RNA was detected by nested reverse transcriptase polymerase chain reaction (RT-PCR) using primers. The PCR products were sequenced and phylogenetically analyzed by using MEGA6.06 version software to determine the genotypes. Chi-square and Fisher exact test were implemented for comparing the proportion between different variables. Results The prevalence of HPgV infection was 32.9% among IDUs and 18.3% in MSM with a statistically significant difference between the two groups ( p = 0.02). In IDU group, 82.6% infected with genotype 3 and the rest (17.4%) were categorized to genotype 7. Similarly, in MSM group, 83.3% belonged to genotype 3, and the remaining 16.7% were classified as sub-genotype 2a and 2b. Conclusion In Guangzhou, China, the prevalence rate of HPgV infection in IDUs was higher than MSM. The dominant genotype in the two groups was genotype 3. Our results indicated that routes of transmission did not affect the genotype distribution but did affect the prevalence rate of HPgV infection.

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Detection of GB virus C/hepatitis G markers in Chinese voluntary blood donors

Cited by 4 publications

References 7 publications

Prevalence and genotypic distribution of GB virus C and torque teno virus among patients undergoing hemodialysis

Prevalence and genotypic distribution of GB virus C and torque teno virus among patients undergoing hemodialysis

The human pegivirus: A new name for an “ancient” virus. Can transfusion medicine come up with something new?

Effect of human pegivirus route of transmission on the genetic distribution of the virus: an institution based cross-sectional study

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