Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China

Yuan, Kai; Zhong, Zhaoming; Zhang, Qiang; Xu, Shu-chai; Chen, Jianting

doi:10.1016/j.bjid.2013.01.013

Cited by 8 publications

(2 citation statements)

References 27 publications

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“…It allows for a rapid and accurate diagnosis of TB (10). ii) Detection of metabolites: a) In vitro interferon (IFN) test-when T-lymphocytes come into contact with mycobacterium, the lymph cells secrete a significant amount of IFN-γ; b) T-SPOT technology of ELISA (11); iii) PCR technology to amplify the DNA of the TB mycobacterium (including repeated sequencing of IS6110), and the results may be obtained within a few hours (12).…”

Section: Discussionmentioning

confidence: 99%

Misdiagnosed and mismanaged atypical spinal tuberculosis: A case series report

Feng

Yang

et al. 2019

Exp Ther Med

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Compared to other diseases, early or atypical spinal tuberculosis (TB) is prone to being misdiagnosed due to the lack of specific clinical manifestations and misleading negative results of various laboratory analyses and imaging examinations. Various methods for diagnosing spinal TB have become research hotspots. Recently, it has been proposed that effective vaccination is a mainstay of long-term policies to combat and control the TB epidemic. However, some new TB vaccines require further evaluation and clinical trials to demonstrate their efficiency and safety. Thus, early diagnosis and effective treatment are the other essential long-term strategies for controlling the TB epidemic immunization is closely associated with the diagnosis of spinal TB, which will become the focus of future research and set the direction for future developments. The present study provides a case series and a literature review of the characteristics of spinal TB to provide guidance for the diagnosis, treatment and prognosis of spinal TB.

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Section: Discussionmentioning

confidence: 99%

Misdiagnosed and mismanaged atypical spinal tuberculosis: A case series report

Feng

Yang

et al. 2019

Exp Ther Med

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“…In recent years, an enzyme-linked immunospot (ELISPOT) assay has been commercialised (T-SPOT.TB; Oxford Immunotec, Oxford, UK) that is based on the detection of interferon-γ released by activated T lymphocytes. The ELISPOT assay has been demonstrated to provide useful support in the diagnosis of osteoarticular TB with high sensitivity and specificity [155][156][157][158].…”

Section: Level Of Evidence:mentioning

confidence: 99%

Joint EANM/ESNR and ESCMID-endorsed consensus document for the diagnosis of spine infection (spondylodiscitis) in adults

Lazzeri

Bozzao

Cataldo

et al. 2019

Eur J Nucl Med Mol Imaging

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Purpose Diagnosis of spondylodiscitis (SD) may be challenging due to the nonspecific clinical and laboratory findings and the need to perform various diagnostic tests including serologic, imaging, and microbiological examinations. Homogeneous management of SD diagnosis through international, multidisciplinary guidance would improve the sensitivity of diagnosis and lead to better patient outcome. Methods An expert specialist team, comprising nuclear medicine physicians appointed by the European Association of Nuclear Medicine (EANM), neuroradiologists appointed by the European Society of Neuroradiology (ESNR), and infectious diseases specialists appointed by the European Society of Clinical Microbiology and Infectious Diseases (ESCMID), reviewed the literature from January 2006 to December 2015 and proposed 20 consensus statements in answer to clinical questions regarding SD diagnosis. The statements were graded by level of evidence level according to the 2011 Oxford Centre for Evidence-based Medicine criteria and included in this consensus document for the diagnosis of SD in adults. The consensus statements are the result of literature review according to PICO (P:population/patients, I:intervention/indicator, C:comparator/control, O:outcome) criteria. Evidence-based recommendations on the management of adult patients with SD, with particular attention to radiologic and nuclear medicine diagnosis, were proposed after a systematic review of the literature in the areas of nuclear medicine, radiology, infectious diseases, and microbiology. Results A diagnostic flow chart was developed based on the 20 consensus statements, scored by level of evidence according to the Oxford Centre for Evidence-based Medicine criteria. Conclusions This consensus document was developed with a final diagnostic flow chart for SD diagnosis as an aid for professionals in many fields, especially nuclear medicine physicians, radiologists, and orthopaedic and infectious diseases specialists.

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Early diagnosis of spinal tuberculosis

Chen

Lee

et al. 2016

Journal of the Formosan Medical Association

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Spinal tuberculosis (STB) is a common manifestation of extrapulmonary tuberculosis (TB). STB accounts for around 2% of all cases of TB and around 15% of extrapulmonary TB cases. The World Health Organization has proposed a global strategy and targets for TB prevention, care, and control after 2015. Under this strategy, patients will receive standard care according to the recommendations and guidelines after confirmation of STB diagnosis. However, current recommendations and guidelines focus on disease and medication therapy management, and recommendations for early detection or decision-making algorithms regarding STB are lacking. In this review, we identified five key components for early diagnosis: (1) risk factors for STB; (2) common symptoms/signs of STB; (3) significant neuroradiological findings of STB; (4) significant laboratory findings of STB, including positive interferon-γ release assays and nonpyogenic evidence in initial laboratory data; and (5) significant clinical findings of STB. Individualized consideration for each patient with STB is essential, and we hope that the algorithm established in this review will provide a valuable tool for physicians who encounter cases of STB.

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Evaluation of an enzyme-linked immunospot assay for the immunodiagnosis of atypical spinal tuberculosis (atypical clinical presentation/atypical radiographic presentation) in China

Abstract: The ELISPOT assay is a useful adjunct to current tests for diagnosis of atypical spinal TB.

Cited by 8 publications

References 27 publications

Misdiagnosed and mismanaged atypical spinal tuberculosis: A case series report

Misdiagnosed and mismanaged atypical spinal tuberculosis: A case series report

Joint EANM/ESNR and ESCMID-endorsed consensus document for the diagnosis of spine infection (spondylodiscitis) in adults

Early diagnosis of spinal tuberculosis

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